IME you have to sterilize grain bags longer than jars because it takes longer to get heat into the center of the grains. Also if your innoculating grains directly with spores there can be mixed results.
I am a complete myco noob and have been searching the threads on this site for a few weeks now and thought I would join to get more detailed info about my particular situation. I also know that it is a real pain when someone asks questions that are easily located in other threads. I will continue to search for a while longer and then post questions after I finish pulling my hair out of my skull! I have started my very first grain spawn over the past few months with nothing but failures so far. My problems are not contamination related. More like mycelium growth problems. My growth always stalls. I will get a golf ball sized growth and then nothing, thats it. I can get some more growth by breaking the ball up (I am using bags) but it stalls shortly after. At first I was thinking low moisture content but I can see light condensation in the bag. All I can think of with my very limited experience is that my supplier might be sending me really old stale spores. I would love to get input. I know I just said that I would look for answers a bit longer before posting but I figured I would ask anyway while I continue to search for this particular problem. Maybe someone has a link?
Spore syringes will have some contams in them, usually bacteria which makes going spores to grain pretty iffy. You can try going spores>agar>grain. That'll ensure you have a clean culture to work with(which I recommend) or, if you don't feel like tackling agar just yet, you can try making PF cakes and crumbling them into bulk as your spawn. Cakes are more contam resistant than grains. Everything loves sterile grains lol
Edited by KapnDank, 11 August 2019 - 08:20 PM.