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In Vitro grow q's


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#1 FilzShroom

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Posted 02 January 2019 - 01:03 PM

Hi guys,

 

I'm hoping someone could clear up a couple things for me with regards to invitro grows....

 

When growing invitro, do you still birth the BRF cakes and dunk them for 12-24 hours?? (I suspect no, pretty sure it only applies to 2nd, 3rd flush?)

Do any of you take the cakes out of the jar and flip it over?? or just remove the lid, empty out the dry vermiculite then leave it as is....??

(loosen the cake and flip the jar upside down)

 

One of the remaining GT went crazy and colonized the dry vermiculite, should it be cut\scrapped off??

Or should I pop that one into the SGFC aswell?

 

My current experiment consists of 6 fully colonized GT in 250 wide mouth jars

4 have been soaked, rolled and put into my SGFC 3 days ago.

2 will be used for invitro

 

6 Ecuador 70% colonized

6 Z strain 25% colonized

6 B+ 15% colonized

 

thanks in advance

 



#2 FilzShroom

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Posted 03 January 2019 - 11:36 AM

Found the Hippie's Neglect Tek so I'm good


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#3 Myc

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Posted 03 January 2019 - 11:48 AM

You're a Free Member now FilzShroom. 

I'll move this out to Magic for more views and commentary. Glad you've joined us! Welcome. 


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#4 Deleena24

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Posted 03 January 2019 - 12:56 PM

Look for Chronic Tek in the Vaults/Archives.

I think you'll really enjoy the method. Its essentially Neglect Tek, except you place the cake in a slightly bigger jar so the mushrooms have a small amount of space to grow. No misting or fanning.

This was thought up so that people could get usable prints from invitro grows, if that's something you're interested in.
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#5 FilzShroom

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Posted 06 February 2019 - 02:28 PM

Some B+ and GT65bf47963be085e2484cbe4d2151b2c0.jpg0cb3a7ee424350c6b0d9b95a4f0e95ad.jpg

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40cf006a10539ec999c46db21a20d7b9.jpg

Edited by FilzShroom, 06 February 2019 - 02:31 PM.

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#6 Deleena24

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Posted 07 February 2019 - 01:13 PM

Nice! I like seeing invitro grows. I would only make 1 small suggestion.

The cakes in the foreground aren't lying completely flat because the small height difference. That's a good thing, it allows air to flow in from the bottom.

The jars in the background don't have that small bit of space underneath to allow for air flow. I like putting them (the upside down invitro jars) on a wire cookie cooling tray so they're sitting slightly off the surface like the jar that has pins, but that's just me. They sell em in the dollar store, just FYI.

Maybe it doesnt make 1 bit of difference, maybe it does. Just food for thought.

#7 Stametsistheman

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Posted 07 February 2019 - 06:12 PM

I'm currently trying 'PF TEK improved' as described in L.G.Nicholas's book. Simply leave the cake in the jar but cover the jar all around the sides so only the top gets light meaning there will be just as many mushies but over a longer period of time. Once colonised you simply break up the verm on top and keep it moist in a moist fc and expose to light 8hrs a day.... Seems to be working well so far. 



#8 FilzShroom

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Posted 24 May 2019 - 03:14 PM

So I started a new batch.... Mazatapec.
I think I might have a couple contaminated jars....??? What do you guys say?04d1eee346505499f0b3ca41a04a815b.jpg24687f155b48a8ccc096a0f89b09e98b.jpg7cbffe7ebf27a2ddc829678ecc6d8042.jpge111991f81fb2f157562ad1c372e4acf.jpg

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#9 raymycoto

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Posted 24 May 2019 - 08:30 PM

 

I think I might have a couple contaminated jars

 

Normal metabolic waste or pigments. Those jars look good.



#10 peacefrog

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Posted 24 May 2019 - 09:42 PM

Those jars look bacterial from my couch, but the fruit should be fine.

#11 FilzShroom

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Posted 25 May 2019 - 05:44 AM

Awesome good to know thanks

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#12 raymycoto

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Posted 28 May 2019 - 10:30 AM

I defer to your opinion, PF! You have 20x more experience. Yeah, when there is an uncolonized area, or jar, etc, there is prob something else growing there.

 

Filz, let us know how they work out!


Edited by raymycoto, 28 May 2019 - 10:45 AM.


#13 FilzShroom

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Posted 04 June 2019 - 06:17 AM

Picked a couple this morning.b3180d558761b369b589e0f7f973f732.jpg2e3d28fac192f8b71a7d5f4637fc075b.jpg

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#14 raymycoto

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Posted 05 June 2019 - 06:47 PM

Nice work!

 

So you using that plastic container as your FC? And do you do anything for gas exchange?

 

Can you give a link to the 'Chronic Tek'?


Edited by raymycoto, 05 June 2019 - 06:59 PM.


#15 FilzShroom

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Posted 06 June 2019 - 06:36 AM

Yeah I'm using various sized plastic containers as my fruiting chamber. They all have holes for air Exchange but I also open them three to four times a day to spray and fan.289bdfd6a1170124c67d0c759020209a.jpgd6fafcd423d21404f97074e829717558.jpg4a323102ad227a383973089ac7f87bd6.jpg89607c47a815c68425662bc746574d3e.jpg

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#16 raymycoto

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Posted 06 June 2019 - 12:21 PM

Once again, nice!! Textbook perfect!



#17 FilzShroom

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Posted 08 June 2019 - 11:02 AM

Cubensis venom ;)5f5ce5be16d638338f00f40ba763b1dd.jpgc73c822838e9dd574119c1870d89bc5b.jpg

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#18 firerat

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Posted 08 June 2019 - 07:24 PM

Haha look at that fat bastard!!

#19 FilzShroom

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Posted 20 June 2019 - 02:17 PM

Venom and his friends :)0b12c436ec4c0bf6ae6225f60fd3fb41.jpgaac07ac19ac580971fe2539152106099.jpg6d021d773bbdc8f35b02e99ec7d1ef36.jpg2bc2895edd0cb2dbf2bb53bd0708d7ba.jpg

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