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My first attempt with MHRB


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#21 coleman318

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Posted 16 October 2018 - 06:10 PM

Thank you, so 2nd pull I was able to reduce solvent down to able to the same 10-20 grams before freeze precipitation. Now with the 3rd and 4th pull I reduce the naptha really low and nothing falls out I am done right? End of the run?
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#22 Nitegazer

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Posted 16 October 2018 - 06:45 PM

Yup that's how is should go. You'll likely get less precipitating out each time. By the 4th pull, the yeild can be marginal.

 

If you can get a total yeild between 1.2-1.5% you're doing really good.


Edited by Nitegazer, 16 October 2018 - 06:46 PM.


#23 coleman318

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Posted 19 October 2018 - 02:41 PM

2nd pull done.

Yielded about 0.154 grams of off white powder
Yielded about 0.098 grams of this “crack” looking consistency that is slightly more yellow I. Appearance. Also there was so dark tanish goo I scraped up onto the razor and did not weight.

First pull yielded 0.272 grams
Second pull yielded 0.252 grams (salted for this pull)

Total so far 0.524 grams.

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#24 Norman

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Posted 19 October 2018 - 11:48 PM

Nice work.
If you evaporate your naptha down you might be surprised at how much waxy yellow material remains. Very potent and quite different from the white.
I’d do a xylene pull after you do your last naptha pull. It won’t precipitate but the jungle spice is worth a stinky evap.
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#25 coleman318

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Posted 20 October 2018 - 08:21 AM

I was curious where all that red goo was. If anyone is familiar with Gordotek A/B extraction it literally is easier than making a pb a j sandwich. That is if you adhere to all the lab safety guidelines stickied at the top of the spice vaults.

I was surprised by how clean this extraction went but I just quickly read over this:

“Mimosa tenuiflora (syn. hostilis)/Jungle Spice Analysis (last update oct 2012)

Mimosa tenuiflora is one of the most important DMT-containing plants. It is used traditionally in Northeast Brazil and has been sold for years by several ethnobotanical suppliers. For these reasons, it has been the focus of considerable research by Nexians. We have tested both the crude plant preparations as well as different products extracted using a variety of different methods.


Methodology

At first I started with TLC vs some reference standards, and this has eventually evolved to GC-MS and LC-MS testing of all samples



The main results are:

Orange text indicates novel research.
++++ indicates the main substance present
+++ indicates relatively high amounts present
++ indicates moderate amounts present
+ indicates small amounts present
- indicates trace amounts present


Mimosa tenuiflora (whole root bark):
++++ DMT
+++ Yuremamine
++ Catechol, homocatechol, resorcinol, etc. (possibly breakdown products of yuremamine and/or lignin)
+ NMT
+ 2-MTHBC
- Hordenine, N-Methyl-Phenetylamine, Dimethyl-Phenetylamine, N-Formyl-NMT, 1,2-Dimethyl-Tetrahydro-Beta-Carboline


DMT (extracted with naphtha, xylene + FASA or limonene + FASI):

++++ DMT
+ NMT
+ 2-MTHBC


DMT (extracted with xylene + evaporated or limonene/xylene/toluene + aqueous
saltings):

++++DMT
+NMT
+2MTHBC
+ 1-2-Dimethyl-Tetrahydro-Beta-Carboline.
+ 1-Methyl-Tetrahydro-Beta-Carboline
- Hordenine, N-Methyl-Phenetylamine, Dimethyl-Phenetylamine, N-Formyl-NMT


Conclusions.

These analyses confirmed that heating up or extracting Mimosa tenuiflora (and analysis by GC-MS) breaks down some of the original components found in the plant material, most notably yuremamine, which may play a role in making cold-brewed Mimosa preparations orally active without a RIMA/MAOI (Callaway 2005)

When extracting with solvents that are less-selective than naphtha, we found very small amounts of two other beta carbolines that have unknown effects. It's possible that at this concentration they do not affect the experience, but they might, at these or higher concentrations.

Some people believe that even in the small amounts found, these alkaloids do
affect the experience but we don’t yet know if this is the result of self-suggestion or not. The few psychonautic blind tests that have been conducted do not confirm any noticeable difference in activity.

To concentrate these non-DMT alkaloids, you can precipitate DMT from a solvent by the FASA/FASI method, and later wash the solvent with water and evaporate the water to retrieve the other alkaloids plus excess fumaric acid and some other plant impurities.

It is important to note that we do not know if a concentration of these other alkaloids is safe or desirable for ingestion. We also have yet to confirm whether at least part of these other alkaloids are artifacts of extraction/analysis or if they are present in the plant. Identification of main substances was done with reference standards and GC/LC-MS but some of the more obscure substances present were only based on mass spectra match in database after GC-MS testing, so should not be considered an absolute proof. I post further in this thread and elsewhere in this subforum the attached mass spectra and welcome any and all peer review.






Original post from dec 2011

Here's some more analysis ive been doing lately on extracts of Mimosa Hostilis

This is all from A/B extraction on Mimosa hostilis using lye as a base (and limonene as solvents in most of them, except 6 and 9, which are using naphtha, and 5, using xylene)

First, the samples:

J - Jungle - Xylene evap and then 2x warm naphtha pulls to supposedly remove (most) DMT
1- Jungle - Xylene evap
2- FASW (Fumaric Acid Saturated Water) crude saltings from limonene pulls
3- FASI (Fumaric Acid Saturated IPA) precipitation from limonene pulls - From pulls 2,3,4, yellow
4- FASI - From pull 1, white
5- FASA (Fumaric Acid Saturated Acetone) precipitation from xylene pulls.
6- FASI - Yellow part on the bottom of FASI crystallization, due to water in IPA
7- Freebase from naphtha, white
8- Freebase from converted FASI, white
9- Freebase from naphtha, yellow.
10- Freebase from converted FASW, brown impure


Now TLC plate:



The last two columns: Second last is some attempt to make n-oxide (just added 10% hydrogen peroxide to a bit of sample 7 dissolved in methanol and used that. I think 10% is too weak to make full conversion but clearly there was some change). Last column is DMT standard.

As you can see, samples 2,3,4,5 and 6 all have a top streak/spot, which is the fumaric acid. Sample J has some spots/streak at around same height but its clearly some different substance/s.

Most samples, except 8, have DMT and NMT spots. Sample 8 was not done from same mimosa, was from earlier batch, so hard to make conclusions regarding apparent lack of NMT. (see later GC-MS discussion for more mistery... )

Samples J, 1, 2 and 6 show some Beta carboline, others dont see to show it (again, GC-MS will show more weirdness, stay tuned)

The first funny things that show up in this plate: the supposed no-dmt jungle seems to have plenty of dmt. Also the NMT presence in nearly all samples is pretty interesting. More information below with GC-MS analysis (and NMR soon)




And for those that care: This was done on silica gel 60 F254 from Merck, eluent is Methanol:Ammonium Hydroxide 100:2.5

The measured Rfs are:

DMT - 0.4-0.44
NMT - 0.19-0.23
Bcarboline - 0.03
N-oxide (?) - 0.31-0.34

Next up: GC-MS of samples 1,2,4,6,7 , and NMR of samples J, 1 and 2”

Link to the source: https://www.dmt-nexu...g=posts&t=27724

#26 coleman318

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Posted 20 October 2018 - 10:37 AM

Nice work.
If you evaporate your naptha down you might be surprised at how much waxy yellow material remains. Very potent and quite different from the white.
I’d do a xylene pull after you do your last naptha pull. It won’t precipitate but the jungle spice is worth a stinky evap.



I get the xylene pull, do the same exact process as the naptha I just go straight to evaporation and skip freeze precipitation? If so, do I do the same thing with the remaining yellowed naptha? Or do I freeze precipitate the yellow naptha still and then evaporate solvent? I am assuming I do not pour off the solvent after freeze precipitation as we are trying to get everything out of the yellowed naptha?

#27 Norman

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Posted 20 October 2018 - 05:36 PM

Evaporate the naptha after nothing more will freeze precip.
Yes do the xylene pull the same as you would for naptha but it won’t precip at all just go straight to evaporation. There might be an emulsion to deal with but just let it sit and it should clear up.
It’s interesting to experiment with using a reverse order of solvents - starting with least selective and ending with most selective. DCM, xylene, naptha, heptane for example. You pull everything first with the least selective solvent and gradually purify the goo with more and more selective solvents. You can even do it with temperature - cold heptane is way more selective than hot.
Not really necessary but fun if you like that kind of thing.
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#28 coleman318

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Posted 20 October 2018 - 05:57 PM

Cool thank you, would that be a way to separate the dmt from the other alkaloids? And other alkaloids from other alkaloids? Or is it still rich in dmt just pulling other stuff too?

Edited by coleman318, 20 October 2018 - 05:58 PM.


#29 Norman

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Posted 20 October 2018 - 06:03 PM

Yes indeed.
Swishing cool heptane around with yellow gunk will leave you with yellower gunk and white DMT after you evaporate the heptane.
That’s an oversimplification, but you get the idea.
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#30 DiSc0Rd

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Posted 23 October 2018 - 10:40 AM

Since you mentioned GordoTek, you may find this newer video helpful:

[Direct Link]

Hello mycotopians, I had a friend who bought bark to do a rue + mimosa combo but he was reading up on it and he said the intensity was probably too much for him so he gifted me with a small amount of powdered bark.


attachicon.gif5EC25D92-4A8A-453C-9A65-1E0B7F7D8401.jpeg

I followed Gordotek on YouTube. Since I have no chemistry background and never have done anything like this ever Before, reading all the steps without basic chemistry knowledge I was bound to run into “gimme” where it’s not told because it’s basic knowledge. So following s video that uses very basic tools, pie plates, Saran Wrap, stainless steel spoon, stainless steel filter, coffee filters, cheesecloth optional plus all the solvent bases and acid.

After freeze precipitation in the freezer for 4 hours, final evaporation of solvent for two hours m. Scrape up and enjoy. I was very surprised by how white the spice is for my first attempt / I did not do a cleanup or Re-x and I don’t think I will need to.
( I waited two and s half days before doing the first pull) which from ~37 grams of mimosa my first pull was 0.25 grams I would say.)

The spice has been tested, probably 15mg dose trying a new pipe with the metal fitting at the end you heat up and touch a glass plate and vaporize it. The come up was gentle but the trip was as intense if not more than my last one less ringing in my ears but the patterns and colors were just as intense. It honestly sparked a lot of past memories on LSD from family on that thick blank notecard paper whoever is printing on that it’s always been awesome very kind and gentle even at face melting doses.

I think I am going to have to smoke some in the weed pipe on top of weed to get a couple back to back hits unless I can vaporize a large quantity in one hit with the metal dongle and glass dish.

Ps. I forgot to weight it so I had to dump out of container and some was left in on the sides and a little on the pie plate still very minimal amount though.




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#31 coleman318

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Posted 23 October 2018 - 06:20 PM

Is something wrong with my images? So they have location or something on them?

#32 coleman318

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Posted 26 October 2018 - 09:06 PM

Hey everyone I have a question on the dmt experience itself. I have smoked dmt four times in the last few months and each time was by no means large doses. I notice that I am pushed into a visual field about 40 seconds after I take the hit or two or three depending on how much vapor I was getting. Anyways my experiences I get comfortable and feel slightly overwhelmed by the visuals going on not really knowing how much farther it will go each time. Well after the initial visuals that I am noticing I find that at some point I “blackout” where I am not aware of anything that just took place after the beginning of the ride. I then come to usually feeling extremely euphoric as I get situated back into the environment. Usually am lying down on a bed or couch when doing it, is this a normal occurrence with other people? I am wondering if I should work on being more aware of the trip and trying to stay with it the whole time instead of zoning out or tapping out before moving onto higher doses?

I had a pretty heavy acid trip that I was not in the right mind state took a ten strip thinking it was weak and I remember being overwhelmed and the auditory hallucinations took off crazily and all of a sudden I felt like I was being sucked up into the computer fan and I was then sober. It’s like I felt or heard a snap or a click and everything turned off.

I don’t know if this is a normal physiological response to overstimuli on psychedelics if anyone can give me some pointers or info I would appreciate it.




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