I need an opinion here. I've struggled a bit with my Pan cyan Aussie work for about 2 months. It grew nicely on PDA, a bit different from cube, of course, but although slow and different, it did seem a tad more resistant to contamination. I got a good isolate that I was happy with and made an LC from agar.
I noc'd a few things - some oat jars, a couple BRF PF cakes since I was playing with them for cubes and a couple plates to check for contaminants.
It was really slow on the oats with spots of good looking white but never taking off and it spent forever non-contam for a stalled jar but eventually I had to toss it. The agar showed what appeared to be clean growth from the LC. And . . . I was very impressed with its liking of the BRF cakes which it colonized about at fast as the cubes - impressive, since it hardly liked the oats.
So, fast forward now a month and I never did anything with the fully colonized 'Pan cake' still looking like a typical colonized PF cake in jar (was a pint) with the verm on top.
Meanwhile I've tried some Pan agar to oats jar and a more generous Pan LC to grain, still with a stall eventually.
Around Christmas, I added the Pan cake to a whole oats quart jar but poured out some of the oats in order to be conservative at my expansion ration. It was close to 1:1.5. To my surprise it developed dense spots of myc and although it never went solid white, it looked pretty happy. It was in two different shape jars, BTW. The faster one was a tall skinny 1.5 pint wide mouth and the other was a typical wide mouth quart/ Now about 2.5 weeks later one of the jars, the skinny, faster one developed some bluing of the myc and it has continued.
Now my experience with agar is that this just happens due, to, I presume, psilocin oxidation I'm guessing and it's not contamination. I've seen it on old cube plates. Well, the Pan plates were doing this earlier, like in about 3-4 weeks, I presume because of more psilocin in Pan cyan than other species.
Thus I'm hoping and guessing that my bluing grain jars, which are approaching 3 + wk of age are just oxidizing. The progress from white to blue grey was similar to the plates.
Two questions . . .
- your opinion on the bluing
- what's the next super conservative step to take my limited Pan spawn jar(s) to (mini) bulk or to fruit if even in vitro. I want to be conservative and just get a small success and take it from there.
Wider jar not yet bluing
Narrow jar with bluing
Month old Pan plate front and back
Edited by raymycoto, 13 January 2019 - 11:11 AM.