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Wild spores


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#1 Boebs

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Posted 02 July 2019 - 06:29 PM

I am very new to agar work with wild spores.
This will be my updates on how this project is going, and any advice would be greatly appreciated!!
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#2 Boebs

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Posted 02 July 2019 - 06:35 PM

Knocked 3 pre made dishes up
2 cubes
1 cope
20190702_193026.jpg 20190702_191812.jpg
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#3 Boebs

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Posted 04 July 2019 - 03:26 PM

Somthing is growing.
20190704_125604.jpg 20190704_125556.jpg
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#4 MycoBabe

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Posted 04 July 2019 - 04:05 PM

What kind of media did you use? Idk what my prob is, i always have issues. Thats after a long career as a professional microbiologist too. My colonies form, just not in the way i want them. Tia!
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#5 CatsAndBats

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Posted 04 July 2019 - 04:25 PM

  Super excited for you @Boebs!

 

gallery_147940_1513_380.png

What kind of media did you use? Idk what my prob is, i always have issues. Thats after a long career as a professional microbiologist too. My colonies form, just not in the way i want them. Tia!

 

What kind of problems are you having babe? Are you subscribed to either of my agar threads?


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#6 Boebs

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Posted 04 July 2019 - 07:26 PM

@mycobabe just premade potato agar plate from amazon lol.
Figured they had a money back guarantee, might as well try to clean up these spores. I used to skip this step with wild collected, but dont have the time to find the perfectly rip cap anymore, also copelandia are almost impossible to get a clean wild print..
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#7 MycoBabe

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Posted 04 July 2019 - 11:48 PM

I am not subscribed to thise threads,.... I will def have to find those. And yes. I used PDA as well. They had the apoearance of a slightly mucoidal gram negative. Never would grow into rhizoids. I suspected contamination and was just going to pull the fungi oit, it never grew tho.
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#8 Boebs

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Posted 05 July 2019 - 06:29 PM

Hmmmm..

20190705_192400.jpg 20190705_192333.jpg 20190705_192300.jpg

#9 Boebs

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Posted 06 July 2019 - 05:56 PM

Any thoughts on whats growing?

Attached Thumbnails

  • 20190706_165012.jpg


#10 Cuboid

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Posted 07 July 2019 - 05:27 AM

Any thoughts on whats growing?

I'd think that most of that is mycelium but top right end blob looks like yeast or slime mold maybe? Transfer from the leading edge of myc at bottom of photo would be what I'd do.

Hopefully someone with more experience will be along...

(Disclaimer: My experience with agar work is very minimal!)
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#11 Boebs

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Posted 07 July 2019 - 06:29 AM

This is my first attempt so it doesn't matter, any helpis appreciated!
It looks like healthy myc to me.
I figured today to tomorrow il do a tranfer

#12 Boebs

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Posted 13 July 2019 - 11:20 AM

2nd transfer looks clean20190713_113643.jpg
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#13 bezevo

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Posted 13 July 2019 - 12:34 PM

WOEW! LOOKS GOOD !


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#14 ItBeBasidia

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Posted 13 July 2019 - 10:54 PM

With wild prints I usually do one, add it to the collection and do another and use that for culture usually without cleaning needing to be done. The idea being that most contaminants drop on the first print and the second being much cleaner. I know it's frowned on by some, but I've spore swabbed straight to grain many times with no contamination. It's especially effective with fresh spores because they germinate quick. These are wild Psi. Cyanescens that were swabbed print to substrate with no contamination. Looks like yours are going great so far! 8798016b0eaefc12e8e8b52ae7f4b499.jpg
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#15 Boebs

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Posted 14 July 2019 - 07:26 AM

Thats what i used to do, make multispore prints tossing the first couple.
Thank you fpr the motivation!
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#16 ItBeBasidia

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Posted 14 July 2019 - 11:22 AM

Anytime! I love grows from wild spores and can't wait to see these come to fruit! Is that last pic the cope?
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#17 Boebs

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Posted 14 July 2019 - 04:28 PM

This is the cope.. does not look right at all compared to the cubes20190714_120805.jpg

#18 Boebs

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Posted 14 July 2019 - 05:03 PM

Other was from an all white cube

#19 ItBeBasidia

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Posted 14 July 2019 - 05:27 PM

That cube's gonna be exciting! I've barely started with copes this year and have had nothing but problems on agar. Manure water in the agar mix really helps though. I'd say that center colony is most promising.
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#20 PanPiper

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Posted 29 July 2019 - 08:23 AM

Boebs, I have been working with wild Copes on PDA for the last year and I've had some that look like your plate and its always been a contam.  Cope myc is more delicate than Cubes but I have never used manure water.  Homemade boiled potato with karo works perfectly fine.  Due to the delicate nature of the myc I always make 12 plates at a time so I am sure to get a few good plates.  Usually I will transfer a couple times until I get aggressive fast growth before I put it on milo/rgs grain.  My best luck has been starting that way then doing grain to grain when I find a winner.  It's really obvious with Copes when its right b/c it is very aggressive and wispy myc will devour the grain.  Despite what some people say you can shake it at 1/3 colonization.  In my experience its binary with Copes...if it isn't super aggressive and fast on the grain I dump b/c a lot of time it won't fruit at all.  It's better to cut your losses.   Good luck

PDA Agar Copelandia
Copelandia PDA
Copelandia harvest





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