
Cloning Pan Florida question
#1
Posted 17 September 2006 - 03:42 AM
I'm going to clone one of my Pans today !
Using 3% peroxide and 2 or 3 parts of one stem.
They will be put in sterilized corn,that after full colonization..will be used the same as a normal colonized jar of corn.
Any comments or idea's ?
I failed the first time using a blender,so this will only be my second try !
#2
Posted 17 September 2006 - 03:51 AM
#3
Posted 17 September 2006 - 04:00 AM
why not try a liquid culture..?
I think that will not be suitable for Pans,I don't say it can't be done but if blending them kills them,then I think sucking live culture in a syringe will do the same thing...
And I think that I cannot use a liquid for spawning poo,..so I have to innoculate corn again.. this will take more time that I do not have !
But if you don't agree just say so !
#4
Posted 17 September 2006 - 08:48 AM
#5
Posted 17 September 2006 - 09:26 AM
Then I cleaned it off, with distilled water very well...
I couldn't take the inside of the stem,because it was to small.
There I now in the glas along with the corn as you can see in the picture below.
If everything goes well,then I use this jar for spawning horse poo,and maybe take a couple of colonized corn pieces out, for further experiments !
#6
Posted 17 September 2006 - 10:24 AM
If you have some more Pans to clone i would attempt to extract inner tissue with a pair of tweezers -just a tiny piece is all thats needed...
#7
Posted 17 September 2006 - 11:08 AM
I don't care if this one failes,just a quick experiment to fill my time...
#8
Posted 17 September 2006 - 11:26 AM
#9
Posted 17 September 2006 - 11:30 AM
The clone will take about 4 days to show new mycelial growth - they usually turn black first and look like they're dead B4 sprouting...
I've lost my fair share o clones which hopefully means - i've learned alot lol....Glukkkkk
#10
Posted 17 September 2006 - 11:33 AM
Hope it works out for ya spacecake!
#11
Posted 17 September 2006 - 11:34 AM
#12
Posted 17 September 2006 - 12:15 PM
I have to say, I've never seen one of these guys in person. Just how small are the stems? From the popcorn jars, they look very small, about 1/3 the diameter of a pencil.
Also, a small spoonful of dry verm will soak up any extra moisture on your popcorn.
Cool thread. Good luck!!!
#13
Posted 17 September 2006 - 12:53 PM
#14
Posted 17 September 2006 - 01:03 PM
is there a specific reasoning that you want to go directly to grain?
#15
Posted 17 September 2006 - 01:42 PM
#16
Posted 17 September 2006 - 03:39 PM
at least that way you can clean it up if need be
is there a specific reasoning that you want to go directly to grain?
I like speed and simplicity !
And thanks everyone,you all have some simple and good idea's !
BuckarooBanzai..that verm. thing is a good idea too,but I don't think I'll do that this time,..looks wet and it is a little bit,but don't forget it is a close-up picture !
#17
Posted 17 September 2006 - 06:34 PM
Excelent job :greenboun
#19
Posted 19 September 2006 - 01:31 PM
So why did you decide on stem tissue wrather than some from the cap?
#20
Posted 19 September 2006 - 01:40 PM
Caps may hold some spore that would get in the 'way' and germinate not ?
Do you think cloning a cap is better Dail8..,or just less difficult ?