After a long time of reading this site but having nothing to share, here's something new. This experiment is still in its early stages, but perhaps it will be of interest. Any replication or refinement is much appreciated.
The goal is to find a completely non-sterile method of cultivating mushrooms, based on a couple of observations:
1. Established mycelial growth is resistant to contamination
2. Some substrates are resistant to contamination, but won't directly allow germination.
The idea, then, is to germinate the fungi on a minimal amount of material, and then transfer to a resistant substrate and build up mass gradually from there.
1. Oatmeal - "old fashioned rolled oats"
3. Worm castings
4. Small capped test tubes (15ml or less) or tiny jars.
5. P. cubensis "B+" spore syringe from a commercial vendor
Nothing is sterilized or pasteurized.
Place one oat at the bottom of each tube, dry. Add one drop of liquid from the syringe (this is enough to hydrate the oat). Seal the tube and let sit at room temperature. Once the oat is mostly colonized, add a small amount of 50/50 coir/castings mix on top. Once that's colonized, go from there. Alternately, transfer the colonized oat to damp cardboard and expand that way.
After a week, 14/20 tubes have shown growth, 4/20 have grown mold, and 2/20 have grown bacteria. A picture is attached of one tube starting to colonize the "bulk" substrate. More replicates will be performed and better pictures added as time allows.
This also appears to work with Psilocybe cyanescens, transferring to cardboard after the oat is colonized.