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Macgyver's Myco Madhouse


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#1 macgyver

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Posted 07 November 2019 - 09:02 PM

Hello Mycotopians!

I've seen a couple threads where people will post their projects and update on their progress, and it has inspired me to do the same. I enjoy conversing with the people on this forum and everyone has been super helpful in getting me to the place I am today. I've learned a little bit, and will continue to do so. Through my successes and my failures alike, I appreciate all input and advice!

 

This is my biggest project at the moment: “operation wildfire”. I used BRF jars to colonize substantially bigger BRF bags, which were then spawned to this tub. Which has been colonizing for 10 days now. Not sure when I should introduce FC... How is this one looking?

 

(All B+ for now)

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Edited by macgyver, 07 November 2019 - 09:09 PM.

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#2 macgyver

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Posted 07 November 2019 - 09:05 PM

I also fruited one of these “wildfire bags” in a SGFC and I'm about to finish my second flush (I think)

wondering how I will know when the flush is truly done. I harvested a bunch of big guys today, but I still see a few pins on the surface. Also not sure if I should go for a third!

 

What do you guys think?

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#3 onediadem

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Posted 07 November 2019 - 09:57 PM

Dunk it. Even with the pins on it.


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#4 macgyver

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Posted 07 November 2019 - 11:41 PM

Dunk it. Even with the pins on it.

Decent size pins should I wait a day or two to see if they grow? Some look like they want to but most came and went at the same time



#5 Moonless

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Posted 08 November 2019 - 08:27 AM

Nice positive affirmations



#6 macgyver

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Posted 08 November 2019 - 03:42 PM

Pins that are left have not grown since they appeared so I'm going to give the bricks a short dunk. They don't seem dry and still have some weight to them. Second flush was about half the weight of the first, but the mushrooms were much larger over all.

 

I also used a syringe to suck up some of the pooling water from the sides and corners of the monotub. It was slightly gelatinous but crystal clear, I think that is a good sign? no metabolites?


Edited by macgyver, 08 November 2019 - 03:42 PM.


#7 macgyver

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Posted 08 November 2019 - 05:51 PM

I'm starting to get into agar, first I did a couple test "plates" in those disposable 2oz food containers. I inoculated this one with some LC about 5 days ago, not sure if it is cube my that's growing in here or not

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#8 RutgerHauer

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Posted 09 November 2019 - 05:49 PM

Looks like Cubensis myc to me, but that picture isn't the clearest! Here is my LC on agar, Colombian strain.

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#9 macgyver

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Posted 09 November 2019 - 05:56 PM

I transferred a few strands from the biggest blob to a new plate, with a better agar recipe so hopefully I'll be able to get a better look! Agar is super cool, I've been reading other posts like catsandbats and he has some crazy stuff going on. Hoping to get some more rounds of plates going.

 

Do you reuse those plastic dishes at all?


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#10 RutgerHauer

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Posted 09 November 2019 - 06:05 PM

I use glass. I'm not sure if you can reuse plastic petri dishes, they are made to use once.

#11 macgyver

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Posted 09 November 2019 - 06:11 PM

I use glass. I'm not sure if you can reuse plastic petri dishes, they are made to use once.

Yeah I'm hesitant to do it, but I have a few syringes that have been used again and again.

 

If the plastic dishes can withstand boiling water, it may be possible. Might be worth the risk cause I see a bunch of transfers in my future :biggrin:



#12 RutgerHauer

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Posted 09 November 2019 - 06:31 PM

You need the plastic with that 3 symbol on it and you'll probably be fine.

Edited by RutgerHauer, 09 November 2019 - 06:32 PM.

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#13 FunG

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Posted 10 November 2019 - 11:03 PM

The ghetto alternative as I mentioned in that pm is 250ml wide mouth canning jars, that's if your either wary of ordering glassware petri dishes or cant for some reason.

They're abit deep but not impossible to work a culture out in.

#14 RutgerHauer

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Posted 11 November 2019 - 11:35 AM

Also don't drop them when you use glass. Jars might be safer when it comes to that. I lost two already in the last two weeks. Haven't been sleeping much, must be playing its part.

Agar is cool, I am a fan, though I seem to contaminate a significant portion of the dishes I pour/work with. Have to refine the technique I guess..

Edited by RutgerHauer, 11 November 2019 - 11:36 AM.


#15 macgyver

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Posted 11 November 2019 - 02:12 PM

So a few updates. After about 11 days I'm putting both my mini tub and my full sized tub into fruiting conditions. I see a tiny pin in the big tub!!!

Is this light overkill?

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#16 macgyver

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Posted 11 November 2019 - 02:14 PM

And I'm seeing some nice growth on this plate (my first transfer) but there is a hardened ring around the edge. It looks like water hardened from condensation, Is it bacterial? and should I transfer again now?

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#17 CatsAndBats

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Posted 11 November 2019 - 02:18 PM

photo-147940.jpg?_r=1573497045

I'm Agar Rick!

 

Rutger, getting contaminations is very much a part of the agar game. The third jar in the pic below has some bacteria or yeast or whatever. Point is, it happens to all of us.

 

McGuyver, virtually anything that one can think of can be used as nutrition. I come up with new nutrition all the time. One just needs to avoid too much acidity and too much fat IME.

 

post-147940-0-64299400-1573499280.gif

 

The first and third jars, the nutrition is 9g BRF and 2g dried potato with bone, coir, and straw as supplements (catattives) in trace amounts to train them and get them used to their future food.

 

My craziest agar recipe lately was silkworm pupae as the nutrition source.

 

 

 

 

 

 

 

 

 

 

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#18 macgyver

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Posted 11 November 2019 - 02:29 PM

Cat- My mix is still basic 6g LME 6g Agar Agar 300ML final solution. I do a lot of BRF so maybe using BRF as a nute will be my next batch.

 

These are some pictures of the plats I did on the 7th. I'm wondering if the spots I see are bacterial or just hardened spore water, I did a few plates using a scrape from a print, and a few from a spore syringe. Tried to get just a drop but it is tough with a 30mL syringe :biggrin:

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#19 CatsAndBats

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Posted 11 November 2019 - 02:39 PM

I see a ton of bacteria in the pic on the right and I can't tell what's going on in the first pic. There might be viable myc in both actually, but I can't show you where.

 

I do five plates (jars) minimum if I'm germinating because I don't trust anyone's printing including my own :tongue: !

 

 

Oh and no matter what's going on, grab from the bottom of the "1" and get it to a new plate on this one, that's cube myc.

 

post-147940-0-56312800-1573501361.png

 

Especially if you may have contaminants on other parts of the plate.

 

 

 

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Edited by CatsAndBats, 11 November 2019 - 02:44 PM.

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#20 macgyver

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Posted 11 November 2019 - 02:43 PM

I see a ton of bacteria in the pic on the right and I can't tell what's going on in the first pic. There might be viable myc in both actually, but I can't show you where.

 

I do five plates (jars) minimum if I'm germinating because I don't trust anyone's printing including my own :tongue: !

That right plate in the second plate in the center is pretty much straight bacteria right? There's a bunch of the more innocuous looking white bubbles in most of the plates, could that just be trapped moisture once the agar hardened?

 

edit: I tried to get a good sample size for the first batch (need more plates!) These are from prints I took a couple weeks ago so they are fresh but might not be the cleanest


Edited by macgyver, 11 November 2019 - 02:44 PM.





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