I saw earlier in this thread, caked dunked in some water filled tupperware sort of tubs. I tried this recently and thought it was really super easy, more space efficient and seems to work.
Use the jars they birthed from. Replace the cakes, fill with water, close the lid and soak. Does anyone see any problems with doing it this way.
Cake floating in the jar
So if you just put distilled water in the Tek jar, can you use that water to start grain bags or jars? I read somewhere on here about injecting cakes for mycelium water?
The verm in the cakes would probably clog a needle if you tried to break it up in the water, but just sqirting and aspirating water over a colonized cake would get enough live myc particles in the solution to inoculate grains with. The problem with the cakes, would be if you did not use a filtered lid, and used a jar lid with holes and a verm barrier. Usually people make myc-water or 'slurry syringes' with clean agar or grains, that have been under a filter since inoccing.
Here's a good tek(link) to make brf agar-substitute, which is perfect for making these 'myc-slurries', and you don't need a PC to sterilize these. Take a lil bit of brown rice and mix it with water, then pour it in 1/4 pint or 1/2 pint jars, only about 1/4"-1/2" inch deep. Wipe the inside walls of the jar clean before adding the lid.
Use a lid with a self healing injection port and 1/4" gas exchange hole covered with micropore tape or some sort of fiter, tyvec works. You could use just one hole in the center of the lid, like 3/8" inches, stuffed with polyfil. That will act as GE and SHIP(gas exchange/self-healing injection port). If you use the 'standard' PF lids, put micropore tape over the holes before sterilizing, and re-tape after pulling the needle out while inoculating, so no unfiltered air gets in the jar.
For each jar, fold up a paper towel into a square, about 3"x 3", put them on top of the jar lids(this helps catch steam/water, keeping it from getting in), then put squares of tin foil on them and press it around the jars to seal(I put rubber bands/hair ties around them to hold the foil tight). Put them in the pc, or a pot(with a tight lid) on top of 2 layers of jar lid rings with a tin foil layer w/holes or a spacer(the one that comes with the pc), with water up to the top of the second layer of lid rings, not quite touching the jars.
If your using a pot instead, put the lid on and wrap tinfoil around the lid seam and seal it tight to trap steam and build pressure(a tiny bit at most), bring the water to a full boil, start your timer, then reduce the burner to the lowest setting that keeps it boiling. Boil or PC for 15-20 minutes, then let it cool slowly in the pot/pc overnight. Don't take the lid off until it's completely cool, as dropping the temp of the air outside the jars while the jars are still hot will cause condensation in the jars.
This works almost as well as agar, even for seeing and transferring sectors of substrains, plus it's pretty easy to see contams, not quite as easy as with agar though. The myc should grow 2 dimentionally across the surface, as long as it is the right consistency, sort of a paste, but as stiff as jello.
You can inoculate them by dropping ONE drop of well shaken spore solution in the center(or scrape/swab from a print), or with any live culture/LC, or a myc tissue sample, just as with agar. With agar the myc might be flatter and more 2-D, which makes spotting contams easier, as they are on the surface only, and also makes it easier to spot sub-strain sectors, and agar is more transparent.
To make the 'myc slurry' syringe, take a syringe of sterile water, flame the needle, wipe the injection port/lid with alcohol, stick the needle in the port and squirt it in. Take the needle out and get another syringe of sterile water(it takes 2 syringes of water to get back 1 full of myc/water). Pull the needle out and shake the jar(try not to get the lid filter wet), thyen flame the needle and stick it back in, then aspirate it a bunch of times. Then suck it back up, pull the needle out and wipe it with alcohol, then cap the needle.
You can use it right away, but it will look like just beige water at first, but after about a month you can sort of see the myc cloud in it. This can colonize a full pint jar of popcorn(any grain) in a 7-12 days, probably a quart in 10-15 days. You can use it for cakes, grains, agar, LC, etc.
Edited by Billcoz, 14 March 2019 - 06:43 PM.