To start this thread Ill provide some background. I never use grains only BRF/PF tek.
I started with B+ MS syringes to pf tek, with some success
I then used leftover MS to inoculate liquid cultures.
The liquid cultures appeared clean. (visible mycelium no discoloration, nothing floating on the surface)
Using these LC s I inoculated PF tek jars.
I crumbled 3 jars into a ziploc bag with sterile media.
After the rest of the jars are 100% as well as the bag, I spawned to a monotub.
I used Damnien's bucket tek and added 3TBS Gypsum, 1 TBS Olive oil, and 1 cup of spent coffee grounds (coffee grounds were freshly used and could very well be the reason for the Trich)
(got impatient and kept opening the bucket towards the end to check temp)
(Also an accidental low spawn to sub ratio)
Crumbled and mixed evenly and added a thin layer of sub over the top.
It looked good for 9 days
on the 10th day Trich appears (circled in picture) (Did not notice at first)
on the 11th day Trich takes over (picture taken outside, before DE-CON)
I quarantined this tub.
on the 13th day Trich sporulates (picture taken before outside, before DE-CON)
In the same grow room I have another test ziploc bag, which I crumbled two stalled cakes and squirted a few cc's of LC over top. This was colonizing well(100%) in 5 days there was a bit of cobweb so I dunked it in a 10% peroxide solution for 3 hours then rolled in verm and spawned to a SGFC
I spawned the brick into a SGFC.
2 days after spawning the brick im seeing this
Could be bruising, guess I will know in 24 hours
Now in my mind there are 3 possibilities:
1. The bag was contaminated and got into the tub (not as likely).
2. the Trich from the tub got into the SGFC/bag.
3. My liquid cultures were bad making both end products bad.
My main questions are:
What is the likelihood that the LC was bad, but the cakes were flawless?
Can BRF cakes look wonderful for 2 full weeks and still harbor trich?
Or was it more likely, lazy pasteurization/coffee additives?
This has all caused me a lot of stress Lots of time and effort has gone into this and im looking for answers. Just want a little advice and peace of mind moving forward.
Edited by macgyver, 09 October 2019 - 07:59 PM.