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Stone producer group grow


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#41 onediadem

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Posted 27 July 2018 - 09:57 PM

I know! Crazy to me. But, like I said it could have just been the ones I grew.

 

post-102215-0-32551200-1532746592.jpg

 

 

 

 

 

 

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#42 Soliver

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Posted 28 July 2018 - 12:51 AM

I'm going for a grow strictly for extract purposes.

 

And science, of course   :)

 

That mad trip I had was fresh from the jar - I'm not sure how much I took (before cheap gram scales) but it was worth repeating ... except for the naked wandering part, and even that was sorta funny .. I was laughing at myself the whole time.

 

:)

 

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#43 PinkMenace

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Posted 29 July 2018 - 09:08 PM

I made agar following the proportions laid out in CatsAndBats Democratizes Agar (10g nutrient, 10g agar, 500ml water), using malt extract (DME) as my nutrients. After PCing for 30min at 15psi, I let cool and poured into plastic petri dishes in a still air box (SAB). I proceeded to inoculate 11 petri dishes with a spore print of Psilocybe mexicana var. tampanensis purchased from an online spore vendor. I then used Parafilm to seal the edges, attempting to protect against outside contams.

 

I made several mistakes along the way. The biggest one was that I let the agar cool too long and it solidified in the graduated cylinder. When I discovered this, I was already pouring and decided not to heat it back up in hot water. This may have been a mistake, but 11 plates (petri dishes) was good enough for me. I was frustrated and as a result may have been sloppier in my cleanliness. 

 

The other big red flag is that this lot of petri dishes may be totally contaminated. I didn't realize the only roll I had left was the one I actually cut into the packaging of when ordering petri dishes way back. I had sealed it with some cling wrap soon after, but we'll see... All I need is one plate to be semi clean and I'm back running.


Edited by PinkMenace, 29 July 2018 - 09:53 PM.

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#44 PsyBearknot

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Posted 31 July 2018 - 11:40 PM

No turn left unstoned

Agar - telephone brand
Copy cat ratios were used
Catativies of feed store oats were added
Bear like procedures were preformed
No pour jars were produced

My choice for agar and plates were solely based on current availability
Jelly jar size no pour malt extract agar with added oats While cooking

Thankful for the print donation
Excited to hear the vendor it came from

2A547796-3285-4F38-A68A-2D6189F5DC53.jpeg

Tampannensis

1/2 print was sent
In glove box was un bagged and unfolded foil produced one small spore print on foil



0FB38D25-E403-4434-BD52-2A9B2C5473E2.jpeg

Scraping of spores was preformed
Slicing of agar was preformed
7A36B758-CA0B-4F7C-B76D-9C4DE10A690A.jpeg

It has begun.
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#45 Da-1

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Posted 31 July 2018 - 11:53 PM

Good luck Bear

 

wishing all you stone throwers the best of luck and I am watching you`s so when I get a stone to throw I know what to look out for :bat: ,

 

got to love those catativities into agar makes so much sense  :tinfoil:

 

 

 

Regards, Da-1


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#46 Soliver

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Posted 01 August 2018 - 09:28 AM

My spore syringe should arrive any day now, then I'll be in the mix  ....

 

I'll be making some LC and then MS jars - I'm not studly enough yet to move on the agar front.

 

:)

 

soliver



#47 Da-1

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Posted 01 August 2018 - 09:36 AM

My spore syringe should arrive any day now, then I'll be in the mix  ....

 

Can`t wait to see what you produce 

 

 

I'll be making some LC and then MS jars - I'm not studly enough yet to move on the agar front.

 

from what I have seen with some of your work, like that bucket grow of yours it will be easier then making jelly Mr Soliver 

 

 

 

Regards, Da-1


Edited by Da-1, 01 August 2018 - 10:43 AM.

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#48 Soliver

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Posted 01 August 2018 - 10:35 AM

Spores came today!  P. Mex Jalisco - time to do up some LC jars - I'm out of Pcorn, so I guess I'll have to nab some tomorrow when I go into town ...

 

Huzzah!

 

:)

 

soliver


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#49 RamenShaman

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Posted 01 August 2018 - 03:13 PM

No turn left unstoned
Agar - telephone brand
Copy cat ratios were used
Catativies of feed store oats were added
Bear like procedures were preformed
No pour jars were produced
My choice for agar and plates were solely based on current availability
Jelly jar size no pour malt extract agar with added oats While cooking
Thankful for the print donation
Excited to hear the vendor it came from
attachicon.gif2A547796-3285-4F38-A68A-2D6189F5DC53.jpeg
Tampannensis
1/2 print was sent
In glove box was un bagged and unfolded foil produced one small spore print on foil
attachicon.gif0FB38D25-E403-4434-BD52-2A9B2C5473E2.jpeg
Scraping of spores was preformed
Slicing of agar was preformed
attachicon.gif7A36B758-CA0B-4F7C-B76D-9C4DE10A690A.jpeg
It has begun.


After you scraped your print do you slice the spores into the agar?

I only ask because I have always left the spores sitting on the agar surface and I’m wondering if that has something to do with my poor germination rates?

#50 PsyBearknot

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Posted 01 August 2018 - 06:43 PM

I usually cut into the agar on spores

It’s how I learned so have always done it

I would believe that would give the spore the maximum contact with the moist agar medium to where if they just land on the surface then only one side has contact
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#51 RamenShaman

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Posted 01 August 2018 - 07:47 PM

I see your logic.
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#52 PsyBearknot

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Posted 08 August 2018 - 09:10 PM

Bottoms up view
7 days

A5D555FD-4AA3-4EE6-A94D-7367B0465476.jpeg

6 out of 7 have this similar growth

DBDBC970-B179-4C57-8220-3C71634EABAE.jpeg

Jar 7 has bin discarded. Had very thi. Layer of agar and a empty spot in the middle. I think I hit the empty spot cause nothing really is growing.
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#53 Soliver

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Posted 09 August 2018 - 08:08 AM

2 LC jars (potato flakes & karo - 1 tsp each per 200mls) and 7 quarts of popcorn innoculated with multispore - P. Mex - three days ago ... I'll post pics when i see some growth  :)

 

soliver


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#54 PsyBearknot

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Posted 09 August 2018 - 10:18 AM

Partial print to trade or share

Condition of print

Original owner pink
Original print cut in haf
Sent print to Bear
Print was unpackaged in glovebox to reveal one full Print
User in glovebox seven scrapes were made
Although light there are still spores left

Ideally I would like to trade with a. VIP member for another stone producer
If in one week no trade with VIP will open up two gift to any VIP who wishes to join with this thread

All I ask is that you have the skill to transfer spore to agar and that you will participate in the thread

Edited by PsyBearknot, 09 August 2018 - 10:27 AM.

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#55 tuftygrasses

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Posted 12 August 2018 - 10:56 PM

These are my best so far. Had a few jars like this. A few more of putrid goop too :)

galindoi on spelt grain. From a spore vial from the neatherlands.

I've got some galindoi and a Mexican one who's name I don't have to hand on agar just now.

#56 tuftygrasses

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Posted 12 August 2018 - 11:00 PM

These

#57 tuftygrasses

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Posted 12 August 2018 - 11:08 PM

Once more..maybe not

#58 onediadem

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Posted 13 August 2018 - 01:12 AM

I am really looking forward to this grow. I really dig the sharing and caring the family gives here.


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#59 PinkMenace

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Posted 13 August 2018 - 09:51 PM

My plates aren't looking too hot, but we shall see. I'll try to get some pics up tomorrow. 

 

Any tips in how to photograph petri dishes?


Edited by PinkMenace, 13 August 2018 - 09:51 PM.

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#60 RamenShaman

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Posted 14 August 2018 - 12:37 AM

Not a stone producer just giving an example of a picture of a plate lol ;p


3A387F50-B8A5-427E-8EAC-720E45FD4A4B.jpeg
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