Today marks the 2 week point on my PF TEK run of Golden Teacher. I have 10 half pint jars going, all of which have good levels of colonization. 6 of them colonized at all four injection sites, one of them only 2 so far.
I've noticed in pictures on here, as well as my own grow, that the bottom of the jar does not seem to grow mycelia, so I got to thinking about why that may be. Since I've got so many jars growing, I have room for some experimentation.
My first thought is that we put these nice little holes in the lid to facilitate air exchange and leave the jar standing upright. CO2 is heavier than air, so it stands to reason that the bottom of the jar would have a high CO2 content inhibiting mycelial growth. By way of simple experiment, I've decided to try inverting one jar (the one in the foreground of the attached photo) to see if that bottom area may get filled in. I can't imagine that nobody would have tried this before, but I haven't stumbled over such a discussion after a great deal of searching here. Since I'm only inverting this one jar, I will still have the other 9 to use as a control to compare if there is any significant difference. I'll update this in a couple of days. I'm probably about a week away from birthing the most advanced of the cakes, it will be interesting to see if this will be one of them.