Have had this going on for a while and was a bit reluctant to get a new topic going to do another log, but since my last one failed I wanted to share and ask guidance when needed. Hope you are willing to share your experiences and choices in your grows here.
I used a Cambodian strain, MS vial to LC, and used 2ml of that on each of 6 220ml jars of popcorn. Almost double the LC I use normally, considering the small jars! Had to shake up twice after inoculation to redistribute all that honey water but that did spread the LC so much another shake was unnessasary, I think this is beinificial because you are breaking things up again at G2G. It colonized in two weeks after which I did the G2G on the 12th of April.
The G2G went alright, but have to manage my SAB space more efficiently in the future!
Since I had used 1 400gram bag of dry popcorn for the 6 small jars, and I know I would need 4 of those bags total to make 12 pint jars, I used the other 3 bags of popcorn I had waiting to prepare the 12 jars for sterilization and then the transfer.
The idea was that I spread one jar of generation 1 spawn to two second generation pint jars.
So, jar A went into 1 + 2, B into 2+3, .. F into 11+12.
I mixed up E and F though, because of that spatial problem in my SAB. No problem, I think.
I originally thought it would be a good idea to do a big propagator. But, since I had to toss my first one I thought it would be best to do two trays inside that propagator like previous times when I did have flushes. But, since I have so much spawn and if I want to do two trays, I don't have much room left in those trays for bulk sub.
I was thinking to do a REZ effect with a casing of vermiculite on top. I think a casing might be a good idea in a less than ideal growing environment, which is my situation at the moment. The two trays can be isolated in two separate propagators, which might reduce chances of contamination if proper care is given. I plan to tape up the holes of the propagator which micropore to keep the bugs out, since I have read that that might be a problem considering verticillium.
The two trays I'm using are a little over 3 liters and are fairly shallow, they are oven trays. I'll probably have about 4.8 liters of spawn, maybe a little less. 2.4 liters each leaves only 0.6 liters for something else.. Because of that I am not sure about REZ either, maybe just straight corn and a casing.. The sub would be just about two inches.
Well, that is all for now, hope to bring good mushy vibes your way soon!
The weather has been kind to us in the Low Lands, temperatures from 20 up to 25 Celsius these days, which is comfortable for me and the mycelium.
Edited by RutgerHauer, 20 April 2019 - 09:09 AM.