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Sectoring / Isolating

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#21 MsBehavin420



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Posted 02 June 2019 - 08:26 AM

That seems to be the consensus. Man I was hoping for some more science fun

#22 onediadem


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Posted 02 June 2019 - 03:34 PM

I pour my agar pretty thick, at least half the plate so I can shave off the sections I want. I do that so when I do go to grain, it seems the thicker the agar, the less likely it is to stick to the jar.


To answer about storage, I always make up at least twenty plates of the final transfer so I have plenty to work with for a very long time. Then I transfer into sterile distilled vials of water. When you get to that point, make sure every plate is pristine because your going to agitate the top of the myc with a sterile needle filled with the water, squirt some water onto the plate and keep doing that until you have enough in the syringe to transfer into the vial. Repeat for however many you want to store. I keep them in the box they came in in a baggie, stored in the fridge. I have no idea how everyone else does it, and they can chime in also but that is how I do it. When I run out I start over.

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#23 peacefrog


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Posted Today, 04:19 AM

I moved this thread out of the welcome mat to here. It has some good info in here.

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