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B+, GT, and Huautla invitro bags


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#21 TabbyBoy

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Posted 08 November 2019 - 05:28 PM

So bit of a pickle.
All of the other bags are clearly contaminated except for this one.
It's got some mushies coming out already as well. They're dying to fruit.
I'm gonna try it. Just this one
I leave for basic in a couple months and I think this should be ready before I leave if I do this now.
Wish me luck!93ef2fde8a421f43ef12c8de1a880130.jpg79c50e1466dd468849b7acd65498dd85.jpg

#22 FunG

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Posted 09 November 2019 - 01:17 PM

That bag is starved for f.a.e .... some cubensis do much better in low fae environments then others.....that's what I was told by a invitro bag grower along time ago.

Not sure which strains are better for invitro fruiting but the reason that mushroom looks like that is because of the lack of f.a.e up that and you should notice a considerable change in their appearance on 2nd flush....

The only other cause for long skinny mushrooms is bacterial contamination but I'm thinking it's just the cubensis variety you have not liking the invitro fruiting.

Maybe try spawning the bag to a tub.
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#23 coorsmikey

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Posted 09 November 2019 - 04:48 PM

Fruiting Cubensis on straight grain can also produce small thin fruits as well. Not sure what's in the bags but wanted to note that  is another cause of poor yields no matter how much FAE is available. Thats why so many cultivators add coir or verm to their subs, It will make more water available to support larger mushrooms and or yields.


Edited by coorsmikey, 09 November 2019 - 04:51 PM.

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#24 TabbyBoy

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Posted 09 November 2019 - 08:48 PM

Fruiting Cubensis on straight grain can also produce small thin fruits as well. Not sure what's in the bags but wanted to note that is another cause of poor yields no matter how much FAE is available. Thats why so many cultivators add coir or verm to their subs, It will make more water available to support larger mushrooms and or yields.

So next time I should add some verm to it to keep the humidity up?

Edited by TabbyBoy, 09 November 2019 - 08:48 PM.


#25 FunG

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Posted 10 November 2019 - 12:54 PM

I didnt realize it was straight grain...

Yea, you can still probably get away with adding it in, just pick the mushrooms and add in a bulk substrate like verm/coir or manure.
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#26 coorsmikey

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Posted 10 November 2019 - 02:52 PM

Yeah Grains are generally used to spawn to substrates that are more supportive of fruiting. Didn't you use "All in one" bags your first couple of go arounds? I was thinking that you may take this spawn bag and add to a larger commercial made substrate bag.


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#27 TabbyBoy

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Posted 11 November 2019 - 05:42 PM

Yeah Grains are generally used to spawn to substrates that are more supportive of fruiting. Didn't you use "All in one" bags your first couple of go arounds? I was thinking that you may take this spawn bag and add to a larger commercial made substrate bag.

That's what I did.
There was actually 2 that didnt look contaminated so I used those, bleached the grow area just in case and spawned them.
Weirdly, outgrow sent me manure and straw based sub for the extra one they sent.
I used that one and one that wasnt straw.
Is straw made for other strains? I really disnt know it had straw until I was mixing it up and it was to late

#28 FunG

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Posted 11 November 2019 - 06:09 PM

Straight straw works great for cubensis, that's not going to be a problem.

Manure/straw works the best of all substrates in my opinion.
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#29 TabbyBoy

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Posted 11 November 2019 - 06:34 PM

Straight straw works great for cubensis, that's not going to be a problem.

Manure/straw works the best of all substrates in my opinion.

That's what it is I might have worded it wrong.
One of them is manure/straw based and the other is manure based

#30 FunG

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Posted 11 November 2019 - 07:01 PM

The vendor separates them for liabilitie reasons.

The grain bag was intended to be spawned into the bulk bags ;)

Your on the right track
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#31 TabbyBoy

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Posted 11 November 2019 - 08:54 PM

The vendor separates them for liabilitie reasons.

The grain bag was intended to be spawned into the bulk bags ;)

Your on the right track

I know this is what I did last time.
The first time I tried the all in one bag and failed.
Second time I did this way it worked.
Third time around (now) 3 bags contam
But 2 haven't and those are now spawned.
I'll be checking on them in maybe 5 days
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#32 TabbyBoy

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Posted 15 November 2019 - 01:14 PM

My shipment came in.
They didnt only send me the grains, they sent me a whole set of substrate too!
So my idea now is to go ahead and spawn the contaminated ones to the old subs and inoculate these new ones and spawn them to the new subs.
If all of them grow, I'm gonna leave about 10 bags worth.
If the contam 3 dont grow ill still have 7.
It's a win win.
I'll probably find another spot for the contaminated ones that way they dont infect my new ones.

#33 TabbyBoy

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Posted 15 November 2019 - 01:17 PM

Now to order more spores for these bags.
Which strains should I get?
Can pans grow on rye?
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#34 TabbyBoy

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Posted 15 November 2019 - 01:28 PM

I might actually wait until next grow to do the pans as I'll have my setup built by then instead of the makeshift one I'm working with.
Still need to choose 2 strains though.
What are some good strains that are more flexible with conditions?
Except for b+ since I've already grown those

#35 Moonless

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Posted 15 November 2019 - 01:36 PM

PES Hawaii is a personal favorite of mine. If there are strains from South/Southeast Asia or Northern Africa that might be something cool to look into. The cubensis species is supposedly native North Africa and migrated with cattle hearder people into India.


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#36 FunG

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Posted 15 November 2019 - 01:51 PM

I wouldn't transfer contaminated spawn to bulk, you'll just be spreading a contaminate.
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#37 PJammer24

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Posted 15 November 2019 - 02:23 PM

Is the bag 100% grain or have you already spawned to the bulk substrate in the bag shown in the photo? I found it difficult to discern from what I was reading.

 

If you have already mixed spawn and substrate, you don't have to move it to a tub... Just poke some holes in the side of the bag to provide additional FAE... You don't even have to fill the holes with polyfil or cover them with microtape... They are contam resistant at that stage... An old fart around these parts takes a broadhead arrow, stabs the bags three or four times, sets them on the shelf, and lets them do their thing...

 

Good luck!!


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#38 TabbyBoy

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Posted 15 November 2019 - 03:10 PM

I dont use bulk, Ive been doing invitro in the bags. 

If i were to spawn the contaminated bags it wouldnt be to bulk i would be putting it in a bag by itself and i plan to put it somewhere different and not with the rest of the bags that arent contaminated. 



#39 Billcoz

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Posted 15 November 2019 - 10:40 PM

I wouldn't transfer contaminated spawn to bulk, you'll just be spreading a contaminate.

YES. You don't want to keep any contaminated spawn/sub around the grow area even in the bags. Don't open them inside either, contam spores will get all over you and your house. 


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#40 TabbyBoy

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Posted 17 November 2019 - 03:02 PM

I wouldn't transfer contaminated spawn to bulk, you'll just be spreading a contaminate.

YES. You don't want to keep any contaminated spawn/sub around the grow area even in the bags. Don't open them inside either, contam spores will get all over you and your house.
Yeah, fuck it. I'll toss them. I think 7 bags will be enough lol. Should get me a good 5 ounces or so in the end maybe more. I got about 2 or 3 out of the 2 bags last time all 5 flushes included




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