So I have decided to start a thread for all of my agar work. I would like to have a single place so I do not have to start multiple threads for every question or hijack anyone else's threads with pestering. It will give me a place to keep up with my experiments and isolation's. Whilst having an amazing community to critique my work at the same time! Criticism, and advice is completely welcome! I have been working with these amazing life giver's for too long to have not gotten into agar. I see a lot of newer members jumping right into agar, and bulk! I commend you all. The wise does at once what the fool does at last.
So.. at last.
This should be quite the journey. I will be starting by using the no pour technique and doing my transfers in a SAB. I have many hours inside of my SAB doing my best to perfect my aseptic technique, and agar should allow me to see where I sit with my skill set is inside of it.
I am starting with the following:
Panaeolus Cambodginiensis - in a spore syringe
Panaeolus Cyanescens (Alabama) - spore print *Gifted from a great Pan expert, and a generous individual from here on topia
Golden teacher - currently growing - The plan is to transfer a clone to some jars, and collect some prints for future plates/jars
The following prints are two years old, and have been kept in a book bag in the bottom of a closet. A true test to cleaning up an old dusty print.
Golden Teacher - Spore prints from a previous grow
Koh Samui - Spore prints from a previous grow
I'm currently cookin' up the following:
Paul Stamet's MEA from TMC
I split the recipes in half for my 500ml Erlenmeyer Flask
- 10 grams of tan malt
- 1 gram of nutritional yeast
- 10 grams of agar
- 0.5 grams of activated charcoal *Not included in the traditional TMC MEA recipe (can my AC users weigh in here on the amount used - pun intended )
I also have some potatoes boiling on the stove so I can start making some PDY while the MEA is in the pressure cooker. Also following the Paul Stamet's recipe from TMC
(PDY) Potato Dextrose Yeast Agar
- the filtered, extracted broth from boiling 300 grams of sliced potatoes in 1L of water for 1 hour * adapted for a 500ml flask
- 5 grams dextrose sugar
- 1 grams yeast (optional)
- 10 grams of Agar
I have some pictures coming, along with the exact brands, and prices of the supplies. I am an aviation technician by trade so lets talk tools! I would love to see how, and why everyone uses what they use!
Lesson number one, and always number one. Experience is the best teacher. It seems I dumped the agar powder in too fast. I should have slowly let it slide in and stirred it along. Instead my measured amount went right down the funnel causing some clumps. So I am betty crocker shakin' and stirrin' the fuck out of this hahaha.
Thank you all for visiting my piece of this great pie.
Edited by cybele, 10 September 2019 - 02:33 AM.