Now that I started the hobby again, I decided to skip the PF tek jars and go straight into agar.
Also decided to grow sclerotia producers because they can be set and forget.
Here are my notes on agar, please correct the things that I may have wrong:
3gr BRF or potato flakes or DME
optional food color
- more agar (up to 6gr ) makes it harder/stiffer which is easier in transfers.
What would the disadvantage be? ( Harder for spores to germinate? Harder for the mycelium to run into it and therefore slower and more aerial growth? ) - for this line I would appreciate a comment.
- more nutrient means that the mycelium does not need to "move" a lot to find food, therefore slower and more tomentose grow.
That means it may nor run away from bacteria if this was the motivation for agar transfers. (in that case go to 1gr/200ml)
More nutrient also promotes the grow of sclerotia (good in case you grow in agar in order to isolate good sclerotia producing characteristics) - Up to 8gr/200ml
I'm now in my 3rd transfer and I'll prepare rye today or tomorrow.