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#1 darkzen15

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Posted 07 February 2019 - 11:23 PM

Hi guys,

Brand new member here, brand new to mycology altogether in fact. Degree in biotech so no stranger to microbial work but for the most part, fungi were the enemy! 

 

Started my first BRF cake 8 days ago, bad news is I have not had ANY growth yet. Good news is that at the same time, no contams are showing, so literally no growth at all. I used some pretty old spores (3+ years), that I rehydrated in sterile water for 24 hours before inoculating. This is the only thing I can think of that would have caused this bad result. I did a full PC cycle, worked in a DIY flowhood, flamed needles etc, the works. 

 

Should I give it more time or just toss it?



#2 PsyBearknot

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Posted 08 February 2019 - 01:47 AM

Howdy and welcome.

Everything sounds solid so far. I’d keep them around for a bit longer

What strain?

#3 roc

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Posted 08 February 2019 - 08:45 AM

Welcome to 'topia!



#4 jkdeth

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Posted 08 February 2019 - 11:01 AM

Give it time. I've had older prints take 2 weeks before seeing growth.
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#5 darkzen15

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Posted 08 February 2019 - 11:25 PM

Howdy and welcome.

Everything sounds solid so far. I’d keep them around for a bit longer

What strain?


Thanks for the welcome man, it's an Ecuadorian cubensis strain. Day 10 now and still absolutely nothing.

Preparing some agar plates to see what's going on.

#6 darkzen15

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Posted 08 February 2019 - 11:26 PM

Thanks jk, I am planning on just leaving them for a few more weeks anyway. A memorial to my first failed attempt. Preparing agar plates as we speak, going to inoculate with the same spore syringe and see what comes up.
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#7 Turdly81

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Posted 09 February 2019 - 12:16 AM

I bet they are ok. Ive had some take a bit longer then that.

#8 darkzen15

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Posted 13 February 2019 - 11:04 PM

As an update, day 14 and still absolutely no growth at all. 0 contams,0 mycelium. I'm just gonna go with the idea that I have bad spores. My agar plates that i set up 5 days ago have no growth at all either after being inoculated from the same syringe.

#9 Musher365

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Posted 13 February 2019 - 11:41 PM

Do you have your jars in an incubator,At maybe 82f ?
Room temperature jars innoculated with ms can take forever to show any sign of life.
I once had B+ jars in an incubator that took almost 3 weeks to show any myc.
I don’t think I would abandon your jars just yet buddy.Id give them another week imo
Don’t give up before the miracle happens ! Lol

#10 darkzen15

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Posted 14 February 2019 - 04:07 AM

Do you have your jars in an incubator,At maybe 82f ?
Room temperature jars innoculated with ms can take forever to show any sign of life.
I once had B+ jars in an incubator that took almost 3 weeks to show any myc.
I don’t think I would abandon your jars just yet buddy.Id give them another week imo
Don’t give up before the miracle happens ! Lol


Yeh I'll keep them around as long as they remain contam free. I'll probably keep them around indefinitely as a reminder of my fuck up.

Getting new spores soon anyway so I am prepping some new jars and no pour plates

#11 darkzen15

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Posted 15 February 2019 - 01:16 AM

In an interesting turn of events, one jar is running about 1.5 degrees hotter than the other jars. Sign of life?

#12 Psil0cybin

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Posted 17 February 2019 - 05:48 PM

In an interesting turn of events, one jar is running about 1.5 degrees hotter than the other jars. Sign of life?

It is very possible. Mycelium generates heat. I wouldn't toss them yet.






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