I am just replying to thank users Myc and KapnDank! I will get some images soon. I have bought supplies already to try PF since I seem to be having so much difficulty so far and from what you have told me, I will be better off trying PF with agar being the best option. And as far as spores are concered, I do not have access to any from the wild to make spore prints from so I am stuck trusting suppliers until I get a successful grow to make my own. I have researched grain bag and PF and agar and have no issues trying agar but I am not sure I have a clean enough environment for agar until I can get a laminar flow HEPA. So, I will assemble my PF jars and give that a try. I still have two syringes of Golden Teacher which I will try with the PF. I have more syringes of another strain "
Alacabenzi" on the way and I ordered them double strength. I am just trying to get something going so I can make my own spores. The syringes are expensive!
I am starting off by using the smaller 8oz straight sided mason jars for PF. I know that for grain bags I should usie 1ml liquid spores per pound grain, but for PF with a smaller spawn medium of 8oz, I am unsure how much spore liquid to inject to keep moisture levels correct. I plan on using the standard brown rice flower and I have self healing injector ports and .45um PTFE heat resistant filter discs and food grade high temp silicone RTV to glue them onto the lids. I should be able to reuse the lids since everything is high temp and inorganic. I have a small machine shop so I will use a diamond hollow core drill bit to make the holes in the lids which will be almost laser perfect without deforming the lids. I am using Robert McPherson's recipe.
So, any recommondations as to how much fluid to use in a smaller 8oz (.5 pint) jars?
Sounds like you have a solid plan. I loved working with alacabenzi. They gave me nice fat fruits with caps larger than my palms.
A flowhood is like a Cadillac. It's not necessary but man does it make thing go so smoothly.(I love my flowhood, best investment in a long time) you can also do agar in a SAB (still air box) although it'll take some practice learning to use one. They're basically just a large clear tote flipped upside down on a clean surface with two arm holes in the side of the tote.
Start a thread in the cultivation area and I and many others will be happy to help you along on your grow. I'll personally keep an eye out for it. Welcome and mush love
Edit: oh yeah 1ml per jar for pf cakes. It's best to make 4 innoculation holes, evenly spaced, and close to the edges. Inject 1/4ml in each hole. Also high temp rtv will work perfectly fine but regular clear silicone works(adheres) a little better and it's still tough enough to withstand sterilization temps
Edited by KapnDank, 12 August 2019 - 03:37 PM.