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Stone producer group grow


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#501 Juthro

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Posted 27 October 2022 - 02:04 PM

To be fair, Rocky did say agar was best practice,  not that it was the only way that will work.  It's important to remember that all spores are not created equal.  Well, not all spore prints are anyway.  A lot of the commercially available prints, and spore syringes have been made in sterile conditions, and have quite high success rates.  Though wild prints, or prints taken from open air grows aren't likely to have those same high numbers.   I don't think anyone would argue that agar does not offer better chances for success, but if you are willing to accept a higher level of contamination risk, spore to grain will work.  I've grown cubes this way back when I first started playing with mushrooms, syringe to rye berries.  Back then I was buying my syringes from Ms H, and I only ever had a couple of bags turn green, and they were due to my learning curve, and poor procedures, not dirty spores.

 

If you get a dirty syringe the only thing you're going to get out of grain is going to be failure, but with agar you can work a dirty sample, and separate out a known clean culture.   The thing is you wont know if your syringe is dirty before you try it out, and pre-sterilized bags are not cheap.  It's a real bummer when all the bags you've knocked up turn green and fuzzy.

 

IMHO it comes down to what do you personally find as an acceptable percentage of failure, vs the amount of time and effort you are willing to invest.

 

Good luck in the hobby, and good grow vibes to you.


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#502 rockyfungus

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Posted 27 October 2022 - 02:25 PM

Thank Juthro!

Spores to grain I just think is a terrible waste of resources. When we can generate so much more from proving we have a clean culture and keeping a master plate.

PF tek is the best way to get a quick thrill :) 


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#503 Juthro

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Posted 27 October 2022 - 03:46 PM

I also love the PF tek, and it is always the route I suggest for new growers.  It's also the route I generally take for growing a small personal stash once in a while.   If your looking for a big canopy of boomers it's not a good choice, but if you just want an almost foolproof method of producing a small stash of mushrooms without much hassle, it's hard to beat PF.   

 

Though you really do need a pressure canner to do PF, IMO


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#504 MushLuvR

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Posted 28 October 2022 - 05:51 PM

Well Said... @Juthro, I used to do the same back in the day with a self-made injection port from Permatex, and a Tyvek filter is where I started.  I went straight to a PC first purchase and WBS and never looked back (most don't start out on this path).  At the time, I was willing to accept failure with bird seeds being DIRT cheap 10+ years ago and a Greenhorn to the Game.  Flame sterilization and a SHIP will DEF work (successful with 90 min sterilization, and a clean flamed Spore Syringe) but riskier practice as previously mentioned (ALL Spores are DEF not created Equal, it hurts to lose 7 jars... Believe me I KNOW), and I also prefer Agar for a clean culture to start with... but many Paths to the Promise Land (ask 10 people you get 10 answers).  My advice is always stick to whatever style of growing (to the T) and don't get in your own way adding shit or thinking of "trying" this or that... DON'T step on your Richard.  My 5 mins is up... STAGE Left... I will.  


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#505 tuftygrasses

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Posted 30 November 2022 - 03:30 PM

The LC i made for these stones in hmm 2018 is still giving healthy growth on agar which is good because the spores I could find are proving stubborn.
How hard the galindoi is to survive these years and moving to a different place!
I am thinking to make invitro jars. I got fruit from these on agar that I forgot about.

I was going to have maybe an inch of grain in the jar and just neglect until it fruits again!

But apparently they need a casing layer. Would a simple thin layer of coir help? Could this be sterilised all together with the blanched grain and inoculated via SHIP?

Thanks :)

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#506 tuftygrasses

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Posted 30 November 2022 - 04:35 PM

Hey bez!

Ive been messing around with the ready brown rice bags lol
Knocked this up with gandoli, let it colonize, put it in the fridge to kinda snap it cold and stress the myc to produce the stones. This was one bag from dec 9th.

Anyony care to share potential weights and expectations?
As ive never had a chance with these until now.
I have them in the fridge for now

I also have galindoi on micro rice bags. Going very slow. Suspect it's partly low temps and partly not enough gas exchange with the one needle hole

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