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Basic spore printing process


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#1 TheArchangel

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Posted 30 November 2018 - 05:58 PM

Looking for advise on spore printing. There's so much stuff out there, mostly old stuff...some simple others a lil involved. I'm not planning on doing much spore printing but I'll need to take a print in a few days or I'll lose the chance this time.


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#2 Billcoz

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Posted 01 December 2018 - 12:02 AM

 I was apprehensive about taking prints too at first but I recently successfully germinated some spores from the first print I took, which was done sloppily, it's half colonized on agar right now with no contaminants.

 

 It's hard to take sterile spore prints, people will say they are never all that clean, the way I take a spore print is- 

 

 First, Clean the still air box, spray the air w/lysol, For each print, put a flat, clean piece of tin foil(it's rolled sterile), cut to about 4" x 4", in the sab, wipe a 1/2 pint wide mouth jar with alcohol, take a clean paper towel and fold it up so it's like 4-8 layers thick, one of each of those items for every print you plan to take, put them in the clean still air box.

 

 Take an xacto knife or pair of scissors & wipe em with alcohol, take a thumbtack(the colored ones you can hold w/your fingers) and wipe it with alcohol, then flame the tack tip, but be careful not to melt the plastic.

 

 When the tack is cool take it to where the growing mushroom is at and select the one you want to print(while it's still growing), and stick the tack into the top of the cap, you might wanna use two tacks at angles for stability, the multi-colored tacks are like a dollar and you can use different colors for each strain/species, color code em.

 

 Then grab(with gloved hand) the stipe(stem) at about 2/3 up and with the scissors, cut it off just below your fingers, keeping it upright so dust wont land/settle on it, and take it to the clean SAB.

 

 When your right by the sab, hold the tacks with the cap upside down and take the xacto-knife(you can flame it, I just wipe w/alcohol) and cut the stem as close to the gills WITHOUT TOUCHING as you can, careful not to let the stem fall over on the gills.

 

 Take the cap and flip it onto a piece of foil in the sab and cover it with an upside-down jar. Lift one side of the jar and prop it up with the folded paper towel, you want it to let a tiny bit of air in, but keep humidity from the cap in as well, that will help it to drop the spores.

 

 I leave them in the SAB for 1-2 days, then I take the jar off to let it dry for another day(in the sab), but then you can take another print with the same cap, pick it up by the tack & put it on a new piece of foil(or a new spot on the same piece, but wipe w/alcohol).

 

 Let them dry in the sab and then put them in a brand new zip-lock baggie(they're sterile) and label them, I label em with the date, the species, sub-strain, vendor or source for spores/LC, and also if it's the first print from the same cap #1, the second is #2 and so on, You can eat the cap when done printing lol.

 

 Think about this, a TON of people go out and take wild prints, they are definitely dirtier than indoor grown mushrooms, and they grow em out on agar and transfer the un-contammed myc to new plates, then take the clean part that grows and transfer again to new agar to "beat out" the contaminants,.

 

 Agar is not that complicated either, I'd say you got it.


Edited by Billcoz, 01 December 2018 - 02:45 AM.

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#3 mushit

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Posted 01 December 2018 - 12:14 PM

That is a good basic process, Billcoz.

TheArchangel, there are many printing teks in the archives.  Browse around a little and you will find lots of info.

Like this thread:

https://mycotopia.ne...9-printing-tek/


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#4 Appy

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Posted 09 December 2018 - 07:00 AM

Great write up on prints! I hope I didn't bungle the ones I did a couple months ago (I didn't use a SAB). We'll see when I finally use them D:

 

take a thumbtack(the colored ones you can hold w/your fingers) and wipe it with alcohol, then flame the tack tip, but be careful not to melt the plastic.

 

Check it out: I found T pins very useful for this purpose... you can flame them and not worry about burning yourself or melting plastic (yuk!), and they're longer than pushpins which is helpful for stubby fingers like mine. They also fit nicely under a 1/2 pint jar (I use 1.5"). T pins are super cheap and can be found in office supply stores.

 

31jqn9tKtwL._SL500_AC_SS350_.jpg

 

 

 

 


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#5 Billcoz

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Posted 10 December 2018 - 01:13 AM

 

Nice, those would work better than tacks, the tacks are really small so you gotta use two ta angles to hold big caps, the thicker the pin the better it'l hold.

 

 Hey, another way I've done spore prints is to put the cap gills down(of course) in a jar, I used 1/2 pint wide mouths, just be careful to not touch the top of the cap(where contams mighta landed) to any part of the inside of the jar, and wipe the jar out really good with 70% rubbing alcohol, or you could sterilize the empty jar in a pot or PC before hand to make sure but I don't think it's necessary, the cap ain't sterile.

 

 So put the cap in the jar, then put a lid on, the lid should be left loose to allow a little bit of air to get in for a day or two, then lift the lid up and grab the tack/pin(left in cap) and eat the cap, or dry it, or you can get a second print from it.

 

 You need to leave the cap really loose for another day, actually you could put a folded paper towel in between the lid and glass, to allow a gap for the spore print to dry, it's important it's dry, or it can contam, then tighten the lid.

 

 A great thing is that if the lid has a self-healing injection port you don't ever have to open it back up to make syringes, you can just stick the flamed needle through & shoot sterilized water in, ASPIRATE WELL TO MIX, and suck it back into the syringe.

 

 If it's a really dark print, you could get many syringes off one print, to do multiple syringes from one, flame and inject the first needle, shoot all the water into it, you can leave the needle from the first sryinge in the port, just un-screw the needle(while it's still in the port) and then screw on the next clean syringe of water & shoot that in, & aspirate, & suck it(yeah) up and repeat, capping each syringe when un-screwed-         

IMG_20181209_235447.jpg IMG_20181209_234201.jpg

 

 I didn't have any self-healing lids made, I just put the cap in the jar, then i put it in a ziplock bag left partly un-sealed for a day or two to allow some air to get in, when I go to use them I'll wipe the bag really good to disinfect/sanitize and stick the needle through and shoot the water and suck it up in the syringe.

 

 I have seen pics online of others doing similar(or exactly the same) things, so I don't take credit for this as a tek, I did this by accident at first, I was gonna use foil, & I had a jar to cover the cap and I dropped the cap in gills down, I picked it up a little by the tack & saw a faint print on the glass and thought about it, glass looks better than foil, so I set it back down, it does look fuckin cool through the glass bottom of the jar-IMG_20181210_004750.jpg

 

 It's not better than any other way of printing, it could have drawbacks, like storage, or the fact that the jar don't hold enough water to do as many syringes as you could get off the print, I guess you could add more water as you go.

 

 


Edited by Billcoz, 10 December 2018 - 01:15 AM.

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#6 Deleena24

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Posted 13 December 2018 - 07:54 PM

I like this. Think I'm going to do this with my cube species I want to preserve.

#7 AbnormalMortaL

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Posted 13 December 2018 - 08:33 PM

If you wanna extra simplify it for your first go, i place the caps directly on to aluminium foil and cover with a glass cup or bowl. Mist the inside of the glass lightly. Advice i received is learning agar to clean up the print and make agar transfers.

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#8 AbnormalMortaL

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Posted 13 December 2018 - 08:35 PM

..also let the print dry before you put in the Ziploc..lesson learned

#9 coorsmikey

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Posted 13 December 2018 - 08:46 PM

 

 

Nice, those would work better than tacks, the tacks are really small so you gotta use two ta angles to hold big caps, the thicker the pin the better it'l hold.

 

 Hey, another way I've done spore prints is to put the cap gills down(of course) in a jar, I used 1/2 pint wide mouths, just be careful to not touch the top of the cap(where contams mighta landed) to any part of the inside of the jar, and wipe the jar out really good with 70% rubbing alcohol, or you could sterilize the empty jar in a pot or PC before hand to make sure but I don't think it's necessary, the cap ain't sterile.

 

 So put the cap in the jar, then put a lid on, the lid should be left loose to allow a little bit of air to get in for a day or two, then lift the lid up and grab the tack/pin(left in cap) and eat the cap, or dry it, or you can get a second print from it.

 

 You need to leave the cap really loose for another day, actually you could put a folded paper towel in between the lid and glass, to allow a gap for the spore print to dry, it's important it's dry, or it can contam, then tighten the lid.

 

 A great thing is that if the lid has a self-healing injection port you don't ever have to open it back up to make syringes, you can just stick the flamed needle through & shoot sterilized water in, ASPIRATE WELL TO MIX, and suck it back into the syringe.

 

 If it's a really dark print, you could get many syringes off one print, to do multiple syringes from one, flame and inject the first needle, shoot all the water into it, you can leave the needle from the first sryinge in the port, just un-screw the needle(while it's still in the port) and then screw on the next clean syringe of water & shoot that in, & aspirate, & suck it(yeah) up and repeat, capping each syringe when un-screwed-         

attachicon.gifIMG_20181209_235447.jpgattachicon.gifIMG_20181209_234201.jpg

 

 I didn't have any self-healing lids made, I just put the cap in the jar, then i put it in a ziplock bag left partly un-sealed for a day or two to allow some air to get in, when I go to use them I'll wipe the bag really good to disinfect/sanitize and stick the needle through and shoot the water and suck it up in the syringe.

 

 I have seen pics online of others doing similar(or exactly the same) things, so I don't take credit for this as a tek, I did this by accident at first, I was gonna use foil, & I had a jar to cover the cap and I dropped the cap in gills down, I picked it up a little by the tack & saw a faint print on the glass and thought about it, glass looks better than foil, so I set it back down, it does look fuckin cool through the glass bottom of the jar-attachicon.gifIMG_20181210_004750.jpg

 

 It's not better than any other way of printing, it could have drawbacks, like storage, or the fact that the jar don't hold enough water to do as many syringes as you could get off the print, I guess you could add more water as you go.

 

I've seen people use the jar print tek since the days of PF. One thing I could never figure out or have I bothered to ask until now. When one does print into the jar like mentioned, How does one get the spores off of the glass and mixed in with the water to be sucked back in the syringes? It doesn't seem that using a sterile scalpel or needle would be very efficient at utilizing all the spores. I've always used baggies to make syringes. So what's the trick?



#10 Deleena24

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Posted 13 December 2018 - 10:31 PM

From what I have seen, it's the pressure of the water when you squirt the syringe that mixes up the spores. Everyone I've seen sucks up and shoots the water out several times, quickly, to break up the spores before finally getting all the water in the syringe and removing the needle from the bag.

Edited by Deleena24, 13 December 2018 - 10:33 PM.

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#11 Billcoz

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Posted 13 December 2018 - 11:31 PM

 

I've seen people use the jar print tek since the days of PF. One thing I could never figure out or have I bothered to ask until now. When one does print into the jar like mentioned, How does one get the spores off of the glass and mixed in with the water to be sucked back in the syringes? It doesn't seem that using a sterile scalpel or needle would be very efficient at utilizing all the spores. I've always used baggies to make syringes. So what's the trick?

 

 I said it in the reply but it was long as hell, so I understand, I just stick the needle of a full, sterile syringe through the SHIP or bag(I didn't have lids with ports at the time so I used ziplocks), and shoot the water in and suck it back, repeat a few times to break em all loose.

 

 To do more than one syringe you can shoot a few syringes of water in the jar to dilute it more, then suck it back for each syringe and cap.

 

 You want to make sure the jar is clean before printing, I washed em in mild bleach(rinsed well) then wiped with alcohol, you could sterilize in the PC to be sure, that would be best.

 

 With SHIP lids you can reduce contams by injecting needle, un-screwing syringe from the needle with it still through the port, then screw a new sterile syringe onto the needle(still through port) and so on, so you only pierce the port once, never opening the jar.

 

You could leave the spore-water in the jar for storage as well.

 

I would say the tin-foil method would be good for someone's first, but both are easy enough.

 

 Also I agree with someone's statement about learning to clean prints up with agar as good advice, I'm just learning agar myself, no probs so far.


Edited by Billcoz, 13 December 2018 - 11:38 PM.


#12 raymycoto

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Posted 13 December 2018 - 11:48 PM

Only slightly off topic - I've heard that spores tend to stick to the plastic syringe. I believe I saw a tech some time ago that recommended adding just a tiny bit of agar to the syringe water, like 1 gram agar per liter, to keep spores in suspension. Are sticky spores on the syringe even a problem?

 

Have you heard of this or similar solution? Of course, one would want to sterilize the dilute agar water first.

 

And, maybe a better solution to getting spores into the jar - rather than putting the cap into the jar, lay it across the jar opening suspended somehow with toothpicks or something. I would be concerned that the cap surface touching the jar might not be sterile.


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#13 AbnormalMortaL

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Posted 13 December 2018 - 11:50 PM

And how does the jar work when your trying to print monsters..I've barely fit one in a bowl?

#14 AbnormalMortaL

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Posted 13 December 2018 - 11:56 PM

4 inch cap wouldn't fit in a quart jar..i tried..hence the bowl lol

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#15 Deleena24

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Posted 14 December 2018 - 10:48 AM

Pretty sure you can cute the monster caps into like 4 pieces and get 4 partial prints that are the same size as regular caps.
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#16 AbnormalMortaL

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Posted 14 December 2018 - 11:11 AM

Cool. Thanks. I wasn't sure if they would still drop spores after being mutilated.

#17 Billcoz

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Posted 14 December 2018 - 11:26 AM

Only slightly off topic - I've heard that spores tend to stick to the plastic syringe. I believe I saw a tech some time ago that recommended adding just a tiny bit of agar to the syringe water, like 1 gram agar per liter, to keep spores in suspension. Are sticky spores on the syringe even a problem?

 

Have you heard of this or similar solution? Of course, one would want to sterilize the dilute agar water first.

 

And, maybe a better solution to getting spores into the jar - rather than putting the cap into the jar, lay it across the jar opening suspended somehow with toothpicks or something. I would be concerned that the cap surface touching the jar might not be sterile.

 Yes, I have read a tek somewhere about making a "cradle" so the cap isn't touching the glass to reduce contams and I haven't heard of agar in syringes but it sounds like someone does it so yeah, it's common for people to use a drop of water-soluble astroglide(sex lubricant) to keep spores from clumping in the syringe.

 

And how does the jar work when your trying to print monsters..I've barely fit one in a bowl?

 Lol, that's a fun problem, but you could cut it like D24 said, or if you want it whole on glass with a flat bottom, what about these-

cc9646ad-f191-48c0-8a58-d72e47294177_1.0

https://www.googlead...Q9aACCFo&adurl=


Edited by Billcoz, 14 December 2018 - 11:32 AM.


#18 AbnormalMortaL

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Posted 14 December 2018 - 01:24 PM

They're nice..but like you said before if you're strapped for space..how many of those jars can you fit in an envelope? I have over 20 prints in the same little drawer.
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#19 Billcoz

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Posted 14 December 2018 - 07:48 PM

They're nice..but like you said before if you're strapped for space..how many of those jars can you fit in an envelope? I have over 20 prints in the same little drawer.

 Well you just gotta learn how to fold glass! No lol, storage is the problem if you don't want to use em right away, and tin-foil works just as good, it's a cool way to do it sometimes though.



#20 jkdeth

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Posted 17 December 2018 - 09:41 PM

Great method for clean as possible prints:

https://mycotopia.ne...g/?fromsearch=1
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