I pressure cook my agar in a bottle and then pour plates and hour after it cools. Perhaps I'm doing it backwards.
That's the traditional and a fine way to do it. Best if you have a hood or nice SAB else you will catch various nasties since you have to leave the plates open for a while for cooling. Here's what I do. I refurb lab equipment so I an lucky to have these tools at the moment. It's for sale but I hope I don't sell my autoclave anytime soon.
Here I'm making a bacterial specific agar to track down some recurrent bugs. Use PDA or variations we have discussed for fungal culture instead of TSA.
I'm making 250 cc, so 10 gm agar mix + 250 cc water
Plates in this carrier is handy or I would wrap them in a breathable towel for the PC.
Use either an autoclave liquid cycle or make sure you cool the PC gradually (just don't vent it. let it cool on its own) to prevent boil over of superheated agar or any liquid you might be sterilizing.
Hot plates and sterile agar out of the cooker. I left the top a bit too tight on the agar bottle and it sucked down but no worries. Those saline bottles are great for agar! They are PP5 plastic and have a narrow, easy pour mouth and a good sealing screw lid.
Poured plates waiting to cool in a sterile lami flow area. In theory, you could let your agar cool in the bottle to around 50-60C then pour for quicker cooling.
BTW, seal the PP5 bottle immediately to keep sterile. To reheat, I put it into 1" of water in a large stock pot on the stove and simmer for about 15 -20 minutes until it liquifies.
Edited by raymycoto, 28 February 2019 - 09:38 PM.