39 replies to this topic

[Requiemforyou]

lol no you have a point there
however if that were how it worked then i t would make sense to say taht one injection of .25 ccs in one spot would make a cake colonize just as fast 1 cc split into four injeciton sites
so why even inject into 4 spots why not one spot with a 4th of amount normally needed?

[Hippie3]

now you're hitting the core issue
which is not the number of spores per drop
but instead it's what we call
points of inoculation.
now indeed using more points of innoculation
does, to a certain point,
speed up the process.
but that is entirely different than using massive amounts of spores in one spot eh.
one could have several points of inoculation
but still be using a low spore-density solution.
further
even more points of inoculation hits a point of diminishing returns
after which adding more does not significantly increase speed
because as i hinted at above
none of this ctually makes the mycelia grow faster.

[Requiemforyou]

ok i think i get it now its goes like it dosent matter if 5 spores germinate or 1000 if its in the same spots they r all goin to grow at the same pace in the same spot vs. say 8 injection spots that would really make it spread not if they r in the same spot but instead spread out

[Cryingblackoil]

:thumbup:

[Hippie3]

ok i think i get it now its goes like it dosent matter if 5 spores germinate or 1000 if its in the same spots they r all goin to grow at the same pace in the same spot vs. say 8 injection spots that would really make it spread not if they r in the same spot but instead spread out

yep

[scotia]

Sorry to bother you all but a while back I found a thread with a step-by-step guide complete with pics on how to make spore prints. The thread started with picking the shroom to printing it to extracting the spores into a syringe with helpful tips on how to keep the whole operation as sterile as possible. I've decided that the time has come to try and be self sufficiant in this hobby but I can't find that thread anywhere. If it helps there were pics of lots of up-turned glasses lined up with shrooms pinned down on foil inside em with a towel over the lot.

Can anyone point me in that threads direction and post me the link?

Much appreciated, peeps.

[Dank Side Of The Shroom]

http://mycotopia.net...read.php?t=5513

http://mycotopia.net...read.php?t=5513

[MLBjammer]

I don't know the exact thread you're looking for, but in both vaults there are sections devoted to prints and syringes. It's impossible without lab conditions to be completely sterile, but making a viable syringe is pretty easy--using a glovebox is, of course, your best strategy. Sorry I could not point to the exact thread. Peace.

[Lazlo]

Scotia, I know what tek you're talking about. I can't find it either. I believe PF was the author of that one. I know i've seen it here, but can't find it yet. Hold that thought for now.

[max]

Once a person grows a mushrooms, they should never have to buy another syringes of that type. Nothing in this hobby is easier than taking a spore print. Simply cut off the cap when it has opened and is dropping spores, and place on a piece of foil. The thick foil is best, not that cheap crap. Put a glass over top and leave overnight. The mushroom will do the rest of the work. When finished, just fold the foil and half and fold over the sides. Now you can store it for years or use it right away with this easy tek for making your own spore syringes or as we call them at the SpopreLab...'spore dispensers'.....

http://www.sporelab.com/winotek.htm

Good luck!

[Hippie3]

perhaps http://mycotopia.net...html?1061991123 ?

[SharkieJones]

Lately I've been using those mini ziplock bags and It seems that they are sterile. I just put the mushroom head in the top and allow it to drop spores into the bag. Then I squirt sterile water into the bag and suck up the spores.

[max]

Here's another one with pictures that somebody posted at the SporeLab Forums. It's definately worth checking out:

http://www.sporelab....opic.php?t=7674

[mason420]

The way I do it is really easy and effective. I simply wipe off a small area of the counter with alcohol, wipe both sides of a pice of heavy tinfoil with alcohol and set it on the area of the counter I just wiped. I then stick a bent paperclip into the top of the cap to act as a handle, and carefully cut the cap off the stipe with an exacto knife and set it on the foil (once the alcohol has evaporated). I then cover it with a glass or jar and leave it for a few hours.

When I come back I prep another spot on the counter with the alcohol and foil and get out a small ziplock baggie. I then carefully move the glass and cap to the next piece of foil and put the one with the print into the baggie. I fold the top of the bagie over and tape it shut and label the tape with strain and date.

To make a syringe I just wipe a spot of the baggie with alcohol and inject sterile water through that spot, scrape (part) of the print with the end of the needle and draw the water back into the syringe. The print never leaves the (relatively) sterile baggie and except for the few seconds between removal of the needle cap and sticking it in the baggie, the rest of the operation is sterile. Strictly speaking, a glovebox isn't even neccessary.

Easy as can be.

[scotia]

perhaps http://mycotopia.net...html?1061991123 ?

That's the one I was looking for, hippie. Thanks a lot. But all were very useful links, tips and advice everyone - thanks to all for your help. God, I love this place.

[Sky Rayven]

The way I do it is really easy and effective. I simply wipe off a small area of the counter with alcohol, wipe both sides of a pice of heavy tinfoil with alcohol and set it on the area of the counter I just wiped. I then stick a bent paperclip into the top of the cap to act as a handle, and carefully cut the cap off the stipe with an exacto knife and set it on the foil (once the alcohol has evaporated). I then cover it with a glass or jar and leave it for a few hours.

When I come back I prep another spot on the counter with the alcohol and foil and get out a small ziplock baggie. I then carefully move the glass and cap to the next piece of foil and put the one with the print into the baggie. I fold the top of the bagie over and tape it shut and label the tape with strain and date.

To make a syringe I just wipe a spot of the baggie with alcohol and inject sterile water through that spot, scrape (part) of the print with the end of the needle and draw the water back into the syringe. The print never leaves the (relatively) sterile baggie and except for the few seconds between removal of the needle cap and sticking it in the baggie, the rest of the operation is sterile. Strictly speaking, a glovebox isn't even neccessary.

Easy as can be.

This has got to be the best method I have seen yet. Not speaking from experience here but it is the most simple and effective tek I have seen. It just makes sense!! It doesn't seem like you have to f around with a bunch of crap. Very little handling = Sterile positive results!!!!

Keep it simple stupid is my new motto!

Thanx for this post mason420!!!

[mason420]

I shoulda mentioned, that I stick the cap from a milk carton under one edge of the glass while printing so there is a little air exchange...

I wrote this but didn't invent it. I just put together bits and pieces from all of the various teks I have read here. Props to the people who first thought of all of it.

[Sky Rayven]

Would you say that maybe placing a small plastic box that has been sterilized over the wole thing would help in preventing contaims?? Almost like a mini glove box but without the gloves? Or would that stop air exchage?

[Cryingblackoil]

"It's impossible without lab conditions to be completely sterile"

Not quite true. :) Fahts (much respect) has tons of great ideas. One of his ideas was printing without ever opening the jar, I was searching for it but I can't seem to find it :(

[Hippie3]

Here's another one with pictures that somebody posted at the SporeLab Forums. It's definately worth checking out:
http://www.sporelab....opic.php?t=7674

K so The First thing to do is Prepare your stuff and Make Sure everything is Perfectly Clean and Sterile

I usually Take Tinfoil and Fold it Over about 3 or 4 times and Cut it into perfect Squarez so that the mushroom will fill it out nicely instead of odd shaped tinfoil which isnt really appealing to the eye (who cares though eh?)

SouthPArk does so listen up!!!

So once You have Squarez cut up you wanna CLEAN them......Go buy some 99% ISO or 70% if your hurt and Give them a Good Wipe down before you print on them...(the more % the quicker it evaporates so get the 99% if your impatient like meh...) But once you Aline your Squarez in Rows or if you wanna do a Big sheet of Tinfoil thats fine but for me I like to Single the little bastards out for trading and whatnot but Just make sure the Tinfoil IS CLEAN!!! This is the most crucial Part to Prinitng and if you Drool or are a hairy bastard then it's not gonna work so Make sure it's 100% CLEAN

So once you lay out your Tinfoil you wanna Find a Container to Cover it with....Either a LArge Popcorn bowl(CLEAN) or sumthing that will cover all the caps with....and allow small amount of airflow..... You can either wedge an eraser under one side or what I do is use put the popcornContainer over 95% of the area and leave 5% over the edge of the surface.... that way nothing's gonna get blown in from one side rather it's gonna need alot of airflow to blow shit up and under it so whatever works for you but the Prints need to Breath and if it's Covered completley your wasting your time.....well Im not sure what happenz if it's completley covered but in order for them to Dry out for storage it needs Air Flow!

Anywayz... So now thay you have a CLEAN Worksurface and your tin foil your Ready to Begin Harvesting your Caps and begin to Print....

Take a Razor or Knife or whatever you've got and make a Clean straight Cut along the Stem and Try and make it straight as possible otherwise shit's gonna wobble and not stand up.....



Then Take the Same Razor and Cut a + into the Stem like so....




Now here's the Tricky Part......Take a Tac and Kinda Nudge in the back part to PRess out the Sides and kinda force the stem to make kinda table legs or sumthing.....






Then just Take that Tac(CLEAN) and poke it through the top center of the shroom and this will act as a handle for when you remove it without smudging the print and also if the shroom is wacked out and isnt normal or doesnt stand straight sumtimez i'll put it off to a side and it'lll equal out the balance but mainly for removing the cap..



Then just Do however many Caps your gonna do and Lay them out like so....



Then Wait about 12-24 hours for the Caps to Drop their Loads and once you've got a nice dark print you can then remove the caps and dry them and also give the Print a day or two to completley dry out....moisture isnt good for long term storage so make sure their 100% dry!!



Once Dried you can then proceed to packaging........Most people will just cover Tinfoil with tinfoil but ah.... the VAcume Sealer is the way!!

I'll just Seal up a Bag per normal and make Slots for Prints to fall into and Carefully Take each Print with (CLEAN HANDZ) and Slide them down into the Vacume Bag(CLEAN) And once you feel their in the right spot just Close the Sealer and Seal in a Few Directions and this is what you wanna end up with....



Then Take a Razor and Cut carefully to seperate them....




and Thats that.... LAbel It and you'll have Prints for a LOOONG LOOONG TIME.....I usually store them in Big thick books where nobody would even have a clue to look and even if they did their stuck in the middle part so unless they know the specific pages.... their outta luck...

Anyhootz... That's all 4 now.....


SouthPArk