Myceliated Grain TEK
Posted 09 May 2022 - 06:34 PM
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Posted 10 May 2022 - 01:36 AM
If I do end up getting a freeze dryer though, I will definitely try it on a batch of myceliated grain and report the results.
I’d love to hear of the results. It may be difficult to objectively measure a difference in potency though. At the end of the day it’s a water extraction from mycelium grown on cheap kernels of grain. If you want to get fancy, filter it and simmer it down into a gob of honey or chocolate and it’ll be well-preserved, concentrated and pleasant-tasting.
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Posted 27 May 2022 - 07:01 PM
I have a question, as this is my first time growing semp, and also first time growing for the myceliated grain itself. When growing out the semp grain, is it typical to have it start showing areas of discoloration? I'm about 2 and a half months in and I have some spots that are starting to look dusky. When I look close with a bright light, they don't look like contam, at least not like a mold. Still fairly smooth mostly white mycelium, no fuzz or visible spore transfer. Just kind of gray and the mycelium not nearly as bright white as they were, jars were uniformly white and well consolidated. Also getting some yellow/orange discoloration from metabolites, I did expect that. I also have 4 half gallon jars that I inoculated G2G from one of the jars from this generation, and they all look great, dense white mycelium, no signs of contam, though they are about 6 weeks behind these:
Edited by dantisocial, 27 May 2022 - 07:14 PM.
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Posted 28 May 2022 - 03:52 AM
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Posted 24 September 2022 - 03:59 PM
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Posted 14 October 2022 - 01:13 PM
Already the pleurotus LC is showing lots of growth, whereas both sv and the Thai no where near as much
I'm of the belief that pleurotus is just in general a far more vigorous culture, but I feel I should be seeing a bit more growth in the other lc jars.
I've been swirling 3-5 times a day since inoculation, at room temp here so around 65-82.
Posted 14 October 2022 - 02:28 PM
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Posted 11 November 2022 - 08:38 PM
I think I may have tinkered too much before really establishing a successful baseline. The first jar I processed (pictured above), I didn't follow the tested process. My very inexperienced bioassay found it to be active but weak. Where I varied was briefly rinsing the myceliated grain in lemon juice before dehydration. The thought was that it would prevent a lot of the oxidation inherent in the drying process. I believe that prevented oxidation as intended, but it also had the unintended consequence of either degrading the active alkaloids or binding/changing polarity such that they don't extract nearly as easily in water. I have a couple other jars from the same batch that I will keep it simple with and see if it was just me overthinking things and screwing it up.
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Posted 14 December 2022 - 04:03 PM
Figured I'd post this here- as it's concerning a Semperviva Culture
I checked on an SV liquid culture the other day- (sitting in my fridge since I last inoculated my grain jars, 2 months ago) and the culture have.... Consolidated? If this is contamination I've never seen LC jars contam this way before.
First photo is the culture at rest- Second photo is a motion shot of the blobs (Which appear quite solid) swirling around.
Edited by Severian, 14 December 2022 - 04:08 PM.
Posted 15 December 2022 - 01:44 AM
Severian, some people grow aged semperviva/subtropicalis mycelium in LC. It forms a solid layer at the top of the LC that you can harvest. Other species will do it too. So most likely that is Subtropicalis myc clumping in there.
Edited by YoshiTrainer, 15 December 2022 - 01:45 AM.
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Posted 17 December 2022 - 01:59 PM
Posted 17 December 2022 - 05:34 PM
Posted 17 December 2022 - 06:07 PM
Posted 17 December 2022 - 09:24 PM
I dehydrated my first round of sv a week ago- I was seeing some contamination in a few of the jars and pulled the plug 2 days under 2 months.
I've only had the chance to test it twice, and only with 1tbsp in tea both times, but it seems definitely less potent than 1g of cubensis ... Perhaps a longer run next time.
Sounds like I have to get me a natalensis culture!
Edited by Severian, 17 December 2022 - 09:27 PM.
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Posted 17 December 2022 - 10:52 PM
Posted 18 December 2022 - 11:25 AM
And for clarity, when you say 'lemon juice/water' extraction , you are referring to TWO SEPARate extraction methods , correct? Or is there a hybrid version of which I am unaware?
Edited by Severian, 18 December 2022 - 11:28 AM.
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Posted 18 December 2022 - 02:00 PM
Water with lemon juice/citric or ascorbic acid (acetic in a pinch). You could follow the first part of the TEK then make ice cubes, gummies, honey or just consume it.
Posted 18 December 2022 - 02:29 PM
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