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agar work lions mane


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#61 piggy

piggy

    Mycophiliac

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Posted 17 September 2019 - 11:39 AM

thanks for making my log a hot topic. or it might just be me clicking on myself... lol



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put this bottle out of the ref. ref was at 4C. will propagate tomorrow. it was a grain to grain bottle (G2). it has wood chips in it. the wood chips might be contaminated with oyster spawn. i feel iffy on this bottle. its not cleanly eaten. it might have oyster mushroom in it. i will spawn another bottle along side it.



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this set looks good on the outside. no contamination so far. there are almond nuts used as grain. along with wheat grain i believe. took a small peek inside each bottle. it looks like the lions mane is having trouble eating the almonds. will keep observing for now.

i might just remove the almonds using a blade on the weekend.... not confident at all. i will use almonds as a substrate next time mixed with wood chips.


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first generation bottle looks good. raised it from agar to grain to spawn.

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looks like oyster mushroom contamination.

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the controversial chocolate agar. looks like some growth in it. but as i said before it takes 30 days for anything substantial to form on my end

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i feel more comfortable with this bottle. it has more uniform growth. same color all around.... same grain to grain (G2) same date


Edited by piggy, 17 September 2019 - 12:54 PM.


#62 piggy

piggy

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Posted 18 September 2019 - 01:06 PM

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harvested this today. will cook tomorrow. its in the ref now.

 

 

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put eggshell in one of the substrate bottles. the eggshell is for calcium. some growers claim is essential for many harvests.

 

all 4 bottles were put in pressure cooker. stopped the heat 1 hour after the pressure cooker started releasing steam. again the emergency valve blew up.

 

the HEPA air filter was open for approx 1 hour at full blast. the indicator when i started was at 001 particulate. i sprayed the air with 70% alchohol before starting. flamed my blade before each transfer. wiped the table with alchohol. rubbed my hands with achohol also rubbed alchohol with tissue on the bottles.

 

the HEPA filter was kept running as i was doing my transfers. it was turned off when i was finished.

 

 

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i prioritized on this better looking bottle. put it into 3 bottles. including the one with the eggshells. pictured it with flash and without flash.

 

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placed the better looking bottle into these 3  bottles. i have pictured them for reference.

 

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this is the iffy bottle. it has different colors of white. making me believe there are two types of mycelium inside.

 

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i placed a sample of the iffy bottle in here. i will throw the grain G2 jar if it has another type of mushroom in it

 

 

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i placed the iffy grain bottle in the ref. it is also now labeled as "iffy" in addition to the date. there is also a spawn jar marked "iffy".

 

thanks for reading.

 

yes i am wearing a mask. it is my SOP now.


Edited by piggy, 18 September 2019 - 03:46 PM.





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