Paradox
©
Fisana

Jump to content


Photo
* * * * * 1 votes

B+ mono tubs


  • Please log in to reply
49 replies to this topic

#21 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 02 December 2019 - 08:55 PM

Then you need to increase your shaking of the grain jars.
 
I do around 25% then when it's 75%, which is quit quickly, then at 90% and get the sub ready.
 
 
Peace

Would you give these a shake? I have 4 like this, usually when I shake around 30-40 % I can get it to fully colonize after that and ready to spawn within a week. One of my last gwm tubs I just spawned 6 jars that kinda stalled at 98% cuz I was afraid to shake again, the tub still turned out stellar tho.

Attached Thumbnails

  • DDB2F2E2-66DD-467A-A479-07B8E70AA580.jpeg
  • E2EE3A30-0F9A-454D-A7EF-0E6F9F54777A.jpeg
  • 09B0EFAF-BCE1-4C14-B86D-1E7AC21E1817.jpeg


#22 crazy1

crazy1

    Celestial Traveler

  • VIP
  • 2,344 posts

Posted 02 December 2019 - 09:07 PM

If you can shake those I would then spawn in a couple days.

 

If you can't break it up, then prep your sub and spoon that stuff out and go

 

 

 

Peace



#23 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 03 December 2019 - 07:33 AM

thanks! How hard do you shake it when it’s at 25%? Do you distribute the myc all over or just kind of a light shake

#24 crazy1

crazy1

    Celestial Traveler

  • VIP
  • 2,344 posts

Posted 03 December 2019 - 09:06 AM

You're welcome.

I personally shake it up real good each time. More inoculation points

 

 

Peace



#25 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 03 December 2019 - 08:11 PM

I gave these corumba jars a shake about 4 days ago, they were around 30%, would you consider them 75% now and give them another shake or just let them go

Attached Thumbnails

  • 4B3C0C49-4F99-49EB-BD39-E0C88672E7E6.jpeg
  • 15B36FC1-9439-4ECF-92DF-EBC70F648230.jpeg


#26 PJammer24

PJammer24

    Archetype Novice

  • OG VIP
  • 2,369 posts

Awards Bar:

Posted 04 December 2019 - 08:32 AM

I gave these corumba jars a shake about 4 days ago, they were around 30%, would you consider them 75% now and give them another shake or just let them go

 

You can shake them if you want but I would probably just let them go... They will finish up in a day or two



#27 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 04 December 2019 - 09:15 AM

Sweet, thanks! I’ll be spawning those Corumba early next week, I’ll make another log for those.

#28 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 06 December 2019 - 11:40 PM

There’s a bunch of mutant pins starting in here, does anyone know what causes this? It’s happened before when I last grew these but there was only a couple, and the tubs grew fine. This seems to be quite a bit more tho, I’m hoping it still pulls through. Still looks and smells awesome. I’m using an old print, it’s from 2011 I’m pretty sure, and it’s just about the last of it.

Attached Thumbnails

  • 7CDCF605-5578-4606-8ED4-62FD6718BACF.jpeg


#29 FunG

FunG

    Been there, read that.

  • Free Member
  • 1,055 posts

Posted 07 December 2019 - 12:00 AM

P.cubensis mycelium is so dense and thick it's almost impossible to separate it using a car tire, or the palm of your hand. Once it consolidates pfffft, good luck breaking it apart without your hands or sterilized fork "emphisis" on "sterilized fork".

#30 FunG

FunG

    Been there, read that.

  • Free Member
  • 1,055 posts

Posted 07 December 2019 - 12:10 AM

Aresols like lysol, febreeze, glad etc are well known to cause mutations. I used a bit of my glad last time around my bin fully expecting mutations to occur and yea, they happened.

Everytime I use one, but if you use a liquid based cleaner of various brands (say to clean the surface areas then nothing will happen)

Best to avoid aresol use period but they'll probably figure this out in 30yrs time by seeing how disfiguring it is on myceliums reproduction and mens and women's health and say "magical fungi, lead the way". Cigarette and meth smoke doesnt even cause deformations lol that should speak for itself.

#31 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 07 December 2019 - 12:28 AM

Haha yea Lysol can fuck some shit up pretty bad, the only thing I use is a bit of 99% alcohol to clean with, but it’s only this strain that’s done it, the print came from someone else tho so maybe something happened back then. I’m still hoping to see some actual pins soon tho.

#32 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 10 December 2019 - 10:06 PM

Aresols like lysol, febreeze, glad etc are well known to cause mutations. I used a bit of my glad last time around my bin fully expecting mutations to occur and yea, they happened.
Everytime I use one, but if you use a liquid based cleaner of various brands (say to clean the surface areas then nothing will happen)
Best to avoid aresol use period but they'll probably figure this out in 30yrs time by seeing how disfiguring it is on myceliums reproduction and mens and women's health and say "magical fungi, lead the way". Cigarette and meth smoke doesnt even cause deformations lol that should speak for itself.


Did you still let the tub fruit? I removed some of the mutants and getting some nice normal pins coming through. I’m assuming the mutants aren’t safe to consume?

#33 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 12 December 2019 - 10:09 PM

So I removed a bunch of mutants and still getting a decent pinset, everything still smells good so I’m just letting it go, the second flush might be back to normal. I was doing some reading and I guess petroleum products can cause mutants, I’m thinking it’s possibly that, from not washing my new tub good enough before using, that or possibly stress on the mycelium from
not fruiting them right away and have them over grow a bit when they stalled.

I did the other jars into grow bags to see if they grow a bit more normal tho.

Attached Thumbnails

  • C3A1224E-4315-4E5A-8E3C-27B68C5BA78F.jpeg


#34 macgyver

macgyver

    Mycotopiate

  • VIP
  • 450 posts

Awards Bar:

Posted 12 December 2019 - 10:20 PM

I've had very similar mutants in my tubs, I also have the same lines up the stems (I'm also working with B+). Which makes me wonder if it could be genetic.

 

If we could combine your evenly spread out pin set, and my clusters, that would be a tub to be reckoned with...



#35 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 12 December 2019 - 11:47 PM

Haha yea no kidding! I haven’t seen the stripes before so I’m thinking it’s just the mutation. This tub would be deadly if it was normal and didn’t have to removed all these fucked up pins lol

Attached Thumbnails

  • E01A5E1B-D6A7-42DB-8900-BF3C4BD8CC55.jpeg

  • RutgerHauer likes this

#36 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 16 December 2019 - 07:40 PM

This tub didn’t turn out too bad after all. I picked a bunch of the mutants a few days ago and let the more normal looking ones finish. The sub got tore to shit too, a little saturated so I didn’t dunk it, I’m hoping it hangs on for another flush but it’s pretty rough. it was lacking fae too, it’s hard to pack these holes any looser tho, they could probably be left right open.
Pretty happy with the yield overall tho, the dehydrator is packed full :)

Mush love ❤️

Attached Thumbnails

  • AAD93465-D961-46DD-A7AD-7768057F86CD.jpeg
  • 16AE7CBB-8055-4310-96FA-A42F5E9EB350.jpeg
  • 3D5AD073-88F2-4444-9A22-12E30FD872FE.jpeg
  • E23D5429-48AC-4C9D-B820-A40B47B58E31.jpeg

  • macgyver likes this

#37 RutgerHauer

RutgerHauer

    Knows Nothing

  • Free Member
  • 1,009 posts

Awards Bar:

Posted 17 December 2019 - 03:05 AM

The vertical dark lines, splitting/twisting/hollow stems, weird caps, brown spots, blobs and other deformities indicate a contam like Lecanicillium (old name: Verticillium). It is a harmless one for us so the fruits are alright to eat, it is considered a cosmetic contaminant.

 

Some chemical influences like FunG mentioned are also possible, so I have heard as a comment on my first grows as well - but seeing that it exhibits all the things "dry bubble" is known for I would not draw that conclusion here.

 


When infecting A. bisporus, dry bubble is unable to infect the vegetative mycelium and can only infect the fruit bodies. This means infection must take place in the casing layer, a layer of material, usually mulch put on top of the mushroom. In general, dry bubble symptoms are dependent on the time point of infection, affecting both the type and severity of the disease symptoms. Symptoms are mild when inoculation occurs during casing layer applications, and severe when inoculation takes place 14 days after the casing layer has gone down after the mushrooms have poked through.[3]

 

Interactions between the pathogen and the host in the casing layer can either result in stipe blow-out or dry bubble. Early infections can cause stipe blow-out in which part of the fruit body of the mushroom becomes deformed, accompanied by splitting or peeling of the stipe tissue.[3]L. fungicola also causes totally deformed and undifferentiated white masses of mushroom. If inoculation occurs in the late stage of the mushroom life cycle, symptoms include small necrotic lesions on the cap of the mushroom. Other symptoms may include brown, light brown, or gray discolorations on the cap or the stipe of the mushroom. Infection by L. fungicola does not decrease the weight of the mushroom crop, but has the potential to decrease the total number of mushrooms produced.[3] Therefore, dry bubble is thought of mainly as a cosmetic disease.

 

 

Lecanicillium is a common airborne contam that I see sometimes getting into my agar dishes and have had in a few grows before when I used a casing, fanning a lot and had it pretty much open to contamination from the get go. Insects can carry it around too.

It probably got onto your sub during the later stage of colonization or at the stage of pinning - possibly at the time you started fanning or increasing FAE in some other way.

 

It is probably a good thing you picked the weird ones off of the sub, so that it was not able to spread out as much as it would have otherwise. That is what generally adviced when dealing with this contaminant, as soon as you recognize it, to reduce the effects.

 

I would not advice cloning or printing anything like this, you will most likely take the contam with it if it is in there and will be difficult to isolate a clean culture from it.

 

You can draw your own conclusions about doing another flush with it or not.. If you do, would be best to get it away from your other stuff.


Edited by RutgerHauer, 17 December 2019 - 08:07 AM.


#38 Stencill86

Stencill86

    Mycotopiate

  • Black VIP
  • 220 posts

Awards Bar:

Posted 17 December 2019 - 10:24 AM

Thanks for the info! I assume I’ve been carrying this a little bit on the print, it slowly started years ago but I haven’t used this print in a long time, it’s just about done now so I’ll toss it.

I have some bags and a tray going from this same batch of jars to see if there’s a difference. I was gonna see how it recovers to decide about a second flush, if it doesn’t do anything in a few days I’m gonna toss it. I’ll have another tub ready in a week or so to go in there.
  • RutgerHauer likes this

#39 macgyver

macgyver

    Mycotopiate

  • VIP
  • 450 posts

Awards Bar:

Posted 17 December 2019 - 03:04 PM

I did think it was vert until I saw your pictures, I haven't really seen the stripes on any other pictures. Wonder if there would be any reason that B+ is more susceptible to this? or maybe it's just a crazy coincidence.



#40 RutgerHauer

RutgerHauer

    Knows Nothing

  • Free Member
  • 1,009 posts

Awards Bar:

Posted 17 December 2019 - 03:32 PM

No reason to assume that I think macgyver! Just an external contaminant that got into both of your tubs. It's a common one, it's all around us. Just bad luck I guess.


Edited by RutgerHauer, 17 December 2019 - 04:07 PM.





Like Mycotopia? Become a member today!