Paradox
©
Fisana

Jump to content


Photo
* * * * * 4 votes

How I Grow Cubes: Featuring Ps. cubensis "Martinique"


  • Please log in to reply
28 replies to this topic

#1 DonShadow

DonShadow

    Mycotopiate

  • OG VIP
  • 622 posts

Awards Bar:

Posted 01 July 2019 - 02:41 PM

Here's a quick overview of how I grow Cubensis. There isn't really anything new here, just a streamlined dub-tub TEK that I feel is very simple/beginner friendly. The strain featured was harvested from the Caribbean island of Martinique. The print was gifted by a kind member here, the only 'Topian that I've had the honor to meet in the flesh. It seems to be still fairly wild, and produces very dense, potent fruits. Although I was working with agar, this TEK requires no flow-hood or glove-box, and typical incubation temperatures (77-82F-ish) are not required when clean grain-spawn is used. My dub-tub was spawned in open air, and both grain spawn and tub were incubated at room temperature to show that sterile conditions and incubation temperatures are not necessary for a good flush of cubensis. As long as your grain spawn is clean, you'll be fine.

 

 

After an initial multi-spore test grow, a tissue sample was selected from an ideal specimen, and a rhizomorphic sector was isolated in two transfers.

 

A jar of whole oats was then prepared using the following method:

 

Makes one grain spawn jar per 300ml (1/3 quart jar) of grain.

Works for rye berries, wheat berries, whole oats, millet, wild bird seed, and probably many others.

 

Ingredients

  • 300ml grain per desired jar of grain spawn

  • 1 pinch of gypsum per jar *optional* (prevents clumping)

  • Three times the volume of water as grain (eg. 600ml grain = 1800ml water)

 

Procedure

- Before you begin, calculate how many jars of grain spawn you will need (1 jar will inoculate 1 dub-tub). 1/3 quart of dry kernels will result in 1/2 to 2/3 quart of hydrated kernels. A grain spawn jar should never be filled more than 2/3, or it will colonize slowly and will be very difficult to break up once colonized.

- In an appropriately sized pot, boil reverse osmosis water, around three times the volume of your dry grain.

- While the water is heating, rinse your grain well over the sink with lukewarm water in a fine mesh strainer or colander (tap water is fine for this step).

- Once the water has boiled, dump your rinsed and drained grain into the pot.

- Bring to a boil again just briefly if necessary, then immediately remove the pot from the hot element. Cover with well-fitting lid.

- Set a timer for 45 minutes.

- After 45 minutes has passed, remove the lid to inspect the grains. If they are well hydrated, 10-15% of the kernels will have burst open. If you don't see any burst grains, replace the lid and check back in 15 minutes. It usually takes between 45-70 minutes for proper hydration using this method.

- When it appears that the grains are 10-15% burst, pour into mesh strainer and allow to drain for one hour, mixing the grains well three or four times over the course of the hour.

- After the hour has passed, mix well and divide equally into your regular-mouth jars. Fill each jar no more than 2/3-full.

- Fit jars with grain spawn lids (micron filters and injection ports). Lids should be snug, no need to overtighten. Cover all lids with tin foil.

- Place in pressure cooker and sterilize for 90 minutes at 15PSI.

- If your grains looked a little over-hydrated before sterilizing, you may wish to remove them from the cooker as soon as the pressure drops so they don't overcook. If they looked a little under-hydrated, consider leaving them in the cooker until it cools to room temperature, so that the kernels get a little softer.

- After removing from the cooker, check lids for tightness (snug is ok, DO NOT overtighten or open the lids).

- Before inoculating, shake/bump the jar gently to distribute moisture and break up clumps.

- Proceed to inoculate with the mushroom species of your choice (agar, liquid culture and multi-spore solution are all fine).

 

________________________________________________________________________________________________

 

 

For my grow the jar of whole oats was then inoculated with a few agar wedges from my cloned culture, and I also inoculated a liquid culture for later use.

 

Jar was shaken vigorously for one solid minute, and left to incubate at ~72F for 14 days.

 

HOWEVER, this TEK assumes you are not working with a glove box or a flow hood, so you will be using either an LC or MS syringe to inoculate your grain using the following technique:

 

Inoculating Grain Spawn

 

- Work indoors and close windows, doors etc. to minimize airflow and airborne contaminants. Wearing a surgical mask is a good idea.

- Disinfect hands and arms well with lots of alcohol (repeat throughout process as necessary).

- Disinfect work area by wiping surfaces with 70% isopropyl alcohol.

- Disinfect the entire surface of your grain spawn jar, taking extra care to wipe the injection port.

- Disinfect your LC syringe or multispore syringe, and a new needle if you will be using one.

 

If working with a new needle:

- Quickly remove the syringe cap or old needle and replace with a new sterile needle tip.

 

If working with a used needle:

- Wipe syringe and needle with alcohol. Wait for alcohol to evaporate completely, then flame the needle tip until it glows red.

- Wait 10-15 seconds for needle to cool, or dip the tip in a small dish of alcohol.

 

Either or ^^

- Wipe the injection port on your grain spawn with alcohol and fully insert your needle.

- Aiming the needle at the sides of the jar, shoot about 0.5ml to 1ml/cc of liquid at each of the four sides of the jar.

- After removing the needle, always wipe the injection port to remove any residue that might attract contaminants.

- Quickly re-cap your needle or remove the needle and replace with a disinfected luer-lock syringe cap.

- Store syringe in zip-loc bag in the refrigerator.

 

Incubating Grain Spawn

 

- Incubate your grain spawn at 77-82F if possible, but this is not necessary for this TEK.

- If inoculated with LC or an MS syringe, wait until you see streaks of mycelium covering about 15-20% of the visible grains next to the glass. This should take about 4-6 days. Now shake the jar very well to evenly distribute the inoculated grains throughout the jar.

- Wait until the entire jar is densely colonized and white with mycelium. This will likely take between 10-16 days.

- Once fully colonized, you may use the jar to inoculate a bulk substrate or store in the refrigerator for up to 1 year.

___________________________________________________________________________________________

 

 

Bulk substrate was prepared using the following recipe:

 

Supplemented Coir Substrate

Makes enough substrate for two dub-tubs (about 4 quart jars packed lightly).

If you only need enough substrate for one dub-tub, simply divide recipe in half.

 

Ingredients

  • 275g dry coir

  • 100ml used coffee grounds

  • 100ml grain flour or bran (eg. oat, brown rice, wheat, quinoa, millet. I like oat bran best)

  • 100ml clean dry sand

  • 25ml gypsum

  • 25ml Calcium carbonate (CaCO3)

  • Approximately 1300ml Reverse osmosis water

 

Procedure

- Place coir in large pail and break it apart as well as you can (1-inch chunks or smaller).

- Add gypsum.

- Pour in approximately 1 litre of boiling R/O water to hydrate.

- Mix well and cover with lid for 10 mins.

- After ten minutes has passed, mix again and and break up all chunks of coir by hand.

- Add remaining ingredients and mix well by hand to soften all the lumps.

- Bring to field capacity* and load into wide-mouth jars. Pack jars by tapping the bottom of the jar lightly on a firm surface and fill right to the brim.

- Fit jars with wide-mouth metal lids, but leave the rings loose. DO NOT tighten lids, or your jars may explode. You'll tighten them once they have been sterilized.

- Place in pressure cooker and sterilize 90mins at 15PSI.

- As soon as pressure drops to zero, remove jars and tighten lids to create a vacuum seal. Use oven mitts or leather gloves and handle the jars very carefully. As long as a good seal is achieved, jars will store at room temperature indefinitely, so don't worry about making too much substrate.

 

 

*How to test for field capacity (proper soil hydration)

- Once all ingredients have been combined and mixed well, grab a good handful of soil and squeeze tightly in your fist. If the soil is properly hydrated you should see a small amount of water pooling around your fingers/knuckles when you squeeze, but it should not trickle down your hand. One or two drops is ok.

________________________________________________________________________________________________

 

 

Preparing the Dub-Tub:

 

I buy 4-quart PP5 shoebox tubs from a local dollar store for $2 each. They come with tight-fitting lids, which I drill holes in for gas exchange.

 

I use a step drill-bit to drill four 3/4" holes in the bottom tub, about 3.5" in from the sides and as close to the rim as I can get:

 

Photo 2019-07-01, 10 30 31 AM.jpg

Photo 2019-07-01, 10 31 07 AM.jpg

 

I use the same step drill-bit to drill four holes on the top tub, about 3.5" in from the sides, just on the middle of the curve:

 

Photo 2019-07-01, 10 31 41 AM.jpg

 

You can stack the tubs together and mark them to make sure your holes are aligned before you drill:

 

Photo 2019-07-01, 10 31 57 AM.jpg

 

Clean off the plastic residue and burrs with a razorblade.

 

Drill a few holes in the lid using a 1/4" pilot-point drill-bit:

 

Photo 2019-07-01, 10 34 43 AM.jpg

Photo 2019-07-01, 10 36 08 AM.jpg

____________________________________________________________________________________________

 

 

Spawning to the Dub-Tub

 

- Work indoors and close windows, doors etc. to minimize airflow and airborne contaminants. Wearing a surgical mask is a good idea.

- Disinfect hands and arms well with lots of alcohol (repeat throughout process as necessary).

- Disinfect work area by wiping surfaces with 70% isopropyl alcohol.

- Break up grain spawn by knocking the bottom of the jar repeatedly on a padded surface such a the arm of a couch or a bicycle tire. Don't strike the jar in the middle where it is weaker. Shake well to break up all chunks. This may take some oompf.

- Disinfect your bottom tub and blue tub lid, your grain spawn jar, your substrate jars and a stainless steel spoon.

- Cover the holes in the tub lid with micropore medical tape (the breathable 3M cloth tape, not the plastic stuff).

 

Photo 2019-07-01, 10 35 54 AM.jpg

 

- Cut a plastic garbage bag so that the sterile inside of the bag will be facing up when you separate the layers and line your tub. If you're re-using a bag that you cut for a previous tub, wipe down both sides with alcohol. 1 average sized garbage bag is usually good for 4 tubs total.

- Once the plastic liner has been fitted well in the tub and the unused layer has been removed, work very quickly and dump in two jars of sterilized substrate and one jar of broken up grain spawn.

- Mix everything very well with your disinfected spoon, taking care to evenly distribute all the kernels of grain spawn very well throughout the tub. Pay close attention to the bottom and sides to make sure there aren't any large patches of substrate without any grain spawn. Try not to tear the liner.

-Once the spawn is thoroughly mixed, level out the surface, but leave shallow ridges and grooves on the top. You want it level, but not flat. The ridges create micro-climates and encourage more mushrooms to grow.

 

Photo 2019-05-16, 10 31 32 AM.jpg

 

- When you're satisfied, make sure the liner is well-formed to the sides of the tub, with the excess hanging over the edge. Now place the lid on top; the liner should be sandwiched between the rim of the tub and the lid.

- Once the lid has been locked on, gently run a razor blade along the rim to cut off the excess plastic where it sticks out.

 

Photo 2019-05-16, 11 19 13 AM.jpg

 

- Label and date your project.

- Incubate between 77-82F if possible, but this is not necessary for this TEK. Keep out of direct sunlight (ambient light is fine). The cooler the incubation temperature, the longer it will take to colonize. If you don't have an incubator, you may use a closet with a space heater (just monitor the temperature well) or place it in the warmest location in the house.

- When fully colonized, the entire surface of the tub should appear white and fluffy. If your tub has a plastic lid you can use a flashlight to look through the plastic lid and check the progress. Be patient and don't mess with it too much. Condensation will build up on the underside of the lid; don't worry about it.

 

My tub fully colonized in 16 days, incubated at room temperature (~72F).

__________________________________________________________________________________________________

 

 

Peat Moss Casing

Makes two quart jars 3/4 full

 

Ingredients

  • By volume: 10 : 1 : 0.5 = Peat moss : Calcium carbonate (CaCO3) : Sand

  • 1500ml finely crumbled peat moss

  • 150ml CaCO3

  • 75ml sand (well-rinsed fine beach sand is good)

 

Procedure

- In a large pail, mix all ingredients well.

-Add reverse osmosis water in small increments until field capacity is achieved.

- Load equally into two wide-mouth jars. They should only be 3/4 full so that you can shake them to distribute moisture equally before use.

- Fit jars with wide-mouth metal lids, but leave the rings loose. DO NOT tighten lids, or your jars may explode. You'll tighten them once they have been sterilized.

- Place in pressure cooker and sterilize 90mins at 15PSI.

- As soon as pressure drops to zero, remove jars and tighten lids to create a vacuum seal. Use oven mitts or leather gloves and handle the jars very carefully. As long as a good seal is achieved, jars will store at room temperature indefinitely, so don't worry about making too much casing.

____________________________________________________________________________________________

 

 

Applying Casing Soil and Closing the Dub-Tub

 

- Once your tub has fully colonized, it's time to apply casing soil and install the top-tub.

- Work indoors and close windows, doors etc. to minimize airflow and airborne contaminants. Wearing a surgical mask is a good idea.

- Disinfect hands and arms well with lots of alcohol (repeat throughout process as necessary).

- Disinfect work area by wiping surfaces with 70% isopropyl alcohol.

- Disinfect your casing jar and a stainless steel fork.

- Shake the casing jar well to evenly distribute moisture.

- Remove the tub lid carefully. Try not to allow too many droplets of condensed water to fall onto the surface of the substrate.

 

Photo 2019-06-07, 3 20 50 PM.jpg

 

- Apply the casing by allowing it to gently fall off the fork. The final depth should be between 1/4 and 1/2 inch. Make sure to get casing in all the crevices on the sides and cover every bit of white. Do not apply pressure or compress the casing, you want it to be as fluffy and airy as possible.

- Once the casing has been applied, use a razorblade to carefully cut out the liner where your holes on the bottom tub are located.

- Using a ball of polyfil about the size of a small egg, stuff the four bottom holes very loosely, pushing through from the outside and pulling in from the inside. Don't worry if the polyfil touches the casing.

 

Photo 2019-06-07, 3 38 07 PM.jpg

Photo 2019-06-07, 3 37 42 PM.jpg

 

- Disinfect your top tub and stuff the holes in the same way that you did with the bottom tub.

 

Photo 2019-06-07, 3 38 31 PM.jpg

 

- Place the top tub edge down on the bottom tub to form a little dome.

 

Photo 2019-06-07, 3 39 26 PM.jpg

 

- At this point you may sandwich the excess liner between the tub rims and cut off the excess, or simply leave it inside the tub.

 

Photo 2019-06-07, 3 39 35 PM.jpg

Photo 2019-06-07, 3 41 35 PM.jpg

 

- Using scotch tape, seal the two tubs together by taping all around the rim. This doesn't have to be pretty.

 

Photo 2019-06-07, 3 49 35 PM.jpg

 

At this stage there is no need to open the tub again until harvest time.

____________________________________________________________________________________________

 

 

Fruiting Your Dub-Tub

 

- Place the sealed tub somewhere out of reach of animals and children. A shelf or mantle is good, but not a windowsill.

- The tub should be exposed to ambient daylight, but no direct sunlight.

- Room temperature is fine, 70-85F is ideal. Lower temperatures will produce more potent mushrooms, but they will grow more slowly.

- It is very beneficial to have a fan blowing on a low setting 24hr/day in the fruiting room. Just don't point the fan directly at the tub.

- White blobs of mycelium appearing on the surface of the casing is not a sign of contamination, but normal healthy growth. The mycelium may almost completely colonize the casing, this is normal and nothing to be worried about.

- If you see a lot of moisture collecting on the tob of the dub-tub or substrate surface, you should loosen your polyfil to allow for more airflow and evaporation. This is the only maintenance you should need to do, otherwise this TEK is "set it and forget it".

- You can expect to see primordia (pinheads) in about 8-14 days. The fruitbodies will likely fully mature between 5-10 days after primordia formation. The mushrooms are mature as soon as the veils break and expose the gills.

- You may see white fluff climbing up the stipes of your mushrooms. This is just mycelium, totally fine.

- As soon as the veils break, the mushrooms will begin dropping spores. If you see purple-brown gunk everywhere, don't be alarmed; it's the miracle of life. Try to pick them before they sporulate because it can get messy fast, and spores taste terrible.

- Your mushrooms may grow so large that they push up against the top of the dome. Don't worry about it, just let them grow.

_____________________________________________________________________________________________

 

 

Harvesting and Cleaning Your Mushrooms

 

- As soon as the caps of the mushrooms begin to open and the veils fall away, it's time to harvest.

 

Photo 2019-06-30, 11 36 41 PM.jpg

 

- Peel the tape away from the rim of your tub and remove the top.

 

Photo 2019-06-30, 11 37 01 PM.jpg

 

- Wash your hands.

- Harvest the mushrooms by cutting them with scissors as close to the base as you can, avoiding disturbing the casing too much. Some people prefer to twist/pull to harvest, and that's totally fine. I like to cut them because it leaves less mess to clean on both the substrate and the mushrooms.

- Clean the mushrooms well with a soft-bristle brush, and use your scissors to cut off any casing stuck to the base of the stipes.

- Undeveloped mushrooms may be allowed to continue growing after harvesting the mature ones. Simply replace the top tub (no need to replace the tape) and harvest again when they are mature.

- Some mushrooms will stay tiny and never mature. These are called “aborts”. You may pick these; they're fine to eat and are quite potent. An abort will often have a blue or black cap and start growing fuzzy mycelium. If it shows no growth in a day or two, it's definitely an abort. Pick it, toss it, or if it's really tiny, just leave it alone; it will be reclaimed by mycelium.

_________________________________________________________________________________________________

 

 

Rehydrating Your Tub

 

There are two methods. Method 1 takes more effort but results in better fruiting. Method 2 is much easier, but will yield fewer fruits.

 

Method 1

- Fill a jar or jug full of tap water. Seal the lid tightly and clean the outside of the jug.

- Place jar on its side on top of the substrate and place the tub on a cookie sheet to prevent spillage.

 

Photo 2019-07-01, 1 27 02 AM.jpg

 

- Using reverse osmosis water, completely fill the margin between the tub wall and the liner, then fill the surface of the substrate. The water on the outside prevents the liner from separating from the tub, and the milk jug stops the substrate from floating.

 

Photo 2019-07-01, 1 30 19 AM.jpg

 

- Cover with the top tub and leave to absorb water for 12-24 hours.

- After at least 12 hours has passed, place your hand on the jug to hold it in place and pour out all the water from the substrate and the tub. Remove the jug and use a clean hand directly on the substrate surface to hold it in place while you allow residual water to drip out.

- Apply a second light casing layer (1/4 only) and replace the top tub/tape. Resume fruiting conditions.

 

Method 2 (the lazy method)

- Spray the shit out of your substrate with reverse osmosis water, just until water begins to pool.

- Replace the top tub, and you're done. Return to fruiting conditions.

 

 

Preserving Your Mushrooms

 

- In my opinion, the best way to preserve mushrooms is to fully dehydrate them and store them in a well-sealed glass jar with a desiccant pack. Take my advice. You've already put in so much work, so why fuck it up now? Use a dehydrator. Just do it, there is no easier/more through way to dehydrate mushrooms. You can find a perfectly good one for under $40.00. If you need to borrow one, do that.

- Your mushrooms are fully dehydrated when they don't bend at all when you apply force. This is called “cracker dry”. You should be able to easily crumble them to dust with your fingers. This may take up to two full days for larger specimens. My dehydrator has a temperature control, I use 50C, but a little warmer or cooler is fine.

- Slice large mushrooms in half to dry them more quickly.

- Once you're sure your mushrooms are “cracker dry”, place them in a clean, well-sealing glass jar with a fresh desiccant pack. It's a good idea to weigh them first, and mark the weight on the jar with a label or a piece of masking tape. When you add more or take some out, simply add or subtract the weight so you always know how much is in the jar.

- You can purchase desiccant packs online in bulk or make them yourself by purchasing unscented silica cat litter and placing a small quantity of litter in a paper or Tyvek packet tied with string or taped up.

 

Photo 2017-11-16, 7 31 25 PM.jpg

Photo 2017-11-16, 7 37 16 PM.jpg

Photo 2017-11-16, 7 39 29 PM.jpg

Photo 2017-11-16, 7 40 31 PM.jpg

 

- Store the jar at room temperature, or in the freezer. Just be aware that if you open an ice-cold jar in a room temperature environment, it will instantly create condensation INSIDE of the jar. So, if you store your jar in the freezer, always allow it to come to room temperature before opening it.

- PRO TIP: Have a large “master jar” and a smaller “frequent use” jar. The larger master jar will be opened infrequently and therefore will preserve longer. The frequent use jar is smaller and will be used up too quickly to degrade.

- PROPERLY PRESERVED MUSHROOMS WILL LAST FOR YEARS! CRACKER DRY + SEALED GLASS JAR + DESICCANT = ❤️

 

Photo 2019-06-30, 11 37 12 PM.jpg

Photo 2019-06-30, 11 37 30 PM.jpg

Photo 2019-06-30, 11 37 50 PM.jpg

Photo 2019-06-30, 11 38 04 PM.jpg

Photo 2019-06-30, 11 38 14 PM.jpg

Photo 2019-06-30, 11 38 24 PM.jpg

Photo 2019-06-30, 11 38 36 PM.jpg

Photo 2019-06-30, 11 38 48 PM.jpg

Photo 2019-06-30, 11 39 01 PM.jpg

Photo 2019-06-30, 11 39 26 PM.jpg

Photo 2019-06-30, 11 39 36 PM.jpg

Photo 2019-06-30, 11 51 29 PM.jpg


  • onediadem, coorsmikey, peacefrog and 11 others like this

#2 peacefrog

peacefrog

    Peaceful Frog

  • Moderator
  • 2,368 posts

Awards Bar:

Posted 01 July 2019 - 02:56 PM

Beautifully done my friend!

Great log for sure. This will help a lot of folks. Thank you taking the time to put this together and share it with all of us.

One for the archives.

Good vibes.
  • CatsAndBats and DonShadow like this

#3 CatsAndBats

CatsAndBats

    This motherfucker

  • OG VIP
  • 12,207 posts

Awards Bar:

Posted 01 July 2019 - 04:44 PM

I love your detailed process and writing. Thanks for sharing this.


Edited by CatsAndBats, 01 July 2019 - 04:44 PM.

  • coorsmikey and DonShadow like this

#4 onediadem

onediadem

    Insidious Drivel

  • OG VIP
  • 16,397 posts

Awards Bar:

Posted 01 July 2019 - 05:44 PM

Nice write up. I have never understood pre boiling any grains beside popcorn. 1c grain to 2/3c water in any combination always makes perfect spawn for me. Load and go.


  • coorsmikey, bezevo and DonShadow like this

#5 joeya

joeya

    Mycotopiate

  • Black VIP
  • 483 posts

Posted 01 July 2019 - 05:57 PM

This is pretty much foolproof, great write-up!


  • DonShadow likes this

#6 DonShadow

DonShadow

    Mycotopiate

  • OG VIP
  • 622 posts

Awards Bar:

Posted 01 July 2019 - 06:03 PM

I'm glad to share, I've learned so much from everyone here, so sharing a few teks is the least I can do.

Thanks for that suggestion OD, I've never used that method for any grain aside from grass seed. Do you rinse the grain first? And I'm guessing you mean 1 cup grain to 2/3 cup water? That'll shave a lot of time.
  • roc likes this

#7 CatsAndBats

CatsAndBats

    This motherfucker

  • OG VIP
  • 12,207 posts

Awards Bar:

Posted 01 July 2019 - 06:14 PM

Nice write up. I have never understood pre boiling any grains beside popcorn. 1c grain to 2/3c water in any combination always makes perfect spawn for me. Load and go.

 

 

I'm glad to share, I've learned so much from everyone here, so sharing a few teks is the least I can do.

Thanks for that suggestion OD, I've never used that method for any grain aside from grass seed. Do you rinse the grain first? And I'm guessing you mean 1 cup grain to 2/3 cup water? That'll shave a lot of time.

 

 

Gotta shake them as soon as they're cool enough to handle though, or you're going to spend a lot of time digging them out, at least with oats IME.


  • roc and DonShadow like this

#8 onediadem

onediadem

    Insidious Drivel

  • OG VIP
  • 16,397 posts

Awards Bar:

Posted 01 July 2019 - 10:13 PM

Yes, you have to shake the jars hot. That is what oven mitts are for. I use bags most of the time and let them sit in the pc until I need to use them. Much easier to break up. I do start my masters in jars though. I have just never understood why you would pre cook when you dont have to.


  • Microbe and DonShadow like this

#9 onediadem

onediadem

    Insidious Drivel

  • OG VIP
  • 16,397 posts

Awards Bar:

Posted 01 July 2019 - 10:17 PM

I'm glad to share, I've learned so much from everyone here, so sharing a few teks is the least I can do.

Thanks for that suggestion OD, I've never used that method for any grain aside from grass seed. Do you rinse the grain first? And I'm guessing you mean 1 cup grain to 2/3 cup water? That'll shave a lot of time.

 

 

 

No rinsing. Yes to the amounts.


  • DonShadow likes this

#10 jkdeth

jkdeth

    Mycotopiate

  • Free Member
  • 2,567 posts

Awards Bar:

Posted 01 July 2019 - 11:14 PM

I'm obsessive about rinsing. I just can't "not rinse". I rinse oats several times with hot water, then dump the grain into boiling water and turn it off, pop a lid on and give it a random amount of time, depending on what else I'm doing. Its usually ready in 15 minutes or so, but may be because of my obsessive rinsing. (I've actually fully hydrated Bird Seed with just rinsing)

I've tried no prep. Worked. Looked horrible. Didn't care for it.

It does take me long time to prep grain though. Its usually a two day affair that nets me 21 quarts. 14 ready and 7 in pc. Sometimes I run pints, but the count is the same.

I also tend to develop odd ritualistic behavior. I have one pot I do grain in. If it disappeared, I don't think I could run grain again until I replaced it with an exact replica. Lol. And 4 to 5 cups dry grain is the max it will hold.
  • onediadem, roc and DonShadow like this

#11 Thacan

Thacan

    Mycotopiate

  • VIP
  • 242 posts

Posted 01 July 2019 - 11:24 PM

Very well done.  Thank you!


  • DonShadow likes this

#12 Misfit

Misfit

    Mycotopiate

  • VIP
  • 1,066 posts

Posted 02 July 2019 - 12:40 AM

I love your write-ups. Thanks for passing on some of the knowledge.
  • DonShadow likes this

#13 Oneyedraven

Oneyedraven

    Mycotopiate

  • Gold VIP
  • 269 posts

Posted 02 July 2019 - 03:22 PM

DS- thank you for this write up..i have been looking for a start to finish Tek to test the bulk tub waters (instead of pf cakes) this post is perfect.  I have bits and pieces of the process but noting that wraps it all up in such a concise and complete way.. kudos 


  • DonShadow and Moonless like this

#14 roc

roc

    Forum International Moderator

  • Moderator
  • 8,085 posts

Awards Bar:

Posted 02 July 2019 - 08:27 PM

Nice write up indeed.


  • DonShadow likes this

#15 Moonless

Moonless

    Mycotopiate

  • Black VIP
  • 701 posts

Awards Bar:

Posted 02 July 2019 - 09:17 PM

Hey DonShadow, Thank you so much for making this one. Myself and lot of members are growing cubes, for alot of us we are new to mycology. I have saved this one in my resources, I liked how you went over how to use an old needle and covered the whole process. Very helpful for any of us who are just starting out.

Cheers!


  • DonShadow likes this

#16 onediadem

onediadem

    Insidious Drivel

  • OG VIP
  • 16,397 posts

Awards Bar:

Posted 02 July 2019 - 11:15 PM

I'm obsessive about rinsing. I just can't "not rinse". I rinse oats several times with hot water, then dump the grain into boiling water and turn it off, pop a lid on and give it a random amount of time, depending on what else I'm doing. Its usually ready in 15 minutes or so, but may be because of my obsessive rinsing. (I've actually fully hydrated Bird Seed with just rinsing)

I've tried no prep. Worked. Looked horrible. Didn't care for it.

It does take me long time to prep grain though. Its usually a two day affair that nets me 21 quarts. 14 ready and 7 in pc. Sometimes I run pints, but the count is the same.

I also tend to develop odd ritualistic behavior. I have one pot I do grain in. If it disappeared, I don't think I could run grain again until I replaced it with an exact replica. Lol. And 4 to 5 cups dry grain is the max it will hold.

 

I am ocd in other areas of life. I keep things as streamlined as I can with this hobby or I wouldn't be able to stand it. I would probably freak a few out with as much as I throw away when cleaning them. We all have our little quirks lol.



#17 jkdeth

jkdeth

    Mycotopiate

  • Free Member
  • 2,567 posts

Awards Bar:

Posted 02 July 2019 - 11:48 PM

Funny thing. Its mostly the grain prep for me that gets obsessive. I get sloppy or adventurous on everything else. I'm all for lazy man way in everything else.

#18 DonShadow

DonShadow

    Mycotopiate

  • OG VIP
  • 622 posts

Awards Bar:

Posted 03 July 2019 - 01:45 AM

Hey DonShadow, Thank you so much for making this one. Myself and lot of members are growing cubes, for alot of us we are new to mycology. I have saved this one in my resources, I liked how you went over how to use an old needle and covered the whole process. Very helpful for any of us who are just starting out.

Cheers!

I'm glad you found it helpful Moonless. I should clarify that what I meant by a "used needle" is one that has already been used to inoculate a jar once before, but not removed from the syringe after use. Spore syringes usually come with a needle, and are probably often stored with the capped needle after using it. In that situation, it's important to flame the needle because it was exposed to potential contamination. I personally wouldn't recommend using a needle like this, and would opt instead to cap the syringe with a sterile luer lock syringe cap, and sterilize the needle before using it again. I save my used needles and reuse them over and over. To sterilize them, I put them in a small mason jar with a grain spawn lid, put about a tablespoon of water in the bottom of the jar and PC for 20 mins. I do all of my work in front of the flow hood, so I just open up the jar in front of the hood and take out as many as I need, but if you wrap them in tin foil before you sterilize them, you could open the jar in plain air without worrying about contamination.


Edited by DonShadow, 03 July 2019 - 01:46 AM.

  • Moonless likes this

#19 SacredMountain

SacredMountain

    Mycophage

  • VIP
  • 119 posts

Posted 04 September 2019 - 11:03 PM

In the section of rehydrating the sub you wrote:

"Apply a second light casing layer (1/4 only) and replace the top tub/tape. Resume fruiting conditions."

 

can you tell me (1/4 only) of what amount?



#20 Misfit

Misfit

    Mycotopiate

  • VIP
  • 1,066 posts

Posted 04 September 2019 - 11:08 PM

Inch


“I’d rather have nothing than have a lie”




Like Mycotopia? Become a member today!