First off thanks for the input guys. This is exactly what I was after, and this is the conclusion I had drawn. That with the different strains like pretty much any other population of individuals we have averages, we have a few with less, a few with more, but generally a strain overall will have an average potency, height, shape ect. For example Peurto Ricans may display a higher potency and more aggressive colonization than Golden Teachers (on average).
I have a bit of lab experience, and it really would have been irresponsible of me not too. So I shelled out and bought everything I need to perform TLC a couple of weeks ago. So I kind of have everything ready to go, I even have the visualization reagent already, will be using Ehrlichs reagent in a small spray bottle to spray on the plates and develop them. I have a good camera so I will document the process in a thread in mad scientists in the very near future, I have just put down a dozen different strains so its going to be a long month waiting. We should be able to get a good fingerprint of each individual and even each strain, including being able to tell if a shroom or strain produces baeocystine or other interesting metabolites that may be present and make strains feel stronger, or produce more body load ect. I was reading a paper (will attach it to this post, and they state;
We found that the levels of psilocybin varied somewhat unpredictably
from one flush to the next, but generally were much the same on the last
flush as they were on the first flush (Table 1). Psilocin, on the other hand,
generally was absent in the first one or two flushes, reached maximum by
the fourth flush, and then appeared to start to decline
Which if true those later flushes would definitely feel a lot stronger or more potent, as the quicker acting psilocin kicked in all at once.
Also the results should be a goldmine for a nerd like me, because the Rf values are well known combined with the Ehrlichs I should be able to analyze the metabolic pathway of the more potent shrooms to some extent.
Catfish pointed out a theoretical problem I was facing, regarding practicality. I decided I am going to go with sterilized ice cube trays with lids that I have written a number on each cube section, quarter filled with either LC or distilled water. Harvest a shroom take a tissue culture drop the culture in the ice cube tray, note which number it went into. Then place the rest of the shroom on a drying rack that has also been numbered obviously putting it into the same number as the tissue culture it came from(rinse and repeat ad nauseam). I grabbed a ton of plates(over 100) and I can get about 5 tests per plate, and can just grab more if needed. I had to get spend a little bit of $$ on equipment to make sure the results can be used to quantify also. I could really get into the nitty gritty, but I am sure I would end up boring everyone. If you would like me too though I would. After I iron out the details, it will be a pain sorting out the mobile phase and resolution(but this is what I do!). Once that is done I do not see why it could not be repeated by anyone that wanted too, easily with blotting paper or filter paper as the stationary phase instead of expensive glass plates.
I inoculated some WBS jars with a PE clone last week so that wont be too far away, and we can at least get some substrate tests done while waiting on the spores. Which substrates/additives work in the potency department ect.
VARIATION OF PSILOCYBIN AND PSILOCIN LEVELS WITH REPEATED FLUSHES (HARVESTS) OF MATURE SPOROCARPS OF PSILOCYBE CUBENSIS (EARLE) SINGER