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F+ Albino strain.


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#21 Cuboid

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Posted 20 December 2018 - 02:09 PM

Doesn't look like mycelium to me.
Bacteria or slime mold or yeast maybe ...
Sorry.

#22 raymycoto

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Posted 20 December 2018 - 04:48 PM

Yeah. That looks bacterial. Spores don't start off that way. What's your agar type? How did you inoculate the agar? Scraped spores? Spore water? Hydrated spores from print? Where on the plate did you place the inoculant?

 

Sorry :(



#23 pastyoureyes

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Posted 20 December 2018 - 09:18 PM

Spore water in a syringe. Placed a drop or two dead center on the agar. Agar is PDA with a small amount of ground oats.

Edited by pastyoureyes, 20 December 2018 - 09:19 PM.


#24 jkdeth

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Posted 20 December 2018 - 10:15 PM

I'm not sure there's anything growing there yet. Just the water can cause that look. I'd give it some time.
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#25 pastyoureyes

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Posted 20 December 2018 - 10:22 PM

I'm not sure there's anything growing there yet. Just the water can cause that look. I'd give it some time.


I thought that as well but couldn't remember from past agar experiences a couple years ago. It does not seem to be growing.

#26 raymycoto

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Posted 21 December 2018 - 12:34 PM

 

I'm not sure there's anything growing there yet. Just the water can cause that look. I'd give it some time.

 

Good point. If that was just spores with trace water then that would be a problem.



#27 pastyoureyes

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Posted 21 December 2018 - 06:17 PM

Here's an update on the 2 tubs spawned last weekend.

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#28 pastyoureyes

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Posted 30 December 2018 - 07:25 PM

Put one tub in fruiting conditions yesterday, the other seems to have some excess moisture and is colonizing slowly. It may have been the one with funky looking spawn, not sure but looks clean so far.

#29 pastyoureyes

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Posted 03 January 2019 - 05:15 AM

Pins.

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#30 pastyoureyes

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Posted 05 January 2019 - 11:25 PM

I have some grain jars that are not completely colonized that are pinning invitro. Im thinking about spawning and tossing the uncolonized grains. Any opinions/experience on that?

#31 coorsmikey

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Posted 06 January 2019 - 12:59 AM

Better idea than let them go to waste. Rinse away the uncolonized grain really well. If its pinning and still has areas uncolonized you can bet that something else has colonized it. Don't put all yer eggs in one basket expecting miracles. But hey you could pull it off with a good pasteurized sub. ( not recommended for newbs and lurkers) 



#32 jkdeth

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Posted 06 January 2019 - 10:57 PM

If it's a small amount and everything looks great, it will probably do fine. If its a large amount Uncolonized and you see any slimy looking spots, its probably very bacterial. Still might be fruitable, I treat it with a lot of care though.

#33 pastyoureyes

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Posted 07 January 2019 - 07:47 AM

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#34 pastyoureyes

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Posted 07 January 2019 - 09:13 AM

If it's a small amount and everything looks great, it will probably do fine. If its a large amount Uncolonized and you see any slimy looking spots, its probably very bacterial. Still might be fruitable, I treat it with a lot of care though.


Small amount of uncolonized grain. Nothing that i can identify visually as bacteria.

#35 pastyoureyes

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Posted 07 January 2019 - 09:26 AM

Just checked again and one jar has some visible bacteria.
gallery_150062_1672_823595.jpg


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The rest look like this and seem to still be colonizing...

gallery_150062_1672_401299.jpg

#36 jkdeth

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Posted 08 January 2019 - 02:39 AM

I have one looking the same. I'm still on the fence about what to do with it.

#37 pastyoureyes

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Posted 08 January 2019 - 04:10 AM

I'll probably still spawn mine but not use the bottom portion.

#38 pastyoureyes

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Posted 08 January 2019 - 05:03 AM

First flush just before harvest.
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#39 pastyoureyes

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Posted 15 January 2019 - 11:18 PM

Second flush

gallery_150062_1672_1367103.jpg
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#40 pastyoureyes

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Posted 17 January 2019 - 06:03 PM

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