Thirty seven more days have passed in this experiment since I last posted, and much has happened.
Being new to working with agar, and not knowing what all to look for I am keeping it small. That allows me to stay organized, but using these larger jars still has it's problems. With that being said I decided to shelf the Pan Cambo cultures as most appeared weak, and one never germinated. I plan to focus on one exotic species, and one cube species until I have an isolated, and coveted culture.
As with everything in this hobby one step forward, most likely means more questions! Most of my time spent will be gaining familiarity with the cultures on agar, and furthering the process of watching the culture's entire life cycle.
There is a brown discoloration around the 12 o clock position of this jar that I am unsure of. It was one of the original cultures germinated from a spore print. I took multiple transfers from it, before the discoloration. At 55 days I expected more growth as this seemed to work halfway across the jar and just stopped. It may have to do with the thin pouring of MEA.
Once again this is an instance of gaining familiarity. I do not believe that this is contamination, however it is easy to spot the discoloration. Can anyone speak on this culture?
This culture is another from the original spore print. At 55 days this is the type of growth I envision. However, this jar was opened multiple times, and transfers were taken. Does this appear suspect to anyone?
One thing I have noticed is that after about a week on the food colored PDY the cultures seem to make weird mountainous configurations then collapse, and turn green. Nearly 80% of my PDY cultures are not viable, unless I transfer fast.
I also started three new cultures of Golden Teachers so I can familiarize myself with cube myc, and get some decent genetics. I started with an old spore print.
Hope you all enjoy the post, and pictures!
Edited by cybele, 05 November 2019 - 02:53 AM.