I've read a few opinions on long term storage of a specimen. I have a specimen or two on agar I want to keep mainly to experiment with the technique but also in order to propagate again in 6 mo or so if I choose. They are right now on agar.
Another thought on preserving a specimen is that it might be a while before one gets fruit from the specimen on agar. By that time, agar specimens are getting old unless you keep transferring and then there is the question of senescence from prolonged growth.
So . . . it seems that it's best to keep the specimen cool and free of nutrients and oxygen for preservation.
Here is my plate BTW. It's a bit old and past its prime but these are monoclonal at this point and the subsequent transfer and LC from agar did well (so far).
Additional question btw. Note the dark, tall growth on the plates. I have seen this on other old plates. Is this some sort of primordia or pinning perhaps? Don't think its contamination.
What I had planned to do was to scrape some myc off the agar then insert into a small, sterile specimen cup with sterile water.
My concern is that I'll likely not get all the agar off the specimen so it will have some nutes with it in the water. But I'll use a #10 blade and just scrape it off, I guess.
Does this sound like a good plan?