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LC Culture: Corn Syrup vs LC mix


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#1 Mycogirl

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Posted 23 March 2019 - 10:39 AM

Can someone please tell me which one is better making LC with corn syrup or the LC Mix???

Edited by Mycogirl, 23 March 2019 - 10:46 AM.

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#2 onediadem

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Posted 23 March 2019 - 07:21 PM

I made lc's with karo for years. Now I make my own mix. Either is fine. Just make sure to not overdo your sugar content.


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#3 FesterAddams

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Posted 28 April 2019 - 02:21 PM

I found out the hard way that dark Karo has sodium Benzoate... I tried a tablespoon in two pint jars with grain and so far no signs of growth at two weeks. Still hoping for something though...
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#4 ilikethings

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Posted 09 May 2019 - 06:50 PM

I've only used Karo (clear) once.  It worked well but agitation was needed to induce continued myc growth--otherwise they seemed to stall out with a single cloud that occupied just ~5% of jar.  And I kept one jar with myc in it for almost a year and just recently (2 months ago) noc'ed up some jars with it.  From what I recall, the original Karo LC worked okay when I originally used it (~1year ago) but I ended up pitching the latest jars (I only used it as a test bc I was surprised that it still looked okay after such a long time, i.e. it was still a nice puffy cloud consistency...but did have a few black specks which I cannot explain).  And even in the past, I would have jars colonize very fast from it but for some reason only had about a 50% fruiting rate.  Lastly, as noted above, use the clear karo.  Spore prints on the other hand--i've had good luck with.


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#5 onediadem

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Posted 10 May 2019 - 12:31 AM

if the old archives come back online, I have a link for you.


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#6 Seeker2be

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Posted 10 May 2019 - 12:57 AM

Recent compilation from many sources:  (More than you ever wanted to know.)

 

Liquid cultures

(Extra) Light Malt Extract 

(Extra) Light Malt Extract (dried) & use that. 1g per 600mL of water or .667g per 400mL of water or

1 tablespoon of LME to one quart water

 Another Recipe is 500 ml of non chlorinated undistilled water 20 ml of light corn syrup Pressure cook for 45 min ...

 

Recipes may need to be scaled up or down based on the size jar you are using.  The jar size that the recipe is measured for will be in parenthesis.  These are just a few of the more common recipes available.

1000 ml water + 1g light malt dextrose powder (quart)

250ml water +  1 tablespoon honey (clear honey, not the cloudy kind)(half pint)
100ml water + 4ml light karo (quarter pint)

 

For Honey Liquid culture - 1tsp.(10g aaprox) Honey to 1 Cup(250ml) water
For Karo Liquid culture - 1tsp.(10g aaprox) Karo to 1 Cup(250ml) water

For Malt Liquid culture - 1tb.(10g) Dry Malt or Dextrose to 1 Cup(250ml) water

Another  More LC recipes:

3 tsp karo

½ tsp light malt

500 cc water

  Or just 0.6g of ME( for every 100mL of DISTILLED water

 Or just simply Grain water 1:4 dilution

  Or LC jars (potato flakes & karo - 1 tsp each per 200mls)

 Lazlo's Vanilla extract tek:3 tbs of vanilla extract in 500 cc.  "No need to worry about the alcohol in the extract . It will be cooked out after PCing".

 

Stamets' formula for "Wood Decomposers"  LC is

  • 1 L water
  • 40 gm Light malt extract')
  • 3-5gm hardwood sawdust
  • 2 gm nutrit yeast
  • 1 gm Ca sulfate

Edited by Seeker2be, 10 May 2019 - 01:22 AM.

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#7 Shadowlord

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Posted 27 June 2019 - 10:15 PM

Glad I saw this thread and stopped by!

Great to see you Seeker ☺️

Was wanting to bone back up on making LCs and there it is.
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#8 onediadem

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Posted 28 June 2019 - 02:44 AM

As promised.. By Workman

 

 

 

Quote: 
The impurities are likely adding to your success with pure sugar solutions. Mycelium needs trace elements and a nitrogen source to thrive, which are sorely lacking in carbon rich sugar solutions. I am suprised that there are any successes using pure dextrose or even relatively pure corn syrup. Also the sugar to water ratios are a bit high and can cause osmotic stress to the mycelium cells which will slow growth. 

A few years ago I tested a series of dilutions of potato dextrose broth to see what the dilution was for best growth. I started with the below stock solution. 

Stock solution (100%) 
250ml potato water from boiled potatoes 
1 tsp dextrose powder from the brew store 

I then did rough serial dilutions of 50%, 25%, 12% and 6% as shown in the image. The blue things are magnetic stir bars. The jars are illuminated from the bottom to show detail. 


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17034.jpg 


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Quote: 
Suprisingly the 25% dilution was the thickest and fastest growing, but even the minimal dilution of 6% had good growth. More than enough to be used for innoculation purposes. It might even be better since the thinner mycelium is easier to draw up into a syringe without clogging and doesn't form the thick mat on the surface. The 25% eventually formed mushrooms on the floating mycelial mat, but I didn't take a picture 

Currently I use a teaspoon of malt per 500 ml of water in a quart jar. Its a bit thick when colonized (like jello) but its an easy measure and it works fine for my purposes. Malt, like potato water is unrefined and contains all necessary elements for healthy fungal growth. It is no accident that you don't see recipes for honey water, dextrose or karo agars.


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#9 crazy1

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Posted 28 June 2019 - 06:33 AM

If workman says it............. He's done some amazing stuff.

 

People seem to want to complicate or "better" what's been done and proven so many times. There are many recipes for LC's, but it is the most simple that do the best.

 

Thanks for that post One


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