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Quick question about Cloning and grain.

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#1 Deleena24



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Posted 29 November 2018 - 01:57 PM

I have an enormous fruit I am going to clone. First I am going to put small pieces of tissue from the middle of the fruit on agar, at least 2 plates.

I was also going to attempt a coring and shoot it into an LC.

Finally, my question. Would I be able to take a clean piece or pieces of tissue and simply drop them into a jar of sterile grain?

If this is possible that would be amazing. Please give me your thoughts.

Edited by Deleena24, 29 November 2018 - 01:57 PM.

#2 coorsmikey



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Posted 29 November 2018 - 02:29 PM

It's tricky but it can be done. I have done it quite a few times. The key word is "Clean". Increase your odds by doing a few. I prefer cloning to an LC because you only need a small fiber of mycelium and if goes sour there is less waste. With the grain it takes much longer and the race against contams is on. I've also taken clones to pieces of  sterile egg carton the added the colonized cardboard to jars of grain. Keep in mind I've prolly had just as many failures though as successful transfers. Don't put all yer eggs in one basket.

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#3 Deleena24



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Posted 29 November 2018 - 03:46 PM

If you say it is possible, I'm going to try it on 3 of my grain jars.

I have an LC ready, but I'm getting some premix in the mail today and I'm gonna make 2 more so I can do 3 jars, 3 LCs, 3 plates. Like you said, not putting my eggs in 1 basket.

The guy in cloning is 84g...plenty of material to work with lol.

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