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Attempt cloning from 2 year old samples


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#1 ElPirana

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Posted 04 September 2019 - 09:37 AM

I am attempting some clones of some 2 year old dried cubensis mushrooms.  I want to say thank you first to Traderkain for inspiring me to give this a try https://mycotopia.ne...anescens/page-1
 
The samples are both two years old - or 1 year and 11 months to be exact.  These were originally dried in open air in my garage in front of a fan, then moved to a dehydrator in my basement.  Once they were cracker dry, they remained in a ziplock bag with fresh desiccant that was occasionally replaced to keep the fruits cracker dry.
 
One sample is Burma and the other is Ecuador.  I tried splitting the stem and only get the sample from the unexposed inside.  It was a little hard to do, surprisingly more difficult with the Ecuador even though it was larger, it fell apart on me and pieces would fall in the SAB.  But in the end I managed to get something from each sample onto agar.  The agar recipe is just standard MEA, nothing special.
 
6BBC9162-4037-4054-AAD3-4052B2BD4A3A.jpeg

I took the samples on 8/31/19.
 
Three days later (pictures are from yesterday, 9/3/19) I can see some results.
 
Here's a picture of the Burma, looking good so far.
 
E57FF299-ECDC-4236-801B-C8EB1FA8B75F.jpeg

And the Ecuador, very small specs scraped to agar and maybe I can see some growth??  I'll know for sure over the next day or two.

0132B5BE-7AA2-435F-8017-8859AF8FA513.jpeg
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#2 deeezy

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Posted 04 September 2019 - 10:16 AM

Nice. And very interesting. Do let update us with the results ElPirana!  :rolleyes:


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#3 ElPirana

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Posted 04 September 2019 - 04:28 PM

Nice. And very interesting. Do let update us with the results ElPirana!  :rolleyes:

Thanks deeezy.

I took another look at the Ecuador, there are two definite spots of mycelium growth now.
7E5A9679-2073-4534-A937-A4565BE5E1D4.jpeg

#4 ElPirana

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Posted 06 September 2019 - 08:06 PM

Day number 6.

The Burma is moving right along. A good growth of myc and one small spot of something else away from the mycelium.
A39A3B14-96E5-4E4C-849E-665B948EF9CE.jpeg
EDCBA34D-3EBF-477A-B1C7-2A84EFCD9349.jpeg

The EQ is also doing good, and like the Burma there’s something else growing to the side.
04528AB3-3474-4ABE-904D-856E692E022A.jpeg
CB25647B-7752-4D49-BE6A-A9D47B68CB51.jpeg

I’m not going to wait much longer to do some transfers out of these jars. Hopefully I’ll have a little time tomorrow.
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#5 ElPirana

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Posted 08 September 2019 - 01:14 PM

I did a transfer from each jar. Here’s what they looked like before the transfers:

Burma
A9564618-26BC-4FF0-9D13-B848E99D85F7.jpeg

Ecuador
596A7794-32DB-4272-A075-B32B693545C1.jpeg
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