521 replies to this topic

[DonShadow]

I’m pretty sure the niacin is included to increase absorption. I could be wrong but that’s what I’ve always thought. Paul talks about it in the most recent Joe Rogan interview.

[Asura]

Don, I have some lion's mane on plates right now. Really wispy stuff. Not sure if it's just the culture or

how it is supposed to look. It's very light.

 

Anyway, I am guessing there is no need to let it consolidate for months and months on the rice?

How long are you leaving it before you dehydrate it?

[DonShadow]

Lion’s mane mycelium looks very weak at first, particularly on agar. Just give it time. It’s a weird one, sometimes it starts to fruit in vitro before it even fully consolidates. The mycelium is just very light and difficult to see, but once it consolidates it gets very thick and meaty like other mycelium. I leave mine to consolidate for two months, but longer might be better. YMMV.

[DonShadow]

I always use cacao before or during velada. I also prefer cacao over coffee in the morning, but I will abstain for a couple of days before doing a velada to bring tolerance down a little. Thanks Samwise for sending me this article :)

 

https://www.forbes.c...d/#14365eb46087

[Samwise]

I’m pretty sure the niacin is included to increase absorption. I could be wrong but that’s what I’ve always thought. Paul talks about it in the most recent Joe Rogan interview.

 

Liking the sound of the mycelium mix smoothie Don! As far as I'm aware, Paul's inclusion of niacin is based more on theory than hard evidence. Not knocking it, and there may indeed be an effect or synergy there, but I consider the lion's mane and psilocybin microdose the more important ingredients here (and cocao a good shout for another brain nourishing substance). Going to 'noc up some lion's mane LC in next few days, very much looking forward to trialing this myself. :)

[DonShadow]

I really like the niacin. I'm not too concerned if it's helping or not because it feels really energizing and pleasant in the body. The flush pretty much disappears by the third day. The stack is amazing. I'm not shitting you, it sort of feels like cheating. My favorite way to consume so far is 1/4 teaspoon semperviva rice and 3 teaspoons Lion's Mane rice brewed in hot cacao with cinnamon, ground chili, a dash of salt, a few drops of vanilla extract and honey... I highly recommend it.

On Friday I had 8 tablespoons of Ps. caerulescens mycelium on brown rice, brewed in tea. After about 12 minutes brewing it started to get mushy though. I squeezed as much liquid out of the rice as I possibly could, so I didn't lose much. Strangely it wasn't very potent though, maybe the equivalent of 1.5 - 2g cubensis, nowhere near the potency of 8 tbsp semperviva grain. I have it on whole oats as well, so I'll see if that works any better. I'll also try just eating the rice next time... I really dislike having that quantity of empty carbs in my gut during a velada, but it's worth a try.

Edited by DonShadow, 09 December 2019 - 05:37 AM.

[Samwise]

Nice one Don, I'm very much looking forward to sampling this stack myself! Sounds like something that is going to be pretty easy and worthwhile to incpororate into my life, it's nice being able to grow one's own supply. I'll be intrigued to hear how you get on with this over time...your recipe sounds great! 

 

Thanks for sharing your Caerulescens experience...a friend and I sampled some too, also on rice...it was only 6 weeks old following inoculation. We started off with 4 tablespoons of the grain gently simmered, and ingested in the form of cocoa...this first dose produced subtle effects so two hours in we had a top up, of another 3 tablespoons...so 7 tablespoons in total, but staggered. In my case, it boosted the experience a little, but it was still quite subtle...but my friend had a relatively short lasting, but immersive and powerful experience that she described as feeling quite reminiscent to her of ayahuasca, based on her prior experiences with that. Clearly our sensitivities vary! I appreciate incubation time wasn't long so this may well have impacted potency, but it seems like your experience was not a great deeper than mine. I concur that the Hoogshagenii/Semperviva seems to be a great deal more potent by weight. It will be interesting to compare eating vs tea consumption...the tea certainly seems smoother on one's system, but my feeling is that ingesting it may yield a longer lasting experience. It will be interesting to see how the oats compare.

 

I'm in the process of inoculating grain jars (rice, oats and rye grain) with Azurescens, Cerulescens, Mexicana, Hoogshagenii/Semperviva and Zapotecorum. My hunch is that the Mexicana won't be as potent as the Semperviva, and I'm quite intrigued about the Azurescens and Zapotecorum. I'm also making up a load of lion's mane LC. 

Edited by Samwise, 09 December 2019 - 11:38 AM.

[bezevo]

 perhaps  oats is a good idea it is higher in L-Tryptaphan  hummm  many have suggested that  Quinoa much higher in L-Tryptaphan  might boost potency . , but it has  slimy soapins  .  but even higher  in Amino Acids  than oats ..  but even better its cousin kaniwa is even higher in L-tryptophan . and it doesn't have the slimy soapins of Quinoa .  maybe add 20% kaniwa to  oats or brown rice ?

i  ate both of these grains  In Peru in the Sacred Valley near Cuzco at a Home hosted dinner at a farm i visited . 

ok just throwing that out .

 

https://www.healwith...ition-facts.php

 

Edited by bezevo, 11 December 2019 - 11:18 AM.

[DonShadow]

In my experience, the method works by far the best when making tea using Ps. semperviva on whole oats or whole barley (husk intact). Brown rice works well too but not for making tea, it gets too mushy.

[ethnobotanist420]

So you do this with lions mane mycelium as well? That perfectly answers my question of how to grow mycelium for consumption after hearing Stamets talk about lions mane mycelium having more hericenone and erinacine coumpounds than the fruits.

Awesome thread!

I am still weary of consuming myceliated grains, tho.... probably for no good reason. I mean you can tell when spawn is being overtaken by contaminants but what if it’s only slightly contaminated? Slight enough that the mycelium can overtake it still.... is there any chance of contamination in such low quantities, or dead contamination, causing harm?

I assume the risk is pretty negligible but it still runs through my head lol

Can you shed any light on this?

Edited by ethnobotanist420, 12 December 2019 - 01:15 AM.

[DonShadow]

I’ve eaten heaps of myceliated grain, and have suffered no ill. Make sure your master culture is clean and put your trust in the ever burning fire of Jah.

[Direct Link]

Edited by DonShadow, 12 December 2019 - 02:39 AM.

[Moonless]

I really apprechiate the mail tek don shadow. Hows your experience been with the mail? Do you include your adress for return?

[ethnobotanist420]

I’ve eaten heaps of myceliated grain, and have suffered no ill. Make sure your master culture is clean and put your trust in the ever burning fire of Jah.

https://youtu.be/wDSnazUSBv4

Thanks man! I think I can do that :)

[DonShadow]

I really apprechiate the mail tek don shadow. Hows your experience been with the mail? Do you include your adress for return?

It works well in my experience, provided that sterile technique is followed and only fresh unopened baggies are used. I never put my real address on any letters containing spores or agar. I use a fake address and fake name so that it does’t look suspicious. Nothing says “I am full of something illegal” like a letter without a return address.

Regarding any concerns over the purity of a culture, just make sure that you are thorough with your transfers when starting from spores or an agar wedge. Always isolate clean mycelium before inoculating liquid culture, and consider testing your LC by placing a drop on agar before inoculating grain. Once it has been well established that your LC is clean, store a syringe of it in the fridge, and always use that syringe to inoculate new LC’s. If you are starting with an LC syringe that was mailed to you, test a drop on agar before inoculating LC or grain.

And yes, this tek works very well for growing Lion’s Mane on brown rice. I’m also experimenting using whole oat groats (whole oats without husk) for Lion’s Mane, as oats are far more nutritious than rice, which has very little soluble fibre and is mostly starch.

Edited by DonShadow, 12 December 2019 - 05:38 PM.

[ItBeBasidia]

So the theory behind mycelium having more of the goods is because the mycelium has to develop immunity to stave off other organisms and pathogens in its substrate/environment.

If the mycelium has only been cultured on steril media than it must be immunologically naïve.

Do you guys think leaving the lid off of a jar of lions mane grain for a week before dehydrating will enhance hericenones and erinacines?

[ItBeBasidia]

Also,

Gonna sacrifice a U.K. Psi. Cyanescens on millet jar for the cause.

It's already bluing from over consolidation.

[DonShadow]

Thanks ItBeBasidia, I’m curious to hear how that goes for you. You mean Psilocybe Cyanescens right?

[ItBeBasidia]

Yes, psilocybe cyanescens.

It came from the print of this beauty

[bezevo]

here's a small chart from DNA sequencing Allen Rockefeller did showing how closely related these are .  perhaps all possible candidates for  the Myceliated Grain TEK . testing .

 

[Auxin]

Do you guys think leaving the lid off of a jar of lions mane grain for a week before dehydrating will enhance hericenones and erinacines?

That seems a bit random and uncontrolled. What about shaking and injecting some brine from live culture pickled cabbage one to two days before drying?

Another option is you can get little packets of Bacillus subtilis var. natto endospores for making natto. Bacillus subtilis is a soil bacteria that fungi would 'recognize' and possibly use as a trigger to mount defenses and its harmless to humans.

Of course, the question is what function do hericenones and erinacines serve. If they are to inhibit bacteria the above should work, if they are to inhibit competitor fungi then yeast, another gourmet culture, or Aspergillus oryzae would work. If they are some kind of signaling device (they seem a bit non-volatile for that) or are a mechanism in a stress response cascade then applying a stressor to prompt their production would help. Dilute coffee or tea has been noted to trigger cAMP production in mushrooms, showing it to trigger stressor response systems. Dilute aspirin might work too.

Wheres a gas chromatograph when you need one