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Misfit’s Horror Hotel - Agar and grow log


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#141 RainbowCatepillar

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Posted 07 September 2019 - 12:13 AM

Wow, what a beautiful specimen! That wavy cap is marveloussss!



#142 Misfit

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Posted 07 September 2019 - 10:27 PM

Current projects are dwindling. Think it’s time for a break to regroup. Got some agar projects in work. Gonna give them some time.
When do you guys give up the ghost on bins? When absolutely no more fruit shows up? It is still too hot here to dump it outdoors with any chance of it surviving.


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#143 KapnDank

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Posted 08 September 2019 - 01:01 AM

Either until there's no more fruits or mold starts to pop up. Whichever happens first.

#144 Misfit

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Posted 08 September 2019 - 07:17 AM

Either until there's no more fruits or mold starts to pop up. Whichever happens first.

I had one or two that only put out 1-2 fruits. Figuring they are pretty much dunzo.


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#145 KapnDank

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Posted 08 September 2019 - 08:21 AM

Yeah probably so. Have you dunked or tried bottom watering? Might get a little more out of them if you haven't but either way it's probably pretty much spent.

#146 KapnDank

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Posted 08 September 2019 - 08:21 AM

Yeah probably so. Have you dunked or tried bottom watering? Might get a little more out of them if you haven't but either way it's probably pretty much spent.

#147 Misfit

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Posted 08 September 2019 - 08:25 AM

Oh yea. I have. This is probably their 4th push. I’m not complaining. They have fruited a ton of beautiful fruits.


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#148 Misfit

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Posted 10 September 2019 - 01:49 PM

5e7b0590dc7c2a22504584dd14fb06e0.jpg
These things just won’t stop fruiting. I really wish they didn’t drop spores so hard.


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#149 KapnDank

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Posted 10 September 2019 - 02:56 PM

What are those? Digging the nippled caps
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#150 Misfit

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Posted 10 September 2019 - 02:57 PM

It’s a Redboy hybrid @coorsmikey was working on. I can send you the thread link if you want.


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#151 Misfit

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Posted 10 September 2019 - 02:59 PM

https://mycotopia.ne...s-for-mycology/


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#152 Misfit

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Posted 30 January 2020 - 05:48 PM

If anyone is interested, I started my first day of internship at my school's mycology lab!! I was also able to enroll in intro to mycology this semester.

Hope everyone is well.


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#153 coorsmikey

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Posted 30 January 2020 - 07:25 PM

If anyone is interested, I started my first day of internship at my school's mycology lab!! I was also able to enroll in intro to mycology this semester.

Hope everyone is well.

 

Ha in a few months I will be expecting to see some new shit to learn. Ya better post!


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#154 Misfit

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Posted 30 January 2020 - 09:18 PM

One thing I learned today is a small amount of lactic acid was added to PDA premix. The higher acidity of the agar wards off unwanted contaminants. The mycelium isn’t affected by it though.
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#155 Asura

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Posted 31 January 2020 - 12:30 AM

How small are we talking?



#156 Misfit

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Posted 31 January 2020 - 06:35 AM

I believe 750ul was added to 500ml. I didn’t catch what the percentage was, but I can get more info next week for sure.
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#157 Misfit

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Posted 06 February 2020 - 06:48 PM

So an update...it was a 10% solution. I gotta snap a picture of the agar recipes. I was working with one that has V8 in it. I got to do a ton of transfers today. Got to see some really beautiful pink Fusarium growth. I really fell short not getting any pictures.If anyone is into tribal, electronic music with a SE Asian feel please so check out Shivelight on YouTube or Spotify. That shit put me in the zone working in the hood.

I got to learn of a storage procedure for Ascomycota spores. They are suspended in skim milk solution. Then stored on silica crystals. This process heats the Silica. Today I placed some silica that was recently prepared on some various agar plates to verify the spores took properly. I can update some pics a week from now.

Side note to anyone still reading. I emailed Mr. Stamets a thank you for the inspiration. He responded within 5-10 minutes. I emailed the company info email address for info on internships or summer research opportunity..IM DOING THIS MY DUDES...and dudettes!!

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#158 joeya

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Posted 06 February 2020 - 07:52 PM

Glad to see you moving in this direction! I have a feeling you're going to be posting a lot of innovative stuff!


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#159 Misfit

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Posted 06 February 2020 - 08:44 PM

Glad to see you moving in this direction! I have a feeling you're going to be posting a lot of innovative stuff!

My brother, how have you been? Thank you for the support!

Oh side note, I think I can propose my own research projects starting next year.


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#160 joeya

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Posted 06 February 2020 - 10:31 PM

I'm good. Have a tray of acadia coasts in the fruiting chamber, just a few pins on them right now, but looks promising! Got several jars of spawn ready to go behind them soon, they are certainly an aggressive colonizer!


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