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Did I Doom My Mycelium? (Shaking Inoc. Jars)


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#1 SeArChEr4

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Posted 09 June 2019 - 03:14 PM

As a new member and BRAND new inductee to using/growing psilocybin, I have been drinking from this firehose of information and opinion about TEKS, tool, etc. After a plagued agar attempt, a BRF tek was used, inoculating 5 jars. 2 of the 5 jars successfully grew mycelium and have continued strong these past few weeks. Through all my reading, I kept coming across the idea of shaking your mycelium jars to break-up and this spread the magic more rapidly. Of my jars that were successfully on route to the birthday, one is an inch or so away from fully engulfing the jar; the second however is only about half-way encased (and spreading). So naturally, in my inexperience I grabbed the second jar whose growth is far behind the first, and shook it vigorously to see if I could get some break up... what resulted was that some of my dry verm. top layer fell down the sides of the jar all over the beautifully white growth. Oh man....here’s a couple pics showing a 360 degree picture of the “shaken one” with the flash ON & OFF. I’ve had a lot of bad news with these processes so far community, don’t break my heart again please lol and as always, I appreciate your time and guidance.

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#2 NewMycoDisciple

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Posted 09 June 2019 - 03:34 PM

Let me preface by saying I am an amateur at best in your same shoes starting in a few days. I too have been soaking up information. It's my understanding when you shake them up you distribute the myc but it stunts growth form a short period.

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#3 Myc

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Posted 09 June 2019 - 03:41 PM

The intent of shaking is for when you're using grains as spawn. The idea is to break up colonized areas and spread mycelium around the jar to the individual particles of grain. 

 

PF Tek really shouldn't be shaken. The dry vermiculite layer is there to act as a sort of filter to prevent contamination from invading the jar. If this layer becomes significantly compromised the project may fail. 

Let those jars recover in a nice dark pantry. They will likely be OK. 


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#4 jkdeth

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Posted 09 June 2019 - 06:19 PM

What Myc said. Shaking is for grains, with filtered lids. But the cake isn't doomed. Chances are it can recover fine. It will either recover or contaminate. You should know in a week.

Patience! Developing patience is important.
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#5 joeya

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Posted 09 June 2019 - 08:37 PM

As myc and jk both said, shaking is for grains, but you're probably ok. The one thing I notice, and which may make a difference, is a lack of micropore tape. The standard PF tek calls for placing micropore tape over the holes after you inoculate the jar. Many people don't bother with this, because the dry vermiculite layer creates a fair barrier against contamination. In your case, that barrier may be compromised, and the micropore tape could be a reasonable insurance policy. So, what's done is done, and you are just going to have to hope for the best outcome (which is still rather likely to attain) but if the holes are not covered with that tape, it is the one thing which you can do at this point to improve your chances. 

Good Luck!



#6 SeArChEr4

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Posted 03 July 2019 - 10:24 PM

First, I’d like to say thank you to all who responded. I have been continuing on my fruitless pursuits into mushrooms since this post but it’s crazy how much one can pick up about this way of life in just some weeks

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#7 SeArChEr4

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Posted 03 July 2019 - 10:38 PM

...My apologies to you all for not responding to your input, I honestly just didn’t check this thread since the post and assumed it stayed quiet until earlier today. So again, thank you and I thought I would include a couple snaps of said earthquake jar to show its progress but also to pose yet another question

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#8 SeArChEr4

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Posted 03 July 2019 - 10:40 PM

... (Wow, this is literally my 3rd time typing this ) The question being, is there a time limit on how long is “acceptable” for milestones like full-colonization, pinning, etc. The only strain I have inoculated spores of is Golden Teacher... is this a notoriously slow- mover? This jar (albeit likely inhibited by my shaking) has only just encompassed the inside of the jar, even with that said... the latest mycelium growth is sparse and less fluffy looking than the earlier/faster myc. Only within the last 2-3 days has the white mycelium encompassed the inside of the jar and even still, the last bit of cake it worked through hasn’t made much progress in even longer. Another point I’d like to mention that might illuminate my misgivings further, is that in the case of a separate jar of GT, today marks the 14th day since it was birthed, rolled, and placed in its FC. To date there is only one area to speak of that shot out any substantial growth but not in a normal healthy fruit. This is more of a cluster of caps that expanded quite rapidly and have been fairly stagnant aside from some bruising as a result of the terrarium roof falling. Aside from this little bulbous growth, there is no signs of new myc. growth, pinning, etc. Like... what the hell, ya know? Lol but anyways this original post was cut off but I appreciate you tuning in and bearing the length and format. I hope more feast falls your harvests than have mine




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