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What in the Green Hell is this?


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#1 FesterAddams

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Posted 13 August 2019 - 07:34 AM

Green core with fuzzy myc. These are MS from syringes.

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#2 coorsmikey

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Posted 13 August 2019 - 07:54 AM

That there is time for a flow hood or something. Most likely operator error with them being consistent like that. Hard to get 4 dif syringes that came that way unless you got them from a single person on Reddit or FB. There is no bacteria or yeasts growing yet either, that tells me that the syringe were either pure trichoderma on all of them or you had a moldy bag of bread when you poured those. Just guesses really, been up working all night and don't have my filters on! What is the real story? 25 ways not to grow now?


Edited by coorsmikey, 13 August 2019 - 07:58 AM.


#3 sandman

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Posted 13 August 2019 - 08:18 AM

complete and total agar contamination like that is from either incorrect sterilization (too short pc time, not venting the steam before closing weight or valve) or from pouring in a dirty area (too much airflow in a still air box, open air, flowhood not working, etc)


Edited by sandman, 13 August 2019 - 08:21 AM.


#4 FesterAddams

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Posted 13 August 2019 - 11:14 PM

All of the above. I’ve noticed that my tiny 35mm dishes don’t contaminate nearly as fast as my 100mm dishes do.
I guess the myc growth rate has a little to do with this.
Interestingly, these (except for the Z strain) were treated with H2O2 yet all four grew this crap at the same time.

#5 sandman

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Posted 14 August 2019 - 08:25 AM

h2o2 is useless stop doing that. Here we have exhibit 1. 


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#6 FesterAddams

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Posted 14 August 2019 - 04:50 PM

It’s all in the trash now...




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