50 replies to this topic

[spacecake]

Hi guys...!

I'm going to clone one of my Pans today !
Using 3% peroxide and 2 or 3 parts of one stem.
They will be put in sterilized corn,that after full colonization..will be used the same as a normal colonized jar of corn.

Any comments or idea's ?

I failed the first time using a blender,so this will only be my second try !

[alex27]

why not try a liquid culture..?

[spacecake]

why not try a liquid culture..?

I think that will not be suitable for Pans,I don't say it can't be done but if blending them kills them,then I think sucking live culture in a syringe will do the same thing...

And I think that I cannot use a liquid for spawning poo,..so I have to innoculate corn again.. this will take more time that I do not have !

But if you don't agree just say so !

[golly]

LC worked well for "blue Helix" ..i think its just that pan myc is more sensitive to the presence of bacteria and does not recover from mixing n shaking...If your stems are meaty enough to get a clean interior section then both ways should work..I prefer the popcorn idea though as you can more easily see whats happening ...Then go grain to grain for expansion if ness....gluk..

[spacecake]

Okay Golly ..I have put two pieces of a stem in peroxide for a short wile.
Then I cleaned it off, with distilled water very well...
I couldn't take the inside of the stem,because it was to small.

There I now in the glas along with the corn as you can see in the picture below.
If everything goes well,then I use this jar for spawning horse poo,and maybe take a couple of colonized corn pieces out, for further experiments !

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[golly]

Looks a bit wet Space...If your using just 3% H2O2 then there is a rather high probability of bacterial contam when taking an entire stem cross section...
If you have some more Pans to clone i would attempt to extract inner tissue with a pair of tweezers -just a tiny piece is all thats needed...

[spacecake]

I have the Viets on there way,..I'll try it with them..there are a little thicker right ?

I don't care if this one failes,just a quick experiment to fill my time...

[Hippie3]

good luck!

[golly]

Yeah,the stems of Vietnamese are easily thick enough to split and extract.
The clone will take about 4 days to show new mycelial growth - they usually turn black first and look like they're dead B4 sprouting...
I've lost my fair share o clones which hopefully means - i've learned alot lol....Glukkkkk

[reverend trips]

Man, it would be hard to rip them small stems open and get some inner tissue. Some kind of precision biopsy tool would be nice.
Hope it works out for ya spacecake!

[Hippie3]

chop a cross section then use a sterile needle to separate the strands

[BuckarooBanzai]

Never tried something as small as one of those, but with the bigger guys, letting them dry a little makes the tissue inside the stem shrink away from the exterior and become better differentiated and easier to remove.

I have to say, I've never seen one of these guys in person. Just how small are the stems? From the popcorn jars, they look very small, about 1/3 the diameter of a pencil.

Also, a small spoonful of dry verm will soak up any extra moisture on your popcorn.

Cool thread. Good luck!!!

[golly]

Viets and Goliath have stems of 3mm+ on the best of em, which are splitable ...Seven says he peeled the "skin" off the cap on the smaller ones to get a chunk of meat but said it was a PITA...

[alpiner]

at least that way you can clean it up if need be
is there a specific reasoning that you want to go directly to grain?

[Lazlo]

You don't have to use stem sections. You can always cut a sliver from inside a split cap.

[spacecake]

at least that way you can clean it up if need be
is there a specific reasoning that you want to go directly to grain?

I like speed and simplicity !

And thanks everyone,you all have some simple and good idea's !

BuckarooBanzai..that verm. thing is a good idea too,but I don't think I'll do that this time,..looks wet and it is a little bit,but don't forget it is a close-up picture !

[eu_por_exemplo]

Great Space!

Excelent job :greenboun

[spacecake]

Just a few minutes ago I went to have a look at my corn/clone jar...

I didn't expect to see anything yet,but was very suprized to see signs of growth on all two mushroom stems ! (picture)
Will keep you all updated on the speed of growth !

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[dial8]

Very very cool!

So why did you decide on stem tissue wrather than some from the cap?

[spacecake]

Well,..I do the same things with cubies,so why not ?

Caps may hold some spore that would get in the 'way' and germinate not ?
Do you think cloning a cap is better Dail8..,or just less difficult ?