I am pleased to present my first ever grow log here. Would love to get some good feedback and hopefully show some success down the line.
I've been on a five year break from this hobby, and it feels good to be back at it. I was lucky enough to be gifted with 3 PE clones on agar, as well as two syringes: one of PESA and another of Malabar.
I decided to make some BRF jars, which I PCed at 15 psi for 30mins, to test out the spores. On the first run, I made 4 jars and innoced three of them with approx 2ml each of solution. I set the fourth aside as a control. On day 4-5, signs of growth were noted along with what looked like bacterial contam. The control jar showed no signs of contam.
A side note: I innoced these jars in open air, flame sterilizing in-between jars and wiping with 70% iso in-between holes.
A further note: I have a sourdough culture at home, which I thought might be causing some problems with airborne contaminants.
Currently, around day 19 after inoculation, they appear to be consuming whatever bacterial contam is in the jars at the innoc points. Photos attached.
On another trial-run, I decided to prepare a 4% dextrose solution and PC at 5 psi for 20mins. This LC was innoced with a wedge of PE clone taken from agar in a SAB while in a cleaned room. My clean work is not as good as it once was, but the LC looks clean as far as I can tell. Pic attached.
Finally, I innoced 5 more jars with PESA using approx 1.5ml each. These were innoced in a SAB in the same conditions as the agar transfer, just to be safe. However, after 4-5 days, the same yellowing around the innoc points occurred next to myc, which also germinated around the same time. Pic attached.
Now, my main question is whether you all think it was my methods or the syringes? I do not know where they came from so I have my reasons to doubt them. But I also have reasons to doubt my own procedure.
...which is why some ideas/views/thoughts would be appreciated.
Peace n Love