So, recently, when the thought entered my mind I had been reviving my hobby with a dead culture in a liquid medium stored away in inconsistent fridge temperatures - for way too long - I though I might as well make a big batch now I know how to do it myself. That way I don't have to buy a lousy 25 ml vial of sterilized honey water for 25 euros, as I did when I started out.
Some time around Christmas I got a jar of 440 ml and made 400 ml of liquid culture medium by adding 16 grams of maple sirup like stuff to tapwater, about 4% in weight (a little less) and sterilized it properly. But - I was just getting back into informing myself online and just a few days after I inoculated the jar - and came upon the thing called a Self Healing Injection Port - Dang, I should have known this before!
So, got on it, I figured out a local source to get some RTV silicon and ordered a bunch of new jars and fixed them up crudely but functionally after some trial and error. So - what to do about the culture that is 1 - way too much for one jar and 2 - has no SHIJ? Improvised an SAB, got extra cleanly with stuff after I had prepared two jars with 50 ml of the same honey water mixture as I did before and simply divided the 400 ml culture into the two 500 ml jars filled with 50 ml of LC. Now I have two jars of 'selected' mycelium and spores:
I did it in such a way, that I very gently swirled the jar so only the light parts of germinated mycelium and probably spores, floated up to the top. That went into jar A on the 11th of January, and what was left in jar 0 on the bottom went into jar B on the same day (like a few seconds later, lol).
I am now working with jar A - since that was the mycelium that had germinated the quickest. I used it a day later with minimum growth visible in the liquid, but it started colonizing my grains in two to three days. Very nice. I am curious about jar B though. In the mean time it has developed a bit more but seems to be less happy and healthy than their nephews in jar A. They tend to form more like 'knot'-like structures and beef up in that way, rather than the more spread out growth of the mycelium in jar A. You might also be able to see the colour difference between the LC's - jar B is a bit darker/yellower as it probably has not used up as many sugars in the water.
Will have to see in the future what and or there are in fact any differences at all between the two. Will update on that later for sure!
Edited by RutgerHauer, 11 February 2019 - 08:22 PM.