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Official Copelandia/Panaeolus Thread


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#61 Asura

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Posted 09 August 2019 - 10:12 PM

I'm at day 7 with my pan cyan aussie's and nothing either. .

I've got pan fever real bad!

 

ItBe, how are you fruiting these? With cyans, I usually get pins on day 5. This is almost like clockwork. At 7 days I will start scrambling  :biggrin:  Usually, the first thing I do, is drop the humidity way down in hopes that

I can get more evaporation off of the substrate surface. I'll let the casing get a little dryer than I normally would and then start watering everything by hand. I guess if you are doing this in a tub without a humidity

controller of any kind, you are probably at the mercy of your environment. Hang in there man. I'd give it at least 10 days.


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#62 ItBeBasidia

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Posted 10 August 2019 - 02:21 AM

Currently I have one that I spawned to black kow manure and straw in a plastic rotisserie chicken container and another that's spawned to feral horse manure in a small shoe tote.

The one in the shoe tote I moved outside because some how a couple of fungus gnats made their way in. I put more dense polyfil and now it seems fine, but I keep it out just in case. I've been monitoring the casing and misting as it starts to dry (usually once a day)

The temp for the indoor one is usually 74f.
The outdoor can get up to 90f at times.

I just PC'd a bag of manure/millet that I plan to lay a small shoe tote with once it's colonized instead of spawning.

Here's some photos of the spawn, containers, and casing. I hope some pop up soon. 63d3955e01fee1ea9c5034a8a235fedc.jpg6d87fe9ae46471ffa168411aa3f0d592.jpga8b3cd468df7b214434053cee988a584.jpga5c9081b95cba5adef5172d637968d68.jpg

Edited by ItBeBasidia, 10 August 2019 - 02:31 AM.

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#63 Asura

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Posted 10 August 2019 - 08:42 AM

Maybe it's your indoor temps? In my experience, cyans need a high rate of evaporation off of the surface to trigger pinning.

My fruiting temps are usually 77-82F. I don't fruit in tubs but what I look for is the casing layer drying out regularly. Even in my "automated"

setup where I have high humidity and a fogger going, I still have to mist regularly. 

 

I've tried the Black Kow in the past with not much success. Just a few fruits. But take that with a grain of salt. It could have been

an error on my part and not the black kow. As long as you have a decent amount of straw in there I would think you should

get some pinning.


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#64 jakeoncid419

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Posted 10 August 2019 - 10:55 AM

Pan senguio
Day 5 of FC
F3C1D702-676C-40E0-879E-31208A46B5D6.jpeg
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#65 ItBeBasidia

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Posted 10 August 2019 - 11:29 AM

Yeah that was my first and only go with black kow. I didn't like it at all. There was hardly any grass and there was wood and rocks in it. It would also turn into a muddy goop when hydrating it.

#66 jakeoncid419

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Posted 12 August 2019 - 04:10 PM

Pan cyan Alabama

2BE43E46-AF6B-4769-AE86-7D473531AB22.jpeg
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#67 jakeoncid419

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Posted 12 August 2019 - 04:13 PM

Black cow can be really tricky you wan sun dried aged manure. Pans easily go anaerobic so a fulffy sub no more than 2.5” thick is ideal

E321B524-EE1B-48F9-9AF7-5231A5683AF2.jpeg

#68 ItBeBasidia

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Posted 12 August 2019 - 04:27 PM

I can't handle this! These photos are driving up my pan fever!

I dont know if I can Cope anymore

Edited by ItBeBasidia, 12 August 2019 - 04:29 PM.

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#69 KapnDank

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Posted 12 August 2019 - 04:47 PM

Lmao I feel ya. Working on putting together my jartha. Put it off for a while because of financial burdens but now they're lifted I can hardly stand waiting for my pieces in the mail lol
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#70 ItBeBasidia

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Posted 12 August 2019 - 10:04 PM

I've tried shaking a couple of grain jars of my pan aussie's to speed up colonization and noticed they dont really recover that quick.

Is this a pan thing? I'm letting the rest go without shaking until spawn time.

#71 jakeoncid419

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Posted 13 August 2019 - 01:42 AM

Shaking jars is a good way to weed out poor genetics. Don’t over shake but I hit at 30% then the ones that recover will again at 90% when I shake again. Those that do not recover would not have produced well even if they hadn’t stalled. Pan ms are kinda like sids prone babies. More frequently than cubes good looking cultures will stall for no good reason. But it’s worth the hunt for good genetics 71762CC8-81FA-420F-AE90-88D189D36126.jpeg 26B0B66D-F8FD-457B-A165-F54CE690D0D5.jpeg 921B1F69-4360-433D-995C-07FFC35F1D71.jpeg

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  • 50FFBF0E-EB89-44E7-900A-95990264FF04.jpeg

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#72 Rac3k

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Posted 13 August 2019 - 05:30 AM

i have some cakes colonizing from MS in a mycoboxes ,they started good, then almost stoped and going extremely slow, no shaking ofc. I was sure It was too much moisture, but could also be just spores then?

They are almost done after 40 days  :biggrin:


Edited by Rac3k, 13 August 2019 - 05:31 AM.


#73 jakeoncid419

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Posted 13 August 2019 - 01:10 PM

Yup can ups be genetics. I’ve never put pans on cakes so not really my area of expertise, but too much moisture will def ruin your project

#74 ElrikEriksson

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Posted 13 August 2019 - 01:28 PM

I’ve found pans do well with peptone, my recipe is 1000ml distilled water (950g)
32g dextrose (sugar)
16 g malt extract
16 g peptone
8g yeast
20 g agar
10 g potato starch

(Optional below)
.3 g ammonium citrate
.2 g thiamine
.2g magnesium phosphate
This is max Nute I’ll reduce sugars by up to 50% depending on what I’m doing

I too will still be around I’m going to put my pan tek on here for y’all.

 

That's interesting, quite a bit different from the "official" Pan. cyan. culture media from the CRC 'Handbook of Microbiological Media, Fourth Edition' (2010)

 

 

Dung Extract Agar
(DSMZ Medium 781)
Composition per liter:
Agar ............................................................................................15.0g
Malt extract...................................................................................5.0g

Ca(NO3)2·4H2O........................................................................0.72g
MgSO4·7H2O................................................................................0.5g
K2HPO4 .......................................................................................0.25g
Peptone .........................................................................................0.1g
Dung extract...........................................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Dung Extract:
Composition per 150.0mL:
Horse dung.............................................................................. variable
Preparation of Dung Extract: Add an average sized piece of
horse dung to 150.0mL water. Gently heat and bring to boiling. Boil for
2 hr in a water bath. Filter. Use immediately.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Panaeolus cyanescens.

 

This media was apparently developed exclusively for Pan. cyan. and they were kind enough to use a pressure cooker setting on their autoclave, like they knew trippers were watching :laugh:

 

Can peptone be replaced with anything OTC? My home lab has basically every inorganic ingredient to all the culture media I see but no peptone. Will whey protein work? A lifetime supply would cost me less than even the smallest quantity of any grade of microbiological peptone.

Thanks :smile:



#75 ilikethings

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Posted 13 August 2019 - 04:36 PM

Very cool post Asura!  I’ve only worked with Cubies (Mazatapec/Mazatec being my top general purpose choice) but I’ve had a couple Pan-syringes just sitting here bc I am not familiar with preparing the subs needed to pans.  From what I recall, they tend to require poo-based subs but I’m ignorant on them as of now.  I have about 25lbs of alpacha poo but its almost three years old and the person that gave it to me put worms in it so idk if it would even work well after I sift out the worms.  it looks like very black soil at this point. 

 

Pans are definitely my next step.  And I agree with Asura as to why more attention should be given to Pans, i.e. twice the Psilo-concentration by weight!

 

I’ve got one Panaeolus Cambodginiensis and one Panaeolus Cyanescens.  Looking forward to trying them!

 

@k5h re camera data: the simplest move to avoid any lingering worry re meta data, I’d suggest you use a standalone camera with no wifi/Bluetooth connection.  Then you won’t have any worries re location or other doxable data.

 

I did not even know that the other site went down!  sheesh.


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#76 jakeoncid419

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Posted 16 August 2019 - 02:12 PM

Bisp 8882DDBA-BC26-429E-AB30-284191149B9C.jpeg
Pan Alabama 66638071-F023-4315-AD3A-708CD1E98638.jpeg 01214E50-6701-4BC5-BBA9-DE8DEE386E9A.jpeg A04AC1F1-3C7C-4F98-976C-35B5FA5A1495.jpeg
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#77 Asura

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Posted 17 August 2019 - 12:08 AM

Your bisporus trays look like a forest compared to mine...although I am up to five pins now lol.


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#78 Nicked

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Posted 18 August 2019 - 12:52 PM

Sorry Foster...I'm still here!  :tongue:

 

I, personally, just don't have a lot to post. Hope these bisporus will give me the gold. Next up I have Tropicalis followed

by a whole lot cyans. Of particular interest to me are some wild prints I received from the same area as the Transkei

cube. The area is also known at the Wild Coast...so hopefully pan cyan var. Wild Coast will be a thing and I can spread

the spores far and wide. But this is down the road a bit. I do one little grow at a time so hopefully before the end of the

year.

I'd love to see that! I'm from SA so any strains from here really make me smile :) On that note has anyone grown the Pan cyan from KwaZulu Natal in SA? the local spore supplier has them available on their website and I am very tempted to try growing Pans when I get a little more experienced at this


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#79 Asura

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Posted 18 August 2019 - 01:02 PM

The guy I got the original prints from is from KwaZulu Natal. All I know is that my prints are from wild

samples and "the post office really sucks here"  :biggrin: Didn't know there was a local spore vendor there. Wonder what

they've got.


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#80 Nicked

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Posted 18 August 2019 - 01:09 PM

This is a fantastic thread. Love all those pics Asura and Jakeoncid ... hopefully one day I'll have some pics like that to share :) 


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