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Pan-Pan-Panaeolus


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#41 onediadem

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Posted 07 September 2018 - 10:01 PM

I sure do hope this round goes well for you. When you looked at the grain, did you squeeze it? I am curious how dry that really was because it sure did look dry.



#42 425nm

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Posted 07 September 2018 - 10:35 PM

I sure do hope this round goes well for you. When you looked at the grain, did you squeeze it? I am curious how dry that really was because it sure did look dry.

I did split a few grains and they were hydrated on the inside. It was just the exterior that was a touch on the dry side.

 

On the millet front no sign of growth on the test jars yet but I don't think that millet hydrated properly. I did a test simmer for twenty minutes with 10g of millet and the results look much better so I'm going to do up another little test jar tomorrow.


Edited by 425nm, 07 September 2018 - 10:47 PM.

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#43 425nm

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Posted 08 September 2018 - 12:28 AM

PSA: Look where you're setting your PC lid down kids. I done fuckered my PC's pressure gauge. Set it down only half way on a surface and it took a tumble. Only about two feet but that was enough.

Rocker weight starts hopping with the gauge stuck at 12PSI. Turning off the burner the gauge stays at 12PSI for way longer than it should. Rocker weight is a machined piece of solid metal bound only by physics. Me dropping it won't have affected it's performance. Ergo it's the gauge.

New one's in the mail because I don't like flying half blind however, I do know what stove settings reliably get me to specific pressures and hold at them.

Praise be the over pressure plug should this prove an unwise course of action.


Edited by 425nm, 08 September 2018 - 12:33 AM.

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#44 PolarDBN

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Posted 08 September 2018 - 05:17 AM

I sure do hope this round goes well for you. When you looked at the grain, did you squeeze it? I am curious how dry that really was because it sure did look dry.

I did split a few grains and they were hydrated on the inside. It was just the exterior that was a touch on the dry side.

On the millet front no sign of growth on the test jars yet but I don't think that millet hydrated properly. I did a test simmer for twenty minutes with 10g of millet and the results look much better so I'm going to do up another little test jar tomorrow.
Usually soak for 24 hour followed by a short boil. Drain for an hour then in the pc which solves most unwanted moisture in the jar. About 20% of seeds split when done sterilizing.

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#45 425nm

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Posted 11 September 2018 - 10:39 PM

Plate update!
IMG 20180911 120729

Despite my best efforts this image will not rotate :l Or rather it will not save after I rotate it *shrug*

From Top to Bottom A2 to A4 (A1 contaminated way back) and the germination plate to the right.

 

IMG 20180911 120843
Spore germination
 
IMG 20180911 120825
A2
 
IMG 20180911 120825
A3
[
sharedmedia=gallery:images:19402]
A4
 
My bet on A3 is still looking good as it continues to outpace A2 (despite being done at the same time). A4 is nice and symmetrical thus far?
 
IMG 20180911 121041
 
IMG 20180911 121020

After two shakes these are almost ready to go (these were generated by a G2G with a 250mL jar of grain I used to proof an old Pan LC).

AND

IMG 20180911 123022

 

My first jar of test millet is showing growth. So clearly, nothing wrong with the old LC. All the more mystery as to why those jars didn't show any grow.

How well it will do I dunno. As you can see, I didn't exactly stick the landing on hydration (compare with image below of second jar of test millet).

 

IMG 20180911 202740
 
Test millet #2. I simmered this batch for about 20 minutes. Much better hydration I think.
 
IMG 20180911 202845
 
Also I re-sterilized and re-inoculated those 6L of grain. Will they colonize this time? Who knows.

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#46 ShroomyTravellerFromAfar

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Posted 11 September 2018 - 11:24 PM

good luck this time with colonization



#47 425nm

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Posted 12 September 2018 - 01:26 AM

good luck this time with colonization

Why thank you. I hope your own mycological adventures proceed well as well. I saw something is fuzzing up over in your thread :P



#48 425nm

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Posted 22 September 2018 - 12:47 AM

Been busy! But all my proofs have come up clean!

 

IMG 20180920 135203

The new LC derived from culture A3 is good to go.

 

IMG 20180920 103534
 
IMG 20180920 135121

 

Plus my numerous jars of test millet are colonizing happily. Once their totally colonized I'll probably G2G them.

 

IMG 20180920 135056
 
Not to mention that first proof (for the old LC) I G2G'd has got me 2L of fully colonized grain. Think I'm going to do a little 4L shoebox batch with them. Could I expand them? Yes but I'm impatient and have a bunch more spawn coming down the pipe anyway.

IMG 20180920 135046

 

However it would seem Pans just do not care for fermented wheat. That's twice now and nothing. I should probably just feed it to them worms. I am tempted to G2G some P. pulmonaris spawn into those jars. If anything will make use of that grain its an oyster.


Edited by 425nm, 22 September 2018 - 01:27 AM.

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#49 onediadem

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Posted 22 September 2018 - 01:07 AM

My vote is for the Phoenix. 



#50 425nm

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Posted 22 September 2018 - 05:16 PM

My vote is for the Phoenix. 

To the oysters they go! Well half of the grain anyway ass that's all I could inoculate with the 250mL proof jar I had on hand.


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#51 425nm

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Posted 24 September 2018 - 01:49 PM

Mixed up a tiny batch of substrate (2L 50:50 coir:manure with 5% by volume gypsum) for those two fully colonized jars of wheat I have. Planning on spawning them later tonight. Would do it earlier but I am currently watching a rather inquisitive puppy and they would no doubt attempt to participate.

 

IMG 20180923 140041

Edited by 425nm, 24 September 2018 - 01:50 PM.

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#52 425nm

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Posted 25 September 2018 - 10:33 PM

Sterilized 8L of millet last night.

 

IMG 20180924 214032

 

It expanded more in the PC than I had accounted for : l Shaking those is going to be fun. Alas, tonight we inoculate!

 

IMG 20180924 215540

 

IMG 20180924 215546

 

Got those 2L's of grain spawned to the bulk substrate mentioned in the post just above.
 

IMG 20180925 115102

 

The phoenix oysters seem to be moving out into that fermented grain no problem soooo who knows. I guess oysters will colonize anything and Pans will not.


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#53 425nm

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Posted 26 September 2018 - 05:11 PM

Update shots on the petri cultures in descending order:

 

IMG 20180926 142008

 

A4 - Still fairly symmetrical but starting to seeing hyphal knots at the periphery. At least that's what they appear to be to me. Little dots of dense accumulations of hyphae/mycelia. I suppose we shall see if in time it will attempt to fruit off the plate.

 

IMG 20180926 142046

 

A3 - some of the same hyphal knots visible but not in the same abundance (seem to be relegated to a single one quadrant). What is interesting on this one but may not be visible from the photos is this isolate has quite the leading edge. The taller fluffier/denser mycelia is most easily visible but if you tilt the plate to catch the light you can see a much less dense leading edge of mycelia that is 5mm ahead of the denser mycelium.

 

IMG 20180926 142056
 
A2 - is exhibiting an interesting concentric ring of light then dense mycelium (I know there are more precise and technical ways of describing growth habits in petri culture but for the life of me I've had a hard time finding good reference material for it. If anyone knows of one please point me to it) except for one quadrant where for whatever reason it is far less pronounced.
 
IMG 20180926 145101
 
Spore germination plate (reminder that A1 was lost to contamination) - The mycelium on this plate seems to be exuding metabolites in this beautifully discrete little bubbles.

 

I'm hoping this Friday I'll have a crack at using a dissection scope so maybe I'll be able to get some nicely magnified shots. Hopefully the glass lids play nice with the magnification.


Edited by 425nm, 26 September 2018 - 05:13 PM.

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#54 425nm

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Posted 03 October 2018 - 11:39 PM

IMG 20181003 205703

 

Despite what it looks like side on it would seem the Pans are colonizing the tub. The Pan mycelium is that much more delicate than Psilocybe cubensis that it is hard to tell that they're colonizing a substrate? But if we peak through a corner.

 

IMG 20181003 205633

 


Its a little more obvious.
 
IMG 20181003 205640

 

 

IMG 20181003 212017

Sadly two of the G2G jars I did with a proof jar as gone funny on me. The contamination seems to be on the "sterile" grain not the grain I used to inoculate. This is why I don't do G2G in a SAB. To the worms with them!

 

IMG 20181003 205729

 

That however begs the question what do I do with all these tiny batches of experimental grain? Do I just get back on that G2G horse?

 

On the plus side all of my LC jars are coming up clean!

 

IMG 20181003 205458

 


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#55 PinkMenace

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Posted 04 October 2018 - 12:01 AM

You could milk and make up a syringe, if you want something other than G2G.

When I tried it I needed to inject two syringe worths before I was able to extract one since the water was just absorbed, but that was WBS and verm madr on the dry side. Im sure there are many guides.

Edited by PinkMenace, 04 October 2018 - 12:03 AM.


#56 425nm

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Posted 04 October 2018 - 01:08 AM

You could milk and make up a syringe, if you want something other than G2G.

When I tried it I needed to inject two syringe worths before I was able to extract one since the water was just absorbed, but that was WBS and verm madr on the dry side. Im sure there are many guides.

That is an excellent idea. I've never actually tried milking grain. I'll give it a whirl next week. I'll be away from my fungal children for the weekend starting tomorrow. I do hope nothing else decides to contaminate while I'm away.


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#57 425nm

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Posted 04 October 2018 - 02:06 AM

In gathering up contaminated grain to take out to the worms I discovered another G2G done the day before that's totally fine!
 

IMG 20181003 233704
 
IMG 20181003 233710

 

:l It would seem I need a system for tracking batches of grain that have been sterilized.

I also discovered that I have been dragging the good name of your neighbors dog through the mud. A family of raccoons have moved into the trees of the apartment building behind us and the cute little bastards have been digging in my gardens beds. For what reason I'm unsure. Worms? Beetles? I don't think coons go for potatoes? If they do they've been digging in the wrong spots.


Edited by 425nm, 04 October 2018 - 02:28 AM.


#58 425nm

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Posted 09 October 2018 - 01:12 AM

Finally I am home! It is lovely to be reunited my cultures. They are now shaken. Chances are good they'd have been even more far along if I had bought heating oil on time (my pot harvest is holding it up). Dealing with that tomorrow!

That single liter jar of the Pan spawn is totally colonized. Spawn it to another small bin I guess? Should I just do manure and gypsum this time?


Edited by 425nm, 09 October 2018 - 01:14 AM.


#59 onediadem

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Posted 09 October 2018 - 02:22 AM

I don't know why you are having issues with G2G in a sab. Interesting. What seems to be happening? Milking jars are much easier when you only have an inch or so of grain. I couldn't imagine trying that with a full jar. Has anyone ever tried it like that here? I'm not saying it can't be done, as I am sure it can. I would think the difficulty factor would rise to try to fight with that much grain, and for sure it would need a massive amount of water because with an inch of grain I end up using 2-3 syringe fulls of water to pull one syringe.



#60 425nm

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Posted 09 October 2018 - 04:55 PM

I don't know why you are having issues with G2G in a sab. Interesting. What seems to be happening? Milking jars are much easier when you only have an inch or so of grain. I couldn't imagine trying that with a full jar. Has anyone ever tried it like that here? I'm not saying it can't be done, as I am sure it can. I would think the difficulty factor would rise to try to fight with that much grain, and for sure it would need a massive amount of water because with an inch of grain I end up using 2-3 syringe fulls of water to pull one syringe.

 

I dunno either, I've had it work like a charm before. I see now pattern to this latest round of contamination however. I may just spawn all those small jars together to a tiny shoebox,.
Does Dia have thoughts on manure vs manure + coir for Pans?






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