39 replies to this topic

[XisCumming]

I recently had my first attempt at making a spore syringe... I had successfully made 3 spore prints on tin foil (i think i read somewhere it is better than an index card). however none of the spores that had fallen would come off of the tin foil... i had to take the needle off of the syringe and touch the tip of the syringe to the spore print, and draw back on the needle quickly... with pretty weak results. i made 5 spore syringes... and i swear i can see a collection of spores at the bottom of 3 needles... but they have been stuck there and wont move... i dont know i dont think the needles are going to work. so last night i sterilized an index car and made a spore print on it... its still in the process actually i was gonna give it 2 nights, i was wondering if anyone could give me some tips on successfully making a spore syringe... any idea why my spores wouldnt move... Newbie needs some help. also i had recently picked my very first flush, and i dont know it was weird mushrooms just kept growing for about a week, so i picked each individual as they matured to right about when the veil was going to tear, biggest shroom was only 20 grams fresh... also recently ate fresh shrooms for the first time ever... yea im rambling.. any ideas bout the print?

[hogwild]

well an easy way to make a spore syringe is to simply place a print in a plastic baggie, have a syringe w/ sterilized water prepared and cooled to room temp, poke that syringe into the bag and squirt some water right onto the spore print and even scrape if you need to, then suck up the water and there you have it, an easy way to make a syringe from a print.

speaking of prints... foaf wanted to let you know that paper has just enough nutrients to germinate spores and when you print on paper and make syringes its possible for them to not work properly or have a short shelf life. Id recommend using foil or tyvek if you want to avoid that hassle. Peace and good luck.

[unsane]

another easy way:You can just put the cap in a 1/2 pint jar with foil top and when print has fallen take out the cap replace foil till use.
when taking the print up i wipe foil top with alchohol and with a sterile
syringe shoot sterile water and scrape (if needed) threw the foil.

[siam_jim]

mines is like hogwild, i just scrape some off into a ziplock..seal..inject h2o, rub the bag a bit then suck it back up...make sure you alchol wipe the injection site. also make sure you have some air in the syringe so you can shake it better. also it better to have a glove box. hey hogwild congrad on the 1000 post.

peace
siam

[eternalfrost]

yup, i do the ziploc trick as well.

if you are making the print for the sole purpouse of syringes, you can just cut out the foil/paper all together. its only needed if you will be storing the print. just throw the cap right into the baggie to dry, or directly on the bottom of a canning jar. squirt in water, swish around, suck up.

[max]

The spores won't just fall off the foil, they have to be scraped off with a sterile knife.

[XisCumming]

Im feeling lazy today... is leaving the cap on the paper for like 3 days a bad thing? will it negatively affect my print/syringes ? thank you for the tips guys

[BitterApple]

I dont understand..... people go to such great lengths to realize a sterile environment in the pf jar and at innoculation time, and sterilization is critical during the colonization phase.

Yet the top of a mushroom cap fruited in a chamber cannot possibly be sterile, am I correct? So putting the cap in a ziplock bag should result in contamination the minute the bag makes contact with the top of the mushroom cap. This germ or competitor spore should be sucked into the syringe along with the mushroom spores. Can someone please explain?

[cherkropper]

The spores won't just fall off the foil, they have to be scraped off with a sterile knife.

A foaf has used Reynolds Non-stick aluminum foil and the spores tend to fall off when they dry. You have to be careful not to lose the spores before you get the foil sealed up. When you rehydrate the spore "flakes" in a baggy you will need to rub them a while to get them to break up in the water.

[anticheffy]

A foaf has used Reynolds Non-stick aluminum foil and the spores tend to fall off when they dry. You have to be careful not to lose the spores before you get the foil sealed up. When you rehydrate the spore "flakes" in a baggy you will need to rub them a while to get them to break up in the water.

this works !

[Requiemforyou]

How many ccs of water should be added to an avg size spore print to make syringes? i dunno if thats worded right but tahnks

I got another question whats the wet to dry conversion on weight if theres a link can someone post it

[Fungusaurus]

Hey man, it should be possible to make at least 2-3 10cc syringes out of a decent print.

whats the wet to dry conversion on weight

If youre talking bout cubensis the dry weight is app. 10% of wet weight

[spacecake]

You can make a lot of more syringes..at least 8-10 syringes...

But if you don't need that many ,then just use half a print !

[Requiemforyou]

let me rephrase that question how much water do i add to a jar taht has been printed so that the solution can be used for syringe innoculation
would the amount of water be 1/2 cup or so per print

And wouldnt it make sense the more spores taht are suspended in water the faster the colonization would be

[Requiemforyou]

wouldnt i be able to make a bunch of solution at once out of one or two prints and fill up another container in which i could just draw out the amount i need whenvr i need to like store the solution in a pill bottle or some such device...

[Hippie3]

yep

[Hippie3]

wouldnt it make sense the more spores taht are suspended in water the faster the colonization would be

nope
more than enough is just a waste

[Hippie3]

would the amount of water be 1/2 cup or so per print

a nice dark print about an inch, inch-and-a-half across
can be diluted into about 150-200 cc of water
yielding about 15-20 ten cc syringes

[Requiemforyou]

thx for the help hippy but i dont quite understand how more spores dosent make a faster colonization of tthe cake
isnt it logical that more seeds means more plants so in retrosepct more spores means more myclieum how does this not apply ro mushrooms?

[Hippie3]

using more seeds
does not make the corn grow faster
does it ?