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Stone producer group grow


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#501 Juthro

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Posted 27 October 2022 - 02:04 PM

To be fair, Rocky did say agar was best practice,  not that it was the only way that will work.  It's important to remember that all spores are not created equal.  Well, not all spore prints are anyway.  A lot of the commercially available prints, and spore syringes have been made in sterile conditions, and have quite high success rates.  Though wild prints, or prints taken from open air grows aren't likely to have those same high numbers.   I don't think anyone would argue that agar does not offer better chances for success, but if you are willing to accept a higher level of contamination risk, spore to grain will work.  I've grown cubes this way back when I first started playing with mushrooms, syringe to rye berries.  Back then I was buying my syringes from Ms H, and I only ever had a couple of bags turn green, and they were due to my learning curve, and poor procedures, not dirty spores.

 

If you get a dirty syringe the only thing you're going to get out of grain is going to be failure, but with agar you can work a dirty sample, and separate out a known clean culture.   The thing is you wont know if your syringe is dirty before you try it out, and pre-sterilized bags are not cheap.  It's a real bummer when all the bags you've knocked up turn green and fuzzy.

 

IMHO it comes down to what do you personally find as an acceptable percentage of failure, vs the amount of time and effort you are willing to invest.

 

Good luck in the hobby, and good grow vibes to you.


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#502 rockyfungus

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Posted 27 October 2022 - 02:25 PM

Thank Juthro!

Spores to grain I just think is a terrible waste of resources. When we can generate so much more from proving we have a clean culture and keeping a master plate.

PF tek is the best way to get a quick thrill :) 


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#503 Juthro

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Posted 27 October 2022 - 03:46 PM

I also love the PF tek, and it is always the route I suggest for new growers.  It's also the route I generally take for growing a small personal stash once in a while.   If your looking for a big canopy of boomers it's not a good choice, but if you just want an almost foolproof method of producing a small stash of mushrooms without much hassle, it's hard to beat PF.   

 

Though you really do need a pressure canner to do PF, IMO


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#504 MushLuvR

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Posted 28 October 2022 - 05:51 PM

Well Said... @Juthro, I used to do the same back in the day with a self-made injection port from Permatex, and a Tyvek filter is where I started.  I went straight to a PC first purchase and WBS and never looked back (most don't start out on this path).  At the time, I was willing to accept failure with bird seeds being DIRT cheap 10+ years ago and a Greenhorn to the Game.  Flame sterilization and a SHIP will DEF work (successful with 90 min sterilization, and a clean flamed Spore Syringe) but riskier practice as previously mentioned (ALL Spores are DEF not created Equal, it hurts to lose 7 jars... Believe me I KNOW), and I also prefer Agar for a clean culture to start with... but many Paths to the Promise Land (ask 10 people you get 10 answers).  My advice is always stick to whatever style of growing (to the T) and don't get in your own way adding shit or thinking of "trying" this or that... DON'T step on your Richard.  My 5 mins is up... STAGE Left... I will.  


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#505 tuftygrasses

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Posted 30 November 2022 - 03:30 PM

The LC i made for these stones in hmm 2018 is still giving healthy growth on agar which is good because the spores I could find are proving stubborn.
How hard the galindoi is to survive these years and moving to a different place!
I am thinking to make invitro jars. I got fruit from these on agar that I forgot about.

I was going to have maybe an inch of grain in the jar and just neglect until it fruits again!

But apparently they need a casing layer. Would a simple thin layer of coir help? Could this be sterilised all together with the blanched grain and inoculated via SHIP?

Thanks :)

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#506 tuftygrasses

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Posted 30 November 2022 - 04:35 PM

Hey bez!

Ive been messing around with the ready brown rice bags lol
Knocked this up with gandoli, let it colonize, put it in the fridge to kinda snap it cold and stress the myc to produce the stones. This was one bag from dec 9th.

Anyony care to share potential weights and expectations?
As ive never had a chance with these until now.
I have them in the fridge for now

I also have galindoi on micro rice bags. Going very slow. Suspect it's partly low temps and partly not enough gas exchange with the one needle hole

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#507 MushLuvR

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Posted 04 January 2023 - 08:33 PM

Whaddup ya'll, been awhille... I just harvested the coffee-soaked Oats jar of Tamp sclerotia (over 6th months old), this one filled up a large pill bottle full of zesty sour goodness.  There for awhile I was micro dosing these, really smooth at .8 fresh, went to 1.2 and it was good, but just barely outside of the micro side.  No visuals or anything at that level, but definitely felt more of the body high instead of colors being vibrant, and mainly just really great for the mind.  Way more focused and overall, just a good feeling mentally. Anyway, I really enjoy these.  Super surprised these aren't more popular.   :meditate:

 

P1160158.JPG


Edited by MushLuvR, 04 January 2023 - 08:34 PM.

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#508 rockyfungus

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Posted 25 January 2023 - 10:30 AM

After a few years of working a really old mislabeled print I present bacterial mushrooms eating themselves.

mexi.jpg


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#509 tuftygrasses

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Posted 13 February 2023 - 11:08 AM

I'm putting wild temps on agar
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#510 tuftygrasses

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Posted 13 February 2023 - 02:45 PM

This is the culture I used for the stones I posted in this thread a few years back. It survived and after a clean up is back on grain

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#511 JuggaloJ

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Posted 16 February 2023 - 04:14 AM

My Whole Brown Rice doesn't break up when shaken although I've read you don't shake stone producing mycelium. Anyone have insight into this? Much appreciated whoop.


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#512 RescueU

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Posted 17 February 2023 - 01:36 PM

I can't answer that, but I also have some recently nocced WBR jars that are all showing mycelium growth! 9 jars of Tampanensis. Growing for stones mainly. Will leave them without shaking. I plan on leaving half in jars long term, half will go to shoeboxes once the stones form. 



#513 YoshiTrainer

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Posted 17 February 2023 - 08:10 PM

WBR is easy in grow bags but sticks like crazy in jars. The bigger the jar, the harder it is to shake. Your mycelium, if clean should colonize the WBR fine without shaking. Once colonized you should be able to shake it easier before you spawn.

Good luck!
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#514 MushLuvR

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Posted 18 February 2023 - 11:45 AM

You can shake tamp spawn, but it's not necessary since it's a fairly fast colonizer (10-12 days (LC) depending on environment).  Stone jars (or bags... even better) can be shot up and almost forgotten about for 4-6 months.   :meditate:


Edited by MushLuvR, 18 February 2023 - 11:46 AM.

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#515 RescueU

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Posted 01 March 2023 - 02:14 PM

First stones!
20230301_110937.jpg
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#516 JuggaloJ

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Posted 01 March 2023 - 07:39 PM

Do these look good?

 

Inoculated 2/13/23 via sporeworks ATL #7 spore syringe. I tried to make liquid culture but must have made it too rich because nothing grew in the 3 different jars.

 

100_1614.JPG 100_1613.JPG 100_1611.JPG


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#517 RescueU

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Posted 02 April 2023 - 07:09 PM

I had a mini tub with a quart of spawn catch trich immediately after casing, so I scraped the casing off and transferred to spawn to 2 flower pots in my greenhouse. The temps are hot during the day and down to the 40's at night right now. If anything there were a bunch of grape sized truffles that should continue to grow out there.


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