I am in the process of fabricating some plastic lids.
The plan is to have plenty of clean bulk inoculate using the Mycelial Syringe tek. After I isolate a good culture on agar I would like to then transfer a clean wedge to a sterile grain jar. The grain jar will then be used to create mycelial syringes. I plan to follow the tek to a T with the exception of making the SHIP obsolete. I plan on doing that by making some changes to the lids.
I am using the following:
- half-pint jar
- wide-mouth plastic lid, grey
- self-healing injection port, translucent/clear
- syringe filter .22 micron pore size
The syringe filter should stop the jar from coming under vacuum. If I feel resistance when pulling the sterile water back up then I know we need more air flow. If that occurs I can place a ship through the same injection port as they are built for longevity. Either way we are all going to find out!
[This is the exact design, my parts are just different colors. I will post a picture of my lid's as soon as they are done, and cured.]
I am using the following thread's:
BuckarooBanzai's mycelial slurry tek https://mycotopia.ne...-inoculant-tek/
My only question is the grain prep. I would like to use popcorn, but I have only worked with oats. Fashster went into some detail with his grain prep, but I believe his grain hydration revolves around his tyvex absorbent lids. What is the best way to prep, and hydrate popcorn, on an extremely small scale. I will still be using oats as my primary spawn. I am just using the popcorn to "shred" the myc for the syringes.
Edited by cybele, 17 September 2019 - 04:06 PM.