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Attempt cloning from 2 year old samples


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#1 ElPirana

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Posted 04 September 2019 - 09:37 AM

I am attempting some clones of some 2 year old dried cubensis mushrooms.  I want to say thank you first to Traderkain for inspiring me to give this a try https://mycotopia.ne...anescens/page-1
 
The samples are both two years old - or 1 year and 11 months to be exact.  These were originally dried in open air in my garage in front of a fan, then moved to a dehydrator in my basement.  Once they were cracker dry, they remained in a ziplock bag with fresh desiccant that was occasionally replaced to keep the fruits cracker dry.
 
One sample is Burma and the other is Ecuador.  I tried splitting the stem and only get the sample from the unexposed inside.  It was a little hard to do, surprisingly more difficult with the Ecuador even though it was larger, it fell apart on me and pieces would fall in the SAB.  But in the end I managed to get something from each sample onto agar.  The agar recipe is just standard MEA, nothing special.
 
6BBC9162-4037-4054-AAD3-4052B2BD4A3A.jpeg

I took the samples on 8/31/19.
 
Three days later (pictures are from yesterday, 9/3/19) I can see some results.
 
Here's a picture of the Burma, looking good so far.
 
E57FF299-ECDC-4236-801B-C8EB1FA8B75F.jpeg

And the Ecuador, very small specs scraped to agar and maybe I can see some growth??  I'll know for sure over the next day or two.

0132B5BE-7AA2-435F-8017-8859AF8FA513.jpeg
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#2 deeezy

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Posted 04 September 2019 - 10:16 AM

Nice. And very interesting. Do let update us with the results ElPirana!  :rolleyes:


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#3 ElPirana

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Posted 04 September 2019 - 04:28 PM

Nice. And very interesting. Do let update us with the results ElPirana!  :rolleyes:

Thanks deeezy.

I took another look at the Ecuador, there are two definite spots of mycelium growth now.
7E5A9679-2073-4534-A937-A4565BE5E1D4.jpeg
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#4 ElPirana

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Posted 06 September 2019 - 08:06 PM

Day number 6.

The Burma is moving right along. A good growth of myc and one small spot of something else away from the mycelium.
A39A3B14-96E5-4E4C-849E-665B948EF9CE.jpeg
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The EQ is also doing good, and like the Burma there’s something else growing to the side.
04528AB3-3474-4ABE-904D-856E692E022A.jpeg
CB25647B-7752-4D49-BE6A-A9D47B68CB51.jpeg

I’m not going to wait much longer to do some transfers out of these jars. Hopefully I’ll have a little time tomorrow.
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#5 ElPirana

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Posted 08 September 2019 - 01:14 PM

I did a transfer from each jar. Here’s what they looked like before the transfers:

Burma
A9564618-26BC-4FF0-9D13-B848E99D85F7.jpeg

Ecuador
596A7794-32DB-4272-A075-B32B693545C1.jpeg
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#6 ElPirana

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Posted 17 June 2020 - 08:08 PM

Way overdue for an update on this.

After a couple transfers of each of the agar plates, I inoculated some LC jars from each. Then noc’d up some grain jars from LC, made some mini-tubs and .... contamination in all of them! LOL

Had a coupla months that were busy so I delayed, then starting back in on it I wondered if the LC was contaminated. So I put a tiny squirt of the Ecuador on some agar, and inoculated more grain jars from agar wedges. Back to the mini tub with a coir/verm sub and this time I got some pins. Here’s a clone of one pin:

7DA56A81-D458-43B6-A96C-0E73A427F8A9.jpeg

The tub is still on its first flush, but no contamination yet.

I had some big doubts along the way. The mycelium grew real fast, but very thick. None of that beautiful rhizomorphic growth we like to see. I had also run a couple BRF jars noc’d up from the same agar plate. I decided to sacrifice them to get a better inspection, they smelled great, just like they’re supposed to.

Plans moving forward:

I will grow out this clone, get some more tubs going. I also want to get some spore prints, then there’ll be no stopping this strain revival. I also need to go through the same process with Burma, I think I still have those jars of LC.
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#7 Arathu

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Posted 17 June 2020 - 08:13 PM

Awesome man agar is some beautiful stuff.........

 

A


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#8 ElPirana

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Posted 19 June 2020 - 05:43 PM

Awesome man agar is some beautiful stuff.........
 
A

Yeah man, agar kicks ass. I’ve often thought that if there was only one part of a grow that I could choose to do, it would be agar work. If only there was someone else to do the rest lol.
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#9 makinbones69

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Posted 19 June 2020 - 07:57 PM

Me me me!
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#10 ElPirana

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Posted 27 June 2020 - 11:43 AM

Time to button up this project.  First, just to follow up on the clone, I let it grow out a bit and have made a couple transfers.  Here's the agar before transfers:

 

IMG_6847.jpg

 

 

Then the second flush turned out much better than the first.  I was able to get five good spore prints.  Here's the flush and one of the prints:

 

IMG_4845.JPG

 

IMG_4846.JPG

 

Now that I have some spore prints, which was my ultimate goal out of this grow from a two year old dried fruit, my possibilities are endless!


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#11 Severian

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Posted 27 June 2020 - 12:49 PM

This is so cool. I didn't know this was possible.

 

And wow! It is possible! It's wild to think that a dehydrated mushroom still has life in it!


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#12 ElPirana

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Posted 27 June 2020 - 01:58 PM

This is so cool. I didn't know this was possible.
 
And wow! It is possible! It's wild to think that a dehydrated mushroom still has life in it!

Mushrooms are badass!!
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#13 425nm

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Posted 27 June 2020 - 02:44 PM

Cooool! I've been meaning to do this with a blue meanie cap I was given.


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#14 Arathu

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Posted 27 June 2020 - 05:04 PM

Very nice work!

 

A


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#15 2Ape2

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Posted 27 June 2020 - 05:33 PM

Really nice work!  

 

You mentioned you didn't get the rhizomorphic growth but it grew fast.  Any ideas why that may  have been that way?  


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#16 TVCasualty

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Posted 04 July 2020 - 01:41 PM

This was definitely worthy of getting a "Magic Shroom" award. Congratulations!


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#17 Severian

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Posted 13 November 2022 - 12:32 PM

I didn't see you mention- but I assume you took samples from the dry fruit? No need to rehydrate before putting it on agar?
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#18 ElPirana

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Posted 14 November 2022 - 04:48 PM

I didn't see you mention- but I assume you took samples from the dry fruit? No need to rehydrate before putting it on agar?


That’s correct. The mushrooms had been cracker dry, kept in a container with desiccant during those two years.

The agar has enough moisture to rehydrate and allow new growth.
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#19 Severian

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Posted 14 November 2022 - 07:24 PM

Looks like I've a date tommorow!
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#20 Arathu

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Posted 15 November 2022 - 06:10 PM

The implications, or perhaps my own projections,..... that's probably a damned fine line of research to continue if I've ever seen one.....

 

Very cool stuff indeed....

 

A






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