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Stone producer group grow


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#381 WalkingCatfish

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Posted 08 May 2019 - 02:54 PM

Well, a few months ago, I started some tampanensis from a spore print (kindly sent to me by a mycotopian who really knows how to grow these things!).  The mycelium grew vigorously, and I soon found myself with a bunch of colonized rye, and no manure. It was the middle of winter, and I just couldn't score any poo. So, I shrugged and spawned them to coir/verm. They took over the substrate pretty efficiently, but (no surprise!) have only produced a few gnarled fruits with shrunken heads, all spaghetti-thin and crooked as Satan's cock. Here's one of the funnier ones...it's about five inches long.

 

Scrawny tampanensis.jpg

 

So, the fruiting is a joke...but I can tell that the substrate is bulging with stones.  They're bulging up from the substrate all over the place.  I've never grown these before, so what I'd like to know is: when should I throw in the towel, break up the tubs and harvest the sclerotia? 

 

I figure I'll start a new grow during the summer, when the poo-fairy is working her magic in the local cow pastures. :biggrin:



#382 jkdeth

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Posted 08 May 2019 - 03:19 PM

Just some info, although it can be used, tampenensis isn't a dung lover, doesn't require poo to fruit.

Just thought I mention that to make sure you explore other reasons for poor fruiting. Lack of poo was not the cause.
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#383 WalkingCatfish

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Posted 08 May 2019 - 05:05 PM

Oh, thanks JK.  For some reason, I thought I'd remembered that Deleena24 was growing them on horse dung, and just assumed that was my problem

 

Hmm...I guess I should do my homework.  If it's not a manure deficiency, maybe a lack of grass?  Or maybe just midwinter humidity issues...


Edited by WalkingCatfish, 08 May 2019 - 05:07 PM.


#384 jkdeth

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Posted 08 May 2019 - 05:43 PM

Manure can work. Just not a requirement.

#385 WalkingCatfish

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Posted 08 May 2019 - 07:26 PM

Could be that I let the surface dry out a bit, or failed to keep the air moving. I'll change a few variables in the next round and see if I can grow tamps that don't look like worms. :biggrin:

 

In the meantime, I did get a load of stones for the old Zen garden.  If anyone knows the optimal time to harvest them, I'm curious. 



#386 el_ronhub_bird

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Posted 08 May 2019 - 08:10 PM

Just some info, although it can be used, tampenensis isn't a dung lover, doesn't require poo to fruit.

Just thought I mention that to make sure you explore other reasons for poor fruiting. Lack of poo was not the cause.


What about other stone producers? Will Atl#7 fruit off of plain straw?

#387 jkdeth

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Posted 08 May 2019 - 08:32 PM

Not familiar with a straight straw grow.

#388 Bobotrank

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Posted 10 May 2019 - 10:38 PM

Could be that I let the surface dry out a bit, or failed to keep the air moving. I'll change a few variables in the next round and see if I can grow tamps that don't look like worms. :biggrin:
 
In the meantime, I did get a load of stones for the old Zen garden.  If anyone knows the optimal time to harvest them, I'm curious.


Deleena has mentioned that in order to fruit, he will spray his casing layer quite a bit, and let it go through cycles of drying out slightly and rewetting. Humidity needs to be 98% with tons of fresh air exchange. Typically in the middle of drying out they will pin for him.

I’ve got Tamps going with Jalisco as well. Tamps have tons of stones, Jalisco not so much. Just did some transfers, and going to try and make them fruit in my Martha with computer fans blowing for 15 min every hour and humidity pumping.
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#389 WalkingCatfish

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Posted 11 May 2019 - 05:51 AM

 

Could be that I let the surface dry out a bit, or failed to keep the air moving. I'll change a few variables in the next round and see if I can grow tamps that don't look like worms. :biggrin:
 
In the meantime, I did get a load of stones for the old Zen garden.  If anyone knows the optimal time to harvest them, I'm curious.


Deleena has mentioned that in order to fruit, he will spray his casing layer quite a bit, and let it go through cycles of drying out slightly and rewetting. Humidity needs to be 98% with tons of fresh air exchange. Typically in the middle of drying out they will pin for him.

I’ve got Tamps going with Jalisco as well. Tamps have tons of stones, Jalisco not so much. Just did some transfers, and going to try and make them fruit in my Martha with computer fans blowing for 15 min every hour and humidity pumping.

 

Great information, thanks. Next time out, I'll pretend it's midsummer on the Gulf Coast, and give them periodic thunderstorms, followed by days of sweltering heat and uncomfortably high humidity. :biggrin:


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#390 WalkingCatfish

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Posted 18 May 2019 - 11:38 AM

Well, I'll be out of town for a couple of weeks, so I decided to harvest the stones. The biggest ones were a couple of inches across, the smallest about 3/8" or so. I wonder if they would have grown bigger, if I'd left them longer?

 

They look kind of delicious, and It's really hard to resist eating them all :biggrin: :

 

Stones.jpg

 

I guess I'll freeze them, rather than completely drying them. I read somewhere that, unlike fruits, they don't turn to complete mush when thawed (hope that's true!). 

 

 


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#391 WalkingCatfish

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Posted 20 May 2019 - 06:30 AM

I finally got my lazy self to read through this excellent thread.  It goes without saying that I should have done that a couple of months ago.  :biggrin:  Next time I grow these out, I'll monitor humidity levels and casing moisture a lot more closely. 



#392 Sicshroom

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Posted 14 October 2019 - 01:36 PM

So when I finish with my grows I reuse what's not harvested in my potting soil, figured it could be beneficial to the roots of what I'm growing. Well this is random but looks like its growing well enough

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#393 Soliver

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Posted 15 October 2019 - 09:34 PM

In my experience, stones freeze quite well, while drying turns them into actual, inedible stones.  I wouldn't suggest eating them all at once either  :)

 

My stone grow from the beginning of the thread went to shit.  8 of 12 jars eventually went green with no stones and the other four just .... sat.  And dried out.  No stones.  My first failure with stones, but it was a MS attempt, so back to the drawing board,

 

:)

 

soliver


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#394 newmoon

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Posted 16 October 2019 - 09:28 PM

May I play?

 

Got two boxes like this with Psilocybe tampanensis on brown rice:

resized002.jpg resized003.jpg resized004.jpg resized005.jpg resized006.jpg

 

These have been going about a month and a half. I had them somewhere too cold, so they took a while to colonize.

 

Fruited some a little while ago but the flushes were pretty bad. Took a clone of a cluster and am just now getting that off of agar.

P1020289.JPG

 


Edited by newmoon, 16 October 2019 - 09:31 PM.

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#395 ElrikEriksson

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Posted 02 November 2019 - 12:34 AM

I'm now officially in :smile:
In keeping with my tradition, 2 days after finally getting nervous all my Psi. tamp. 'Pollock' plates now show growth.
200_9253.jpg
In a few days, after these colonies send the signal to remaining spores to germinate, I can start my hunt for the isolate that produces good yields of both fruits and stones as well as fair potency so it can become my pet.

I think I will now get nervous about my eight blank 16 day old cambodginiensis plates, for luck.
I've seen mention, and even photos, of Pan. cambo. pseudosclerotia but apparently no one has even tried them?
Does coffee promote pseudosclerotia the way it does with true sclerotia?

Edited by ElrikEriksson, 02 November 2019 - 12:35 AM.

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#396 ElrikEriksson

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Posted 05 November 2019 - 12:18 AM

I'm unclear on one point.

Psi. tampanensis can both pin and stone on agar, but how does one differentiate baby stones from hyphal knots?

The few pics I've seen of baby stones on agar appear to have the stones on top of the agar as little white balls, and pics I've seen of pinning/fruiting plates have the hyphal knots as little white balls on top of the agar.

 

I should be doing my first generation of transfers tonight :biggrin:

Eight plates was certainly enough if these colonies are going to sector :laugh:


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#397 ElrikEriksson

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Posted 07 November 2019 - 01:33 AM

Another question about procedural minutiae :laugh:

I'm getting so many T1s out of these plates that I decided not to wait and to make LI out of one plate so I can do a MS grow while I do all the selecting and testing of the rest on agar. :smile:

The first step was to simmer 500g wheat, 1L half strength decaf coffee, and 1g gypsum for 31 minutes. The residuum of that being 170ml of pitch black fluid. If it were just wheat boil water I'd dilute it to 50% for agar water. If it were just unaltered 50% coffee I'd use it with a nutrient for stone producer agar.

What has been peoples experience with this stuff?

First I was thinking dilute to 50% using 50% coffee and use it for tamps. But how do non-stone producers [including gourmets] grow on this stuff?

I'm thinking my T2s should go on something more easily replicable, for science.






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