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Every third day microdosing in rats

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#1 clumsy



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Posted 04 March 2019 - 02:17 PM

This is the first animal study of microdosing: rats were given one-tenth of the estimated hallucinogenic dose of DMT. Positive effects were noted for anxiety and depression. Negative effects were weight gain and neuronal atrophy (whoa!). I don't want my neurons atrophied!

#2 coorsmikey



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Posted 04 March 2019 - 04:34 PM

A study from the same folks say we are wise and more creative.





mushrooms are wiser and more creative

by Thomas Anderson And Rotem Petranker, The Conversation

Books such as Ayelet Waldman’s A Really Good Day and Michael Pollan’s How to Change Your Mind have drawn popular attention to the practise of ‘microdosing’ psychedelics. Credit: Shutterstock

We just ran the first ever pre-registered scientific study on the microdosing of psychedelics and found some very promising results.


We compared people who microdose —that is, who take a psychedelic substance such as LSD (lysergic acid diethylamide) or "magic" mushrooms (psilocybin) in very small quantities —with those who don't, and found that microdosers had healthier scores on key mental health and well-being measures.

Specifically, we found that microdosers scored higher on measures of wisdomopen-mindedness and creativity.

Microdosers also scored lower on measures of dysfunctional attitudes and negative emotionality, which is very promising.

Subtle changes, not hallucinations

Psychedelics microdosing can mean taking five to 20 microgramsof LSD, 0.1 – 0.3 grams of dried psilocybin-containing mushrooms or very low doses of more exotic substances, like 1P-LSDALD-52 or 4-AcO-DMT.

No matter the substance, microdosing implies a dose so low that the individual experiences only subtle changes, not hallucinations. People are not "tripping" on a microdose; they just go about their regular day, whether that means studying at school, going to work or taking care of the kids at home.

There has been no published science on whether microdosing works, but despite this, microdosing for self-enhancement and mental health has hit the media.

For example, a 2016 article in Wired magazine described young professionals in San Francisco and Silicon Valley microdosing to enhance their creativity and focus, and to gain a competitive advantage.


Ayelet Waldman attributed her increased well-being to microdosing in A Really Good Day: How Microdosing Made a Mega Difference in My Mood, My Marriage and My Life. More recently, Michael Pollan's How to Change Your Mind has further attracted mainstream attention to psychedelics.

Higher wisdom and creativity

No experimental study has evaluated psychedelic microdosing, and neither did we.

Randomized placebo-controlled trials are needed to talk definitively about the effects of microdosing. In the meantime, we investigated the experiences of people who already microdose.

Our survey investigated the relationship between microdosing psychedelics and mental health. We recruited participants online, especially from Reddit's microdosing community.

We asked our study participants about their microdosing patterns by having them fill in some questionnaires. As firm believers in Open Science, we have openly shared all our materials and you can find them here. Our findings are soon to be published in Psychopharmacology and you can access the preprint here.

We found that microdosers scored higher on "wisdom," but wisdom is a tricky thing to define. In this context, "wisdom" implies considering multiple perspectives, learning from mistakes, being in tune with emotions and people and feeling a sense of connection. Using this definition, microdosers were more "wise."

They were also more creative and open. If wisdom is tricky, creativity is even more so. In this case, creativity meant finding unusual uses for regular household objects: A brick and a knife. Microdosers came up with more useful, unusual and unique uses for these objects. This is a well-validated measure of divergent thinking, though certainly not the be-all and end-all of creativity.

Microdosers also scored lower on measures of dysfunctional attitudes and negative emotionality. What does that mean?

Well, dysfunctional attitudes and negative emotionality (aka neuroticismare bad. Dysfunctional attitudes are beliefs such as, "my value as a person depends greatly on what others think of me" or "if I ask a question, it makes me look inferior." Neither of these are true, and they are unhealthy to believe as they imply vulnerability to stress and depression.

Microdosers endorsed less of these unhealthy beliefs. Likewise, high negative emotionality means a higher likelihood of having a mental health disorder, and microdosers had lower negative emotionality.

An exciting future for clinical science

Our results are promising. As promising as they seem, we don't know whether microdosing actually caused any of these differences.

Maybe people with better mental health were more likely to experiment with microdosing, or perhaps there is some unknown cause that made people both more likely to microdose and to be creative.

At this point, we simply don't know what caused the differences between the groups —just that these differences existed. We need to run controlled lab studies to actually find out.

Our preliminary work also shows that people report downsides to microdosing. For example, some people found microdosing increased anxiety and mood-instability; increased aches, pains and gastrointestinal distress were also relatively common.

The most common drawback was that microdosing is illegal. Did we forget to mention that? Yes, psychedelics are totally illegal!

LSD and psilocybin were made illegal in the 1971 UN Convention on Psychotropic Substances and remain so today. The exact laws differ depending on where you live, and using analogue substances can sometimes be a legal grey area but, for the most part, microdosing makes you a criminal.

What we need now are controlled lab experiments—randomized placebo-controlled trials of psychedelic microdosing to test safety and efficacy. Microdosing research, alongside full-dose psychedelics, promises an exciting future for clinical science and the study of human flourishing.

Edited by coorsmikey, 04 March 2019 - 04:38 PM.

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#3 pharmer



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Posted 05 March 2019 - 02:29 PM

Pardon my skepticism but those researcher in the OP must have been doing full dosing for years if they think they can know the depressions and anxieties of rats.


Sure, they can observe but they can't know what's going on in the rat brains any better than they can know what's going on in mine when I'm offline.


But good on them for getting DMT testing approved. I wonder how many DMT studies are done per year since Strassman broke through the taboo on researching with Schedule 1 hallucinogens

Edited by pharmer, 05 March 2019 - 02:30 PM.

#4 clumsy



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Posted 05 March 2019 - 06:37 PM

A forced swim test is the standard method of evaluating antidepressants in lab animals.

#5 Deleena24



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Posted 08 March 2019 - 05:02 PM

A forced swim test is the standard method of evaluating antidepressants in lab animals.

Ok, that's kind of fucked up, and IMO the interpretation is very flawed. I'll leave it at that.
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#6 jkdeth



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Posted 15 March 2019 - 11:01 PM

Those rat tests are always flawed. Remember when the test showed rats given free choice cocaine would eat the coke till they died? Turns out that only happens when rats are isolated, and the only thing they can do hit the cocaine button. The same experiment, repeated in a more typical colony setting, with social interaction, food gathering, nest building, etc, showed the rats usually lost interest in the cocaine.
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#7 Cuboid



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Posted 16 March 2019 - 05:58 AM

@op - You aren't a rat though, I assume.
If you are a mouse the opposite is true re: neurons.
Effects of psilocybin on hippocampal neurogenesis and extinction of trace fear conditioning.
Catlow BJ1, Song S, Paredes DA, Kirstein CL, Sanchez-Ramos J.
Author information

Drugs that modulate serotonin (5-HT) synaptic concentrations impact neurogenesis and hippocampal (HPC)-dependent learning. The primary objective is to determine the extent to which psilocybin (PSOP) modulates neurogenesis and thereby affects acquisition and extinction of HPC-dependent trace fear conditioning. PSOP, the 5-HT2A agonist 25I-NBMeO and the 5-HT2A/C antagonist ketanserin were administered via an acute intraperitoneal injection to mice. Trace fear conditioning was measured as the amount of time spent immobile in the presence of the conditioned stimulus (CS, auditory tone), trace (silent interval) and post-trace interval over 10 trials. Extinction was determined by the number of trials required to resume mobility during CS, trace and post-trace when the shock was not delivered. Neurogenesis was determined by unbiased counts of cells in the dentate gyrus of the HPC birth-dated with BrdU co-expressing a neuronal marker. Mice treated with a range of doses of PSOP acquired a robust conditioned fear response. Mice injected with low doses of PSOP extinguished cued fear conditioning significantly more rapidly than high-dose PSOP or saline-treated mice. Injection of PSOP, 25I-NBMeO or ketanserin resulted in significant dose-dependent decreases in number of newborn neurons in hippocampus. At the low doses of PSOP that enhanced extinction, neurogenesis was not decreased, but rather tended toward an increase. Extinction of "fear conditioning" may be mediated by actions of the drugs at sites other than hippocampus such as the amygdala, which is known to mediate the perception of fear. Another caveat is that PSOP is not purely selective for 5-HT2A receptors. PSOP facilitates extinction of the classically conditioned fear response, and this, and similar agents, should be explored as potential treatments for post-traumatic stress disorder and related conditions.


Edited by Cuboid, 16 March 2019 - 05:59 AM.

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