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Tabby's B+ Invitro Bag


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#1 TabbyBoy

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Posted 24 May 2019 - 10:59 AM

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Yesterday I nocc'd up 2 1 lb rye grain bags with 5 cc's of B+.

I plan on doing a 1:1 ratio with the sub due to people saying that a 1:1 ratio is a lot quicker than a 1:2. (Anybody experienced in the difference?)

I have them incubating with a heater on 75 (Should i go higher?)

I plan to do Invitro because i don't wanna get trich this time or worry about it drying up like last time. Anyone have experience with bag invitro with B+?


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#2 Moonless

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Posted 24 May 2019 - 03:06 PM

Hey Tabby,

 

My first grow I used these types of bag to fruit in. If I recall correctly I had 1 lb of rye to approx. 2.5 lbs of manure and It worked swellingly, enough for a few good flushes like 4-5 maybe. This time I used one jar brown rice and one jar of manure and it works the same, can't see any differences. I am on the first flush of this grow so I will note how many flushes this time around!

 

I have heard that 75 is an optimal temperature however I have never tested it. My colonization space gets between 75-85 degrees often (No air conditioning) in the day and 60-65 at night so 75 seems a good middle ground.

 

Good luck


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#3 roc

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Posted 24 May 2019 - 03:20 PM

Keep in mind that colonizing raises the temperature inside of things up to 5 degrees.

 

I'm not big on external heat unless it's cold where you are.

 

These will perform just fine anywhere from 65-75 degrees.


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#4 TabbyBoy

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Posted 24 May 2019 - 06:25 PM

Keep in mind that colonizing raises the temperature inside of things up to 5 degrees.

 

I'm not big on external heat unless it's cold where you are.

 

These will perform just fine anywhere from 65-75 degrees.

Good to know. My house gets air conditioned due to the hot summer weather so i keep the heater in the incubation space just incase it gets too low. 

Should i lower my temp?



#5 el_ronhub_bird

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Posted 24 May 2019 - 07:18 PM

I find 75 to be pretty optimal. Fast and healthy growth at that temperature
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#6 roc

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Posted 26 May 2019 - 12:23 PM

70 - 75


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#7 TabbyBoy

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Posted 26 May 2019 - 08:17 PM

Do y'all think you could estimate how many flushes+  What the yield could be on these two 1:1 BAGS?

 

EDIT: I changed the temp on the heater to 71 since contams are more likely to happen at higher temps... Also since roc said 70-75 i figure 71 is optimal considering the 3-5 degree internal heat. Dont worry, I didn't bother the bags


Edited by TabbyBoy, 27 May 2019 - 01:12 AM.


#8 TabbyBoy

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Posted 31 May 2019 - 12:17 PM

61926529_1159643630895931_90802234612336  61353250_1026355001087430_171330130235351 week check. bag #1 looks more germinated than bag #2. even though They only got inoculated 30 seconds after each other! Does this usually happen with multiple bags?

the other side of bag #2 has some germination as well, but i don't think i need to take more than one pic. I'll check again in a week


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#9 roc

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Posted 31 May 2019 - 02:19 PM

Everything  is looking great!

Give em a week and we'll see how close you are to crumbling and mixing.


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#10 TabbyBoy

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Posted 06 June 2019 - 02:49 PM

That penis envy outside never did anything as i expected, the bugs took over and ate the hell out of it! I guess my hole also wasn't flat enough and it somehow completely split in half. gonna cover it with dirt today and say goodbye to the old love hate relationship! It wasn't meant to be i guess.... my fault entirely for not following a tek. I wont post a pic, it's the nastiest cake of mycelium I've ever seen. I will be checking my B+ bags tomorrow, gonna play it real safe with these, can't afford to have another mess up. Send good vibes to these bags! Lord knows in my shitty care they'll need it!


Edited by TabbyBoy, 06 June 2019 - 02:50 PM.

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#11 TabbyBoy

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Posted 07 June 2019 - 10:41 AM

Week 2! Very happy with the results. How close am i to mixing??

62351879_532160730649524_391320521088486Bag #162171005_465245090947437_609907131516190Bag#1

62488144_334727893836565_825547872408698Bag #2 62134778_535734316831536_713835465090583Bag #2

bag 1 has reached around the back and crawled up the front as well. Kind of like my old bag, Bag#2 has colonized real close to the inoculation point, and hasn't reached around to the back just yet.

How's it looking, How much longer?


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#12 roc

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Posted 07 June 2019 - 10:45 AM

Congratulations!

 

I'd go ahead and crumble and mix well. hold the bag and level the grain on top.

In 3-4 days you'll see growth throughout the bags.


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#13 LegoMyego

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Posted 07 June 2019 - 01:41 PM

Looking great Tabby, happy to see you went with B+! Please do as Roc said and mix those up really well now.  I'd say you'll be fully colonized within 2 weeks.  I've had bags stall out around 90% so I mix a second time.   

 

As for the 1:1, I'm sure it would work.  I went with a 1:2.5 ratio and one with a 1:4 ratio.  It just takes slightly longer to colonize.  I assume more substrate gives you more flushes, but I'm not sure there.  The pros should have some good advice there.  

 

When you're ready to mix the grain and substrate, cover it with a towel after mixing to increase CO2 (so mycelium spreads faster) and don't look at it for 5 days.  Look again and it should all be fully colonized and ready to begin fruiting conditions.  


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#14 TabbyBoy

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Posted 07 June 2019 - 03:05 PM

Alright y'all thanks a lot, I've mixed up to where you could barely tell it was ever colonized, and leveled out the top. 

So I should check in 4 days? 


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#15 LegoMyego

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Posted 07 June 2019 - 05:51 PM

You can.  I'd say give it 7 days.  It should be done colonizing 7-14 days.   


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#16 TabbyBoy

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Posted 07 June 2019 - 05:53 PM

You can.  I'd say give it 7 days.  It should be done colonizing 7-14 days.   

Thanks. Do you mean the grain alone or with the sub included? 

Rn im only waiting on the grain



#17 crazy1

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Posted 08 June 2019 - 05:17 AM

As for the 1:1, I'm sure it would work.  I went with a 1:2.5 ratio and one with a 1:4 ratio.  It just takes slightly longer to colonize.  I assume more substrate gives you more flushes, but I'm not sure there.  The pros should have some good advice there.  

 

More substrate gives you more mushrooms per flush as there is more space and nutrients. As to more flushes, that's not a set rule in anything, as there are so many variables. 

 

He meant for the grain to finish colonizing in the 7-14 days. 

 

Looking great though!!! 

 

Peace


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#18 LegoMyego

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Posted 08 June 2019 - 11:07 AM

7 days to check the rye grain again.  Once 100% colonized, break it up and mix it with the substrate.  Then cover with a towel and leave it for 5 days.  Check and it should be colonized fully.  If you are using a casing layer, add it and cover again for 3 days.  When you see a little mycelium poking out of the casing, that's when you start fruiting.  

 

100% colonized rye grain

 

Colonized substrate

 

3 days after adding casing.  At this point, I removed towel fully, began fanning everyday and misting when necessary. 

 

 

 


Edited by LegoMyego, 08 June 2019 - 11:15 AM.

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#19 TabbyBoy

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Posted 08 June 2019 - 04:35 PM

7 days to check the rye grain again.  Once 100% colonized, break it up and mix it with the substrate.  Then cover with a towel and leave it for 5 days.  Check and it should be colonized fully.  If you are using a casing layer, add it and cover again for 3 days.  When you see a little mycelium poking out of the casing, that's when you start fruiting.  

I didnt plan to use a casing, I was just gonna let it do its thing in the bag out of fear of getting trich again. i'll make sure to do the towel. 

 

Ill post again in 6 days! thanks for all your help guys.


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#20 roc

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Posted 08 June 2019 - 07:30 PM

Couple of comments...

 

On the ratio of spawn and substrate I'd do a 1 to 2 so you'll get a fast colonization to beat any contamination.

I use a 1 to 4 ratio these days but I'm using straight pasteurized horse shit and contamination is rare. This is one of the reasons I love horse shit!

 

It's probably safer for you to either case with straight medium size vermiculite or don't case at all.

 

Great advice from Crazy and Lego!!!


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