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First ever grow BRF Cakes 6-14


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#1 MushLearner

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Posted 01 July 2019 - 10:31 PM

First pics are of GT I inoculated on the 14th, taken on the 19th.

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Then the same jars pictures taken on the 22nd. Temps were high 60s low 70s this first week


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#2 MushLearner

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Posted 01 July 2019 - 10:36 PM

Then the same jars on the 1st of July! (Today)
This is all of them, of the 12 I inoculated 11 took. So far they seem to be free of contams.

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Some clearly arent going as fast as others..
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#3 MushLearner

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Posted 01 July 2019 - 10:41 PM

These 6 are part of the same experiment, but are b+ spores from the same supplier. These jars were inoculated on the 15th. I hadn't taken any pictures until today so well start further along with them

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So when I made these I sterilized 7 jars and only inoculated 6, leaving one as a control. That jar is clean and everything looks good to me!

Any advice i can get would be good!

My only concern is minor condensation on the walls of the jars I just noticed today. Is that ok?

Edited by MushLearner, 01 July 2019 - 11:52 PM.


#4 Misfit

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Posted 02 July 2019 - 02:12 AM

I’d suggest filling your jars next time. Every time you handle those the dry vermiculite barrier is moved. It pretty much nullifies the safety zone.
Oh and don’t worry much about the condensation.

Edited by Misfit, 02 July 2019 - 02:13 AM.


#5 MushLearner

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Posted 02 July 2019 - 06:58 AM

Yeah, the first batch of 12 have no verm layer left, I wish I had pictures before and after PC because it disappeared on day 1.. the second batch I made (the 6 jars on bottom) did better. Thanks for the tips!

#6 Misfit

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Posted 02 July 2019 - 09:24 AM

It helps to tap the jar on a towel just enough to settle the substrate. Then clean the lip of the jar. I try to load just enough vermiculite in to make a small mound of top. That way when you put the lid on it compresses just a tiny bit.
That non nutritional layer is very important to prevent contams from taking hold.
Best of luck and good vibes.
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#7 Theinvitrowalrus

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Posted 02 July 2019 - 09:30 AM

Looks like good stuff!
Im going to start to first ones this week!

#8 bezevo

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Posted 02 July 2019 - 09:50 AM

WELCOME !

 

GOOD LUCK !



#9 mad420pumpkinz

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Posted 02 July 2019 - 11:53 PM

Looking good nice start :)

#10 MushLearner

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Posted 11 July 2019 - 12:06 AM

So my original plan was just to finish all the cakes as cakes in a monotub style tub.. that plan has now changed. Since I had a much higher success rate than I expected, I've decided to play with some of these jars a little. So I am using a few jars as spawn to a bulk sub, just to see how it goes.

These first pictures are two GT brf cakes, fully colonized but no pins in the jars. Broke them up in a bag and mixed them with 100 percent coco coir. Put the mixture into a small tub with a lid with holes. Hopefully everything will go well! These were done yesterday, the 9th.

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So the next pictures are of the b+ cakes I did today, and I actually had everything I wanted to have today so this bulk is a 50/50 mix of local hpoo and coir, with enough gypsum to white out the water I used to hydrate. This time instead of mashing the cakes in a baggie I used a big cheese grater I have. Forgot to take pictures of them after grating but let me tell you it made for a much finer consistancy

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So just to add to my log here, I started a petri of agar as well. It's all really part of my initial experimentations.. So I knocked the dish on the 3rd with a very very small squirt from a Treasure Coast syringe I had.

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The 5th

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The 7th

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And boom! The 9th! Contams.. lame, weird. Dont know how they got in there.. so the red circle is the wrong colored nasty I got..

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And the green circle is where I (in a glove box, with a flame sterilized scalpel) cut out some (hopefully) clean myc and moved it here! To it's new home!

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Ok cool. Thanks for reading about my first project, any tips are always welcome, more first timer trials to come!
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#11 jkdeth

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Posted 11 July 2019 - 01:44 AM

The tubs can do do great! I hope you pasteurized that hpoo one well.

#12 MushLearner

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Posted 11 July 2019 - 08:36 AM

I hope I did too! I tried so we shall see! What is your fav pasteurization tek?

#13 jkdeth

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Posted 11 July 2019 - 11:37 AM

My favorite isn't used a lot these days, I fill the pc with quart jars of substrate, close it up and bring it up to a boil. Once I get 10 minutes of steam I turn it off. About an hour later, I do it again. Then I leave it overnight.

#14 MushLearner

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Posted 11 July 2019 - 02:24 PM

Well if I get a contamination this time i know what I'll try next time.. I used a small cooler, filled the cooler with boiling water to heat the walls, dumped it, filled it with my mixed sub, then poured boiling gypsum water over it and wrapped it in a towel. Let it set overnight.. so well see. Hopefully it's good enough.

#15 roc

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Posted 11 July 2019 - 02:32 PM

I hope I did too! I tried so we shall see! What is your fav pasteurization tek?

I'm still old school and toss my horse shit in an old pillow case, put it in a big pot on the stove and bring it up to 160 degrees for an hour.

Hang the pillowcase up over night and the moisture content is just right after the excess drains out. My understanding of "field capacity" moisture level.

Shit works for me!



#16 MushLearner

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Posted 11 July 2019 - 03:28 PM

I hope I did too! I tried so we shall see! What is your fav pasteurization tek?

I'm still old school and toss my horse shit in an old pillow case, put it in a big pot on the stove and bring it up to 160 degrees for an hour.
Hang the pillowcase up over night and the moisture content is just right after the excess drains out. My understanding of "field capacity" moisture level.
Shit works for me!

Actually that one sounds easier for big bulk subs anyway.. guess I'll need to invest in an aluminum pot for boiling my hpoo. Think what I did will be enough?.. or should i be worried? Nothing i can do now, just watch close and hope.

Edited by MushLearner, 11 July 2019 - 03:58 PM.


#17 jkdeth

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Posted 11 July 2019 - 04:20 PM

On the surface, I'd say not enough, but that doesn't mean it can't work. Just keep an eye on it.

The concern is really bacteria.

#18 MushLearner

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Posted 11 July 2019 - 04:46 PM

The concern is really bacteria.


So if theres a bacterial issue the temperature will be hot, correct? Any other way to easily detect bacterial issues?

#19 jkdeth

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Posted 11 July 2019 - 04:54 PM

Just have to wait it out and see. Excessive metabolites. Pins that rot quick, failure to colonize. Its kind of it will either work, or it won't.

#20 MushLearner

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Posted 11 July 2019 - 10:54 PM

Cool. I'll wait and see then!
Heres the GT in straight cococoir that I bag crushed on the 9th at around 48 hours.

20190711_212638.jpg
And the original pic after I made it for comparison
20190709_204055.jpg

Fingers crossed!
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