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MS to Agar


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#41 Bobotrank

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Posted 08 April 2019 - 10:33 PM

I was able to find laser cut acrylic/plexi discs on Etsy that the guy can make 90mm, and I think could fit a wide mouth mason jar. Do you think those could withstand the pc tho? You’d be able to see in perfectly.

Edit: my fear is that they would degrade over time, or just flat out fail.

Edited by Bobotrank, 08 April 2019 - 10:34 PM.

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#42 jkdeth

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Posted 08 April 2019 - 10:50 PM

Its been done in the past. I don't recall the material.
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#43 OldNef

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Posted 09 April 2019 - 12:32 AM

Hi NotFadeAway and others

 

This thread was helpful to me, cheers fellas

 

I am a noob and have some MS plates looking similar and was facing a lot of the same sort of 'what step next' kinda dilemma

 

Hoping to do my first transfers tomorrow :cool: probably use the remainder of the MS plates to hit up some pints of oats if I can figure out if the plates are clean enough

 

I would take a photo for your better judgement guys (I tried like 100 times), but can't see shit through the condensation, poured them too hot was the problem I think, will do better next time lol


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#44 Billcoz

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Posted 09 April 2019 - 06:08 AM

I was able to find laser cut acrylic/plexi discs on Etsy that the guy can make 90mm, and I think could fit a wide mouth mason jar. Do you think those could withstand the pc tho? You’d be able to see in perfectly.

Edit: my fear is that they would degrade over time, or just flat out fail.

That would be cool, though IDK if that material will withstand a PC. When I am inoculating an agar dish that I plan to isolate sectors from, it is easy enough to slide the jar/dish into a baggie and tighten the ring down, but it would be nice to have a see-thru disk like you are referring to, I'd try one out to see if it survives the PC.



#45 NotFadeAway

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Posted 09 April 2019 - 09:06 AM

Here are a few of the Isolates I did from the original MS plates in order to ensure a good clean specimen. My agar was almost perfect this time with the exception of some light condensation. These plates have been since added to oat grain bags and are starting to colonize. I plan to post the picture of the final fruits of each. Also working on good clean LCs for each one. APE has tested clean 2x. The Malabar I need to give another try. Dammit Mycology is stimulating!
2d7ca5c6118b40577815d2972b7e74ce.jpgda174b49f44304445665b5da3ff6cc10.jpg4d4129e47a1466d88b55b26c808816e1.jpg
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#46 OldNef

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Posted 09 April 2019 - 10:01 AM

aww man

 

I hope my transfers are gonna look that nice

 

:cool:



#47 raymycoto

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Posted 09 April 2019 - 02:36 PM

Those look really nice! Make a couple slants to save and maybe do LC with those.


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#48 Billcoz

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Posted 09 April 2019 - 04:52 PM

Looks nice man.



#49 Bobotrank

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Posted 10 April 2019 - 12:59 AM

I was able to find laser cut acrylic/plexi discs on Etsy that the guy can make 90mm, and I think could fit a wide mouth mason jar. Do you think those could withstand the pc tho? You’d be able to see in perfectly.
Edit: my fear is that they would degrade over time, or just flat out fail.

That would be cool, though IDK if that material will withstand a PC. When I am inoculating an agar dish that I plan to isolate sectors from, it is easy enough to slide the jar/dish into a baggie and tighten the ring down, but it would be nice to have a see-thru disk like you are referring to, I'd try one out to see if it survives the PC.
I think I’ll give it a go, and report my findings. Otherwise I really like the plastic bag idea... but I’m a nerd, and nerds nerd. So let’s test some shtick.

Edit: holy fug, nice cultures NFA!

Edited by Bobotrank, 10 April 2019 - 01:01 AM.

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