In-Vitro Jar Tent Tek
- Colonized Jars
- Micro perforated bread bags (preferably gusseted)
- Square plastic nursery pots
- Plastic cups (that you can cut holes in without cracking)
1. Begin with fully colonized jars.
- I won't tell you how to mix your sub, but I find it this tek works best with sub on the fluffy side and prefer fine verm for jars
- I find larger jars work best for this tek, 1qt+
- I prefer polypropylene jar in general and they may filer light differently and be a factor in preventing side-pinning. I would recommend Bel-Art Wide-Mouth 1 L (1 qt) Polypropylene Mason Jars as they are autoclavable and heavy duty
- Make sure jars are filled to the top as you'll be scooping out of them
- Have an extra empty, clean/sterile jar ready for the colonized sub you're scooping out as you can save it and still get it to produce most of the time
2. Prepare a clean workspace.
3. Remove caps from jars.
4. Take a clean measuring spoon, wipe with alcohol, and scoop out the top 1/2 inch to 3/4 inch of colonized sub from the jar, placing it in the clean jar you had ready or discarding it.
Try to keep the sub as level as possible when scooping
5. Water the jar with an amount of distilled water roughly equal to the amount of sub you scooped out.
If there is any water still pooling on the top of the sub after a day or so pour it off.
6. Cut or poke a ~1/2 inch hole in the bottom of a plastic cup, mist the inside, and fit it snugly over the top of the jar.
7. Place jars in a clean location that get at least 6 hours of indirect sunlight per day and mist the inside of the plastic cups daily or whenever they're dry.
8. Within two days to two weeks you should begin to see primordia (tiny mushroom bumps). As soon as you see this, remove the cups, mist the inside of the bread bags thoroughly, put the jars in the nursery pots, and secure the bags over the pots. Mist 2 times daily or when dry.
- I find more headroom is better for the tents to increase FAE, minimum 8 inches for a 1qt jar
- If you wont be around to mist, you can leave moist paper towels in the pots for moisture
Please feel free to add and refine!