As is my habit I am starting a thread after I am several steps into a grow
As the title makes quite clear I'm growing some Pans. What Pans you ask? I am growing: Panaeolus copelandia cyanescens 'Hawaiian'
Obligatory aside regarding naming:
I'm honestly super confused as to what the proper name for the species. It seems to be a bit of a mess. mjshroomer insists that they are Copelandia cyanescens not Panaeolus. See here. But that thread is a year old and taxonomy can change super suddenly. I know PinkMenace did some sleuthing a few months back regarding proper naming but I cannot find the thread at the moment.
Wikipedia has them listed as Panaeolus cyanescens with only two references one of which is a Gaston Guzman paper WHICH is goddamn downloadable right off of the wikipage. Cannot say how nice it is to not have to chase it down separately (downside: its a scan so you can't ctrl + F the 92 page monstrosity). That paper however is from 1998 which is a million years ago as far as taxonomy is concerned at the moment (DNA sequencing has flipped the table on everything). Regardless Guzman lists:
C. cyanescens (Berk. & Broome) Singer [=Panaeolus cyanescens (Berk. &Broome) Sacc.;...
Which, I think is him sighting Singer who was sighting Berk. & Broome as the original people to name the species. Annoyingly Guzmans references section is not numbered not is a date specified in the citation sooooo I have no idea which publication of Singer he's refering to. He cites over 10 of Singer's works and none of the titles mention Panaeolus so honestly I have no fucking idea.\
Stamets also lists them as Panaeolus cyanescens with Copelandia cyanescens as synonym. He also cites Berk. & Broome but also fails to have a clear reference for them in his literature cited which leads me to believe these two are old old old mycologists and their original work is hard to get hold of.
TL;DR - Taxonomy is a mess and I have no idea. I also tried the Integrated Taxonomic Information System only to discover its terrible for mushrooms. God awful. I am disappointed.
Spore print courtesy of our own feline chiropteran who I believe told me they originally got the strain from Peacefrog (I could me misremembering that).
I already have spores to agar and three isolates taken:
Spore germination plate seen above. If anyone thinks I've neglected a choice sector by all means please speak your piece.
Why no A1? Well that's because it contaminated and I forgot to take pictures of it before I composted the contents ¯\_(ツ)_/¯
SO! The question now is: How do I proceed? Usually with cubes I generate LC from my agar isolates and then use that LC to inoculate grain. Having perused some other folks threads I know that Pans are a little more on the delicate side of things.
Peacefrog mixes manure in with grain, wedges a master, and G2Gs it from there. See here. Considering I am working in a SAB I'm not a huge fan of doing G2G (hence my preference for LC).
Now of course I wouldn't be me if I hadn't already sort of done both of these things with a previous attempt at growing the same strain that I didn't document here and tragically fell prey to Trich. I have been sitting on the LC (standard 5% corn syrup solution with a drop of kelp fertilizer) I made for that grow for some reason. For clarity: This LC was generated from the same spore print as seen above but a different scraping of it.
Can't located the masking tape I like to use for labels so I was forced to use micropore.
In addition to that G2G with the old culture I also inoculated six 1L jars of grain (just grain no added manure). Why? Well I've been teaching a friend to grow and they bailed on me super last minute when we were meant to go through how to sterilize grain. So I had to deal with it all myself (had already been set to soak the night before) and by the time I got to it the grain was a touch ferment-y. So I had six liters of sterile grain just sitting around, wasn't sure when friend would reschedule, and I had proofed the old Pan LC.
I inoculated those jars on the 22. Exactly a week ago and none of them have shown any growth. Does this match with the little proof I did? Honestly, I cannot remember. I should keep notes! Why am I so bad at keeping notes? Ugh, I am my own worst enemy. So maybe they're just being slow or maybe the fermentation produces some metabolites that are inhibiting mycelia growth. Only time shall tell I suppose.
Hopping back to what to do with my plates What are people's feels regarding LC vs wedging grain masters for Pans?